FastQCFastQC Report
Fri 10 Feb 2017
ERR1631213.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631213.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266973
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT140695.269821292789908No Hit
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG129734.8592928872957195No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT121494.550647443748993TruSeq Adapter, Index 3 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC72082.6998984916077653No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47171.7668453364197878No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT44571.6694572110288308No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC44261.6578455499245242RNA PCR Primer, Index 3 (95% over 22bp)
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC41231.544350926872755No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA22990.8611357702838863No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG17910.6708543560584779No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17180.6435107670064013No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA15510.5809576249283636No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13170.49330831207650205No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT12820.48019837212002714No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA11700.4382465642593071No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT10940.4097792660681042No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT10350.38767965299861784No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT10180.3813119678769014No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG9460.35434294853786713No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9350.35022268169440357No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC8920.33411618403359145No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC8550.32025710465103213No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7740.2899169578946186No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG7430.27830529679031213No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG6820.2554565442947414No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG6690.25058713802519356No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT6450.24159746491218215No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6240.23373150093829714No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6140.22998580380787567No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6110.22886209466874927No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6070.2273638158165807No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5900.22099613069486426No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5700.2135047364340214No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5590.20938446959055784No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5550.2078861907383893No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG5420.20301678446884142No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG5290.19814737819929357No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA5150.19290340221670357No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5100.19103055365149285No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4930.18466286852977642No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA4600.17230206799938572No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4420.16555981316462715No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC4400.16481067373854283No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4250.15919212804291072No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4160.15582100062553142No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4130.154697291486405No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4030.15095159435598357No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000.14982788521685714No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT3870.1449584789473093No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3830.1434602000951407No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3830.1434602000951407No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3810.14271106066905642No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3770.14121278181688784No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3740.1400890726777614No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3700.13859079382559286No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3640.13634337554734No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3520.13184853899083426No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3500.13109939956474997No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3460.12960112071258142No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3390.12697913272128641No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3280.12285886587782284No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3280.12285886587782284No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG3270.12248429616478071No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3250.12173515673869642No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3240.12136058702565428No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3180.11911316874740141No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3140.11761488989523285No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3130.11724032018219072No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3090.11574204133002214No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3040.11386919276481142No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3010.112745483625685No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3010.112745483625685No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA2920.10937435620830571No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2910.10899978649526357No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2880.10787607735613715No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2880.10787607735613715No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2870.107501507643095No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2790.10450494993875785No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT2770.10375581051267357No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2770.10375581051267357No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG2760.10338124079963143No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2760.10338124079963143No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA2700.10113382252137856No Hit
TCGGTAAGGTGATATGAACCGTTATAACCGGCGATTTCCGAAT2690.10075925280833642No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA2680.10038468309529427No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2680.10038468309529427No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTATC200.001839405737.02
AGGATTT200.001839405737.05
ATTACGG750.037.02
TCGCCGA251.2310881E-437.01
TCAGAGT251.2310881E-437.03
CAGACAC403.8144208E-837.018
GATTACG750.037.01
GCTCCGA200.001839405737.03
AGCAACG200.001839405737.02
TTTACAC251.2310881E-437.02
GTAAATT200.001839405737.019
TTACGGG750.037.03
GTTGTAT200.001839405737.01
CTTATAC15650.035.817891
CGTCTTC5800.035.0862137
TTATACA16900.033.059172
GATCAAC401.5939822E-632.3753
GATTAGA401.5939822E-632.3752
TATACAC18100.030.8674033
TAGGACC303.5921443E-430.8333324
Produced by FastQC (version 0.11.5)