ERR1631211.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	ERR1631211.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	534314
Sequences flagged as poor quality	0
Sequence length	43
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT	16523	3.0923763929075414	No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG	14817	2.773088483550871	No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT	13081	2.448185898179722	RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC	8428	1.5773496483341258	No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC	4624	0.8654087296982672	No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC	4474	0.8373353496258754	RNA PCR Primer, Index 33 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3777	0.7068877102228277	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3640	0.6812473564233765	No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG	2106	0.3941502562163821	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1740	0.3256512088397459	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1358	0.2541576675887212	No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA	1332	0.24929161504283998	No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT	1221	0.22851731378927	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1094	0.2047485186613115	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1072	0.20063108958402737	No Hit
ACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGT	1015	0.18996320515651843	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	975	0.18247697047054728	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	787	0.14729166744648278	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	759	0.14205130316630296	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	759	0.14205130316630296	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	740	0.13849534169046665	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	732	0.13699809475327243	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	696	0.13026048353589836	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	685	0.1282017689972563	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	640	0.11977975497553872	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	628	0.11753388456974738	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	615	0.11510085829680675	No Hit
ACTATACTCAATTGATCCAATAACTTGACCAACGGAACAAGTT	557	0.10424581800214856	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCAACG	35	5.623788E-7	37.0	2
GCTCTAG	30	8.303656E-6	37.0	1
CGTCTTC	540	0.0	35.287037	37
GATTACG	60	3.45608E-11	33.916668	1
CTTATAC	1845	0.0	33.590786	1
CGCCGAG	50	7.3159754E-9	33.300003	2
TTATACA	1870	0.0	32.943848	2
ACAGACA	45	1.0781332E-7	32.88889	17
CCTAGTG	30	3.596923E-4	30.833334	17
TAGTGTC	30	3.596923E-4	30.833334	19
AGTGTCC	30	3.596923E-4	30.833334	20
TATACAC	2040	0.0	30.561274	3
ACTTGGT	25	0.005493744	29.6	16
TCCCTTT	25	0.005493744	29.6	37
CAGACAC	50	2.7161695E-7	29.6	18
GGTATTG	40	5.9342732E-5	27.75	33
CCGTCTT	2010	0.0	27.0597	37
CGGATCG	55	6.251212E-7	26.90909	37
GCCGTCT	2055	0.0	26.467152	36
GTAGCTT	35	8.8631565E-4	26.428572	35

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content