FastQCFastQC Report
Fri 10 Feb 2017
ERR1631209.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631209.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences440093
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT124112.8200857546018683No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG119562.716698515995483No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT108072.4556173354268305TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC67701.5383112205829224No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC37460.8511837270758681RNA PCR Primer, Index 10 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35300.8021031918253642No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT34730.7891513839120368No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC34690.7882424851111016No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG16470.37423908128509203No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13340.30311775011190817No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12060.27403298848197993No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10690.24290320454994738No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT9650.21927183572563072No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8570.19473156810037878No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT8390.1906415234961701No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8180.18586980479126003No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT7200.16360178416834623No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT6490.14746883045174541No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC6280.14269711174683533No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5810.13201755083584607No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5700.1295180791332741No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA5650.12838195563210503No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC5580.12679138273046833No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5480.12451913572813018No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC5000.11361235011690711No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4740.10770450791082795No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG4720.1072500585103603No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA4530.10293278920591785No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC4450.10111499160404733No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGTTTA200.001840701937.027
CTTATAC15350.033.5048871
TTATACA15700.032.757962
CGTCTTC4550.032.5274737
GATTACG352.3849843E-531.7142851
TATACAC16700.030.6856293
CGCCGTT250.00549270429.625
GGTATCA5100.027.5686261
CCGTCTT14550.025.55670237
CCGCCGT809.749783E-1025.437524
GCCGTCT14750.025.21016936
ATATAGC601.3342742E-624.6666686
TCCGCCG551.899202E-523.54545423
CGGTGTA551.899202E-523.54545434
TCGGTGT551.899202E-523.54545433
ACGGTCA400.001929303923.12511
GTATCAA14050.022.252671
TGATACA502.6984318E-422.22
GGTGTAC603.719562E-521.58333435
GCCGTAG603.719562E-521.58333426
Produced by FastQC (version 0.11.5)