FastQCFastQC Report
Fri 10 Feb 2017
ERR1631210.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631210.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences831711
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT45340.5451412810459402No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG42910.5159244016250837No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT37360.44919449183670773No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT37040.44534700154260315No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT33980.4085553756052283TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC25690.30888133017358194No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA15790.189849599199722No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC13230.1590696768468855No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC12270.1475272059645718Illumina Paired End PCR Primer 2 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11940.1435594815987765No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA10500.12624577527530595No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT8960.10772972823492775No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8460.10171802465038939No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACAC9400.026.9627673
ATTACGG856.91216E-1126.1176452
CTTATAC9450.025.6455021
GGTATCA6500.025.0461541
CGTCTTC2500.024.42000237
TTATACA10250.024.1853662
CTAGGGG400.001930740923.1253
CCGTCTT7100.022.92957737
GCCGTCT7200.022.6111136
GTATCAA16800.021.3630941
GATTACG1059.822543E-1021.1428571
TTACGGG1151.3460522E-1020.9130443
ACGGTAC1551.8189894E-1219.0967733
CCGGGCA609.2338887E-418.59
GACCGTT701.2186828E-418.57
AGGACCG609.2338887E-418.55
TGCGGGT609.2338887E-418.521
TTAACGG1002.872548E-718.49999835
TGCCGTC8700.018.49999835
AACACGA500.007033288518.49999816
Produced by FastQC (version 0.11.5)