##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631209.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	440093
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.352511855448736	31.0	31.0	34.0	28.0	34.0
2	31.369914995239643	31.0	31.0	34.0	28.0	34.0
3	30.818099810721826	31.0	31.0	34.0	26.0	34.0
4	34.971060662178225	37.0	35.0	37.0	32.0	37.0
5	35.18465642489201	37.0	35.0	37.0	32.0	37.0
6	35.3968888394044	37.0	35.0	37.0	33.0	37.0
7	35.38906776522235	37.0	35.0	37.0	33.0	37.0
8	35.404103223636824	37.0	35.0	37.0	33.0	37.0
9	37.187214975016644	39.0	37.0	39.0	34.0	39.0
10	37.01188839631623	39.0	37.0	39.0	33.0	39.0
11	37.05823314617592	39.0	37.0	39.0	33.0	39.0
12	36.98384205156637	39.0	37.0	39.0	33.0	39.0
13	37.07775174792601	39.0	37.0	39.0	33.0	39.0
14	38.09198510314865	40.0	37.0	41.0	33.0	41.0
15	38.08224625249663	40.0	37.0	41.0	33.0	41.0
16	38.07882197626411	40.0	37.0	41.0	33.0	41.0
17	38.08676802403129	40.0	37.0	41.0	33.0	41.0
18	38.14855269227186	40.0	37.0	41.0	33.0	41.0
19	38.14513523278035	40.0	37.0	41.0	33.0	41.0
20	38.14918892143252	40.0	37.0	41.0	33.0	41.0
21	38.05601543310164	40.0	37.0	41.0	33.0	41.0
22	37.98668008807229	40.0	37.0	41.0	33.0	41.0
23	37.99029068855901	40.0	37.0	41.0	33.0	41.0
24	37.94460489033	40.0	37.0	41.0	33.0	41.0
25	37.90211614363328	40.0	37.0	41.0	33.0	41.0
26	37.72773254743883	40.0	37.0	41.0	32.0	41.0
27	37.60239085829586	39.0	37.0	41.0	32.0	41.0
28	37.56242657801874	39.0	37.0	41.0	32.0	41.0
29	37.51361416791451	39.0	37.0	41.0	32.0	41.0
30	37.378265502973235	39.0	36.0	41.0	32.0	41.0
31	37.269708902436534	39.0	36.0	41.0	31.0	41.0
32	37.12331257256989	39.0	36.0	41.0	31.0	41.0
33	37.04518363164149	39.0	36.0	41.0	31.0	41.0
34	36.955175383384876	39.0	36.0	41.0	31.0	41.0
35	36.958549670183345	39.0	35.0	41.0	31.0	41.0
36	36.94428450350267	39.0	35.0	41.0	31.0	41.0
37	36.815093627937735	39.0	35.0	40.0	31.0	41.0
38	36.73852799294694	39.0	35.0	40.0	30.0	41.0
39	36.62885799137909	39.0	35.0	40.0	30.0	41.0
40	36.39699563501351	39.0	35.0	40.0	30.0	41.0
41	36.343047946683996	39.0	35.0	40.0	30.0	41.0
42	36.20407277552699	39.0	35.0	40.0	29.0	41.0
43	35.37704757857998	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	12.0
10	13.0
11	7.0
12	17.0
13	8.0
14	17.0
15	9.0
16	7.0
17	8.0
18	13.0
19	45.0
20	76.0
21	153.0
22	303.0
23	515.0
24	868.0
25	1436.0
26	2219.0
27	3405.0
28	4938.0
29	6652.0
30	8886.0
31	11270.0
32	14375.0
33	18518.0
34	24211.0
35	31863.0
36	43803.0
37	65011.0
38	103113.0
39	98319.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.99102916883477	23.23804286821195	16.21998077679036	24.550947186162926
2	13.444658288134553	21.951269390787857	41.21333445430852	23.39073786676907
3	13.720054624817937	30.25019711742745	34.51452306671545	21.515225191039168
4	10.081051050573402	19.694019218665147	36.15849377290709	34.06643595785436
5	12.070857750520911	38.60229542392176	34.26866594106246	15.058180884494869
6	26.344204520408187	40.05494293251653	16.85848218444738	16.7423703626279
7	23.432319986911857	33.47746953484831	20.98647331359508	22.103737164644745
8	25.466662728105195	32.574478576119134	22.88448123464813	19.074377461127533
9	22.676797858634426	13.61007786990477	18.113671428538968	45.59945284292184
10	15.173383807513414	27.478282999275155	31.763286396284425	25.585046796927013
11	30.466515032050047	22.48774690803989	24.061277957159056	22.984460102751008
12	20.095525263978296	28.251983103571295	25.558234282299424	26.09425735015099
13	30.583762977370693	19.526100165192357	25.36441161299998	24.525725244436973
14	22.21553171715978	23.06785156773682	25.31192270724597	29.40469400785743
15	25.285110192618376	27.838434149145748	22.064200066804062	24.81225559143181
16	25.637990152081493	25.597089706039405	22.131685802773504	26.6332343391056
17	19.99509194647495	31.225899980231453	23.012181516179535	25.766826557114065
18	23.962889661957814	22.918792164383436	24.99857984562354	28.11973832803521
19	24.825661848745607	25.70070416934602	25.0124405523378	24.461193429570567
20	24.268052434371825	24.52095352573206	25.23398463506577	25.97700940483034
21	25.50301868014261	26.344658969808656	24.90519049382744	23.247131856221298
22	23.929714855723677	30.409027182890892	25.024029012049727	20.63722894933571
23	25.458255414196547	23.822919246613782	25.824996080373918	24.89382925881575
24	23.659090237745204	26.90613120408641	24.89132978711318	24.54344877105521
25	23.621598162206624	26.971571917753746	27.948183679358678	21.45864624068095
26	25.999731874853726	26.216731463577013	24.99517147512003	22.788365186449226
27	24.390072098397383	28.550556359678524	26.59278834246398	20.466583199460114
28	21.284364895601612	26.131294976289105	28.802548552237823	23.781791575871463
29	24.080364831978695	23.761795802250887	25.66343931850768	26.49440004726274
30	26.31239306237545	23.401644652380295	30.047739909519123	20.238222375725133
31	24.078092584976357	25.74819413169489	24.807938322127367	25.36577496120138
32	24.2887298820931	24.48982374180003	30.431295203513802	20.790151172593067
33	25.02130231564692	23.536843349019414	30.39425757737569	21.047596757957976
34	20.24117629682817	27.65165544555355	27.36671567146035	24.740452586157925
35	20.604735817202275	23.851776783543478	35.95353709329619	19.58995030595806
36	26.370108136234844	24.555491680167602	24.45278611566192	24.621614067935642
37	21.593163263219363	25.663666543207913	28.896846802834858	23.846323390737865
38	21.434560422456162	23.25008577732434	29.264496367813166	26.050857432406332
39	24.901100449223232	24.185115418786484	27.060871224945636	23.852912907044647
40	19.884206292760847	23.53025383271263	35.28822317101158	21.29731670351494
41	22.670662791728112	24.541630973453337	26.023363243678038	26.76434299114051
42	21.971265164408432	23.84836841303997	31.880079892204606	22.300286530346995
43	23.311890895787933	22.447073686698037	30.671017262260474	23.57001815525355
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	13.0
1	15.0
2	17.0
3	87.5
4	158.0
5	158.0
6	217.5
7	277.0
8	303.0
9	329.0
10	470.5
11	612.0
12	612.0
13	957.0
14	1302.0
15	3293.5
16	5285.0
17	5429.5
18	5574.0
19	5574.0
20	4550.0
21	3526.0
22	3218.0
23	2910.0
24	3434.5
25	3959.0
26	3959.0
27	4854.0
28	5749.0
29	7054.5
30	8360.0
31	10220.0
32	12080.0
33	12080.0
34	14944.0
35	17808.0
36	19520.0
37	21232.0
38	23535.0
39	25838.0
40	25838.0
41	27452.5
42	29067.0
43	30707.0
44	32347.0
45	33283.0
46	34219.0
47	34219.0
48	34463.0
49	34707.0
50	32997.0
51	31287.0
52	31107.5
53	30928.0
54	30928.0
55	41754.0
56	52580.0
57	35948.5
58	19317.0
59	25762.5
60	32208.0
61	32208.0
62	25238.0
63	18268.0
64	11556.5
65	4845.0
66	3746.5
67	2648.0
68	2648.0
69	2010.5
70	1373.0
71	1037.5
72	702.0
73	478.0
74	254.0
75	254.0
76	186.0
77	118.0
78	93.0
79	68.0
80	53.5
81	39.0
82	39.0
83	37.0
84	35.0
85	31.0
86	27.0
87	21.5
88	16.0
89	16.0
90	13.0
91	10.0
92	5.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	440093.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.44071518261173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.97498330391734	24.5158924142737
2	9.97095183011645	6.269877131804648
3	3.3902433613535865	3.1977502777217497
4	1.6556393441300912	2.0821794025568114
5	1.0582709806723232	1.6636398244670856
6	0.7627646930089768	1.4389120478548507
7	0.5841509393791695	1.2856286316072891
8	0.4928903305045776	1.2397459598126228
9	0.3613148700127748	1.0223998127382627
>10	3.0782296137403975	19.680516136766045
>50	0.4364425461543671	9.462318543666145
>100	0.2167760990832949	12.30260441240358
>500	0.008671043963331796	1.8851937401042418
>1k	0.005780695975554531	4.424230283778019
>5k	7.225869969443164E-4	1.5380527380701032
>10k+	0.002167760990832949	7.9910586423748615
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT	12411	2.8200857546018683	No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG	11956	2.716698515995483	No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT	10807	2.4556173354268305	TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC	6770	1.5383112205829224	No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC	3746	0.8511837270758681	RNA PCR Primer, Index 10 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3530	0.8021031918253642	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3473	0.7891513839120368	No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC	3469	0.7882424851111016	No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG	1647	0.37423908128509203	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1334	0.30311775011190817	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1206	0.27403298848197993	No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA	1069	0.24290320454994738	No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT	965	0.21927183572563072	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	857	0.19473156810037878	No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT	839	0.1906415234961701	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	818	0.18586980479126003	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	720	0.16360178416834623	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	649	0.14746883045174541	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	628	0.14269711174683533	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	581	0.13201755083584607	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	570	0.1295180791332741	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	565	0.12838195563210503	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	558	0.12679138273046833	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	548	0.12451913572813018	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	500	0.11361235011690711	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	474	0.10770450791082795	No Hit
TTATTACCCTCACTGTCAACCCAACACAGGCATGCTCATAAGG	472	0.1072500585103603	No Hit
CCATAGGGTCTTCTCGTCTTGCTGTGTCATGCCCGCCTCTTCA	453	0.10293278920591785	No Hit
GATAGAAACCGACCTGGATTACTCCGGTCTGAACTCAGATCAC	445	0.10111499160404733	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.2722470023381423E-4	0.0
6	0.0	0.0	0.0	2.2722470023381423E-4	0.0
7	0.0	0.0	0.0	2.2722470023381423E-4	0.0
8	0.0	0.0	0.0	2.2722470023381423E-4	0.0
9	0.0	0.0	0.0	4.5444940046762846E-4	0.0
10	0.0	0.0	0.0	6.816741007014427E-4	0.0
11	0.0	0.0	0.0	6.816741007014427E-4	0.0
12	0.0	0.0	0.0	6.816741007014427E-4	0.0
13	0.0	0.0	0.0	9.088988009352569E-4	0.0
14	0.0	0.0	0.0	9.088988009352569E-4	0.0
15	0.0	0.0	0.0	9.088988009352569E-4	0.0
16	0.0	0.0	0.0	0.001136123501169071	0.0
17	0.0	0.0	0.0	0.0013633482014028854	0.0
18	0.0	0.0	0.0	0.0015905729016366995	0.0
19	0.0	0.0	0.0	0.0018177976018705138	0.0
20	0.0	0.0	0.0	0.0020450223021043277	0.0
21	0.0	0.0	0.0	0.0029539211030395847	0.0
22	0.0	0.0	0.0	0.0034083705035072133	0.0
23	0.0	0.0	0.0	0.004544494004676284	0.0
24	0.0	0.0	0.0	0.0054533928056115415	0.0
25	0.0	0.0	0.0	0.005907842206079169	0.0
26	0.0	0.0	0.0	0.006589516306780612	0.0
27	0.0	0.0	0.0	0.008407313908651126	0.0
28	0.0	0.0	0.0	0.017950751318471323	0.0
29	0.0	0.0	0.0	0.035901502636942646	0.0
30	0.0	0.0	0.0	0.05635172565798593	0.0
31	0.0	0.0	0.0	0.10134221630428114	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGTTTA	20	0.0018407019	37.0	27
CTTATAC	1535	0.0	33.504887	1
TTATACA	1570	0.0	32.75796	2
CGTCTTC	455	0.0	32.52747	37
GATTACG	35	2.3849843E-5	31.714285	1
TATACAC	1670	0.0	30.685629	3
CGCCGTT	25	0.005492704	29.6	25
GGTATCA	510	0.0	27.568626	1
CCGTCTT	1455	0.0	25.556702	37
CCGCCGT	80	9.749783E-10	25.4375	24
GCCGTCT	1475	0.0	25.210169	36
ATATAGC	60	1.3342742E-6	24.666668	6
TCCGCCG	55	1.899202E-5	23.545454	23
CGGTGTA	55	1.899202E-5	23.545454	34
TCGGTGT	55	1.899202E-5	23.545454	33
ACGGTCA	40	0.0019293039	23.125	11
GTATCAA	1405	0.0	22.25267	1
TGATACA	50	2.6984318E-4	22.2	2
GGTGTAC	60	3.719562E-5	21.583334	35
GCCGTAG	60	3.719562E-5	21.583334	26
>>END_MODULE