FastQCFastQC Report
Fri 10 Feb 2017
ERR1631212.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631212.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences890445
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT42630.47874938935026873No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG37560.42181156612704884No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT36430.4091212820556014RNA PCR Primer, Index 41 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27700.31108041484875537No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26170.2938979948228133No Hit
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC22470.25234573724373766No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC13640.15318183604826802No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC12250.13757166360639905RNA PCR Primer, Index 41 (95% over 23bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9330.10477907113858802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9220.10354373375110197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC6600.026.6287881
GTATCAA11900.026.4285721
CCGTCTT6700.024.57462737
GCCGTCT6750.024.11851936
TATACAC8700.022.7528743
CGTCTTC2150.021.5116337
TGCCGTC7850.020.73885335
GATTACG450.00382514120.5555551
TTATACA9150.020.218582
CGAACTA851.2446635E-619.58823629
GGTATCA3800.019.4736841
ATGCCGT8400.019.16071334
CGGTTCG801.6160036E-518.522
TATATCG609.2345866E-418.55
ATGACGG500.00703366718.520
TTACGGG609.2345866E-418.53
TACGTTA500.00703366718.525
TGCGTTA500.00703366718.537
TATGCCG8950.017.56983433
ATACCGC752.0666071E-417.2666666