FastQCFastQC Report
Fri 10 Feb 2017
ERR1631208.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631208.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1641090
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT102990.6275706999616109No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT93610.5704135665929352No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT93220.5680370972950904No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG88800.5411037785861836No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT58820.35842031820314546TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC52600.32051867965803216No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA41220.2511745242491271No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT31380.19121437581119866No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA27060.16489040820430323No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC27020.16464666776349865No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA22840.1391757916994193No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT22720.13844457037700553No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT19030.11595951471278236No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT17770.10828169082743785No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG17430.10620989708059889No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC17330.1056005459785874RNA PCR Primer, Index 7 (95% over 22bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC17310.1054786757581851No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG1600.033.531251
CTTATAC36950.032.543981
TTATACA37900.031.5329822
CGTCTTC10000.030.52537
TATACAC40700.029.6363643
GGTATCA11850.029.350211
CCGTCTT32950.025.7147237
GCCGTCT33700.025.14243536
GTATCAA37950.024.6666661
TAACGCA502.702785E-422.215
CGCCCGA1800.021.58333211
TGCCGTC42500.019.9835
TGTTACG851.2459732E-619.58823416
TCGCCCG1900.019.47368410
GCTCTAG1052.2611857E-819.380951
CGCCGTT1052.2611857E-819.3809525
TTACGGG2800.019.1607153
CGGATCG1457.2759576E-1219.13793237
ATGCCGT44600.018.99775734
CCGTTTA1205.178663E-918.527
Produced by FastQC (version 0.11.5)