##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631208.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1641090
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.434979190659867	31.0	31.0	34.0	28.0	34.0
2	31.431415705415304	31.0	31.0	34.0	28.0	34.0
3	30.891843226148474	31.0	31.0	34.0	27.0	34.0
4	35.038265421153014	37.0	35.0	37.0	32.0	37.0
5	35.24889737918091	37.0	35.0	37.0	33.0	37.0
6	35.44950612093182	37.0	35.0	37.0	33.0	37.0
7	35.46192408703971	37.0	35.0	37.0	33.0	37.0
8	35.483598096387155	37.0	35.0	37.0	33.0	37.0
9	37.255829357317396	39.0	37.0	39.0	34.0	39.0
10	37.09048254513768	39.0	37.0	39.0	33.0	39.0
11	37.145035311896365	39.0	37.0	39.0	33.0	39.0
12	37.07277480211323	39.0	37.0	39.0	33.0	39.0
13	37.152841099513125	39.0	37.0	39.0	33.0	39.0
14	38.23999354087832	40.0	38.0	41.0	33.0	41.0
15	38.237302646411834	40.0	38.0	41.0	33.0	41.0
16	38.241836827961905	40.0	38.0	41.0	33.0	41.0
17	38.22343990884107	40.0	38.0	41.0	33.0	41.0
18	38.27082122248018	40.0	38.0	41.0	33.0	41.0
19	38.29793369041308	40.0	38.0	41.0	34.0	41.0
20	38.279925537295334	40.0	38.0	41.0	34.0	41.0
21	38.250840599845226	40.0	38.0	41.0	34.0	41.0
22	38.17054396772877	40.0	38.0	41.0	33.0	41.0
23	38.17557477042697	40.0	37.0	41.0	34.0	41.0
24	38.12827328178223	40.0	37.0	41.0	33.0	41.0
25	38.08943080513561	40.0	37.0	41.0	33.0	41.0
26	37.97911875643627	40.0	37.0	41.0	33.0	41.0
27	37.894899121925064	40.0	37.0	41.0	33.0	41.0
28	37.89283037493373	40.0	37.0	41.0	33.0	41.0
29	37.82224436197893	40.0	37.0	41.0	33.0	41.0
30	37.69922185864273	40.0	37.0	41.0	33.0	41.0
31	37.65489887818462	40.0	37.0	41.0	33.0	41.0
32	37.54071744998751	39.0	36.0	41.0	32.0	41.0
33	37.49741574197637	39.0	36.0	41.0	32.0	41.0
34	37.438873553552824	39.0	36.0	41.0	32.0	41.0
35	37.385954457098634	39.0	36.0	41.0	32.0	41.0
36	37.34151569993114	39.0	36.0	41.0	32.0	41.0
37	37.267425308788674	39.0	36.0	41.0	31.0	41.0
38	37.212949929619946	39.0	36.0	41.0	31.0	41.0
39	37.107872816237986	39.0	36.0	41.0	31.0	41.0
40	36.99053007452364	39.0	36.0	41.0	31.0	41.0
41	36.91686379174817	39.0	36.0	41.0	31.0	41.0
42	36.824652517534076	39.0	35.0	41.0	31.0	41.0
43	36.16240973986802	38.0	35.0	40.0	29.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	13.0
10	23.0
11	24.0
12	32.0
13	18.0
14	21.0
15	21.0
16	15.0
17	29.0
18	42.0
19	79.0
20	176.0
21	325.0
22	689.0
23	1276.0
24	2174.0
25	3889.0
26	6564.0
27	10044.0
28	14848.0
29	21364.0
30	28713.0
31	37625.0
32	49335.0
33	63535.0
34	84751.0
35	113002.0
36	156827.0
37	232756.0
38	388836.0
39	424040.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.98841014203974	22.32345575196973	15.447659787092723	24.240474318897807
2	14.238097849599962	21.940844195016727	40.77619143374221	23.044866521641104
3	14.519861799170064	29.494482325771287	33.320719765521694	22.664936109536953
4	11.4080885265281	19.14026653017202	38.19181153989117	31.25983340340871
5	12.441487060429349	38.44932331560121	35.13311274823441	13.97607687573503
6	27.133368675697252	40.519410879354574	17.37674350584063	14.970476939107543
7	24.75464477877508	32.42649702331987	22.994046639733348	19.824811558171703
8	25.273811917688853	33.963950788805	22.050039912497184	18.712197381008963
9	24.11537453765485	14.415419020285297	19.40966065237129	42.05954578968856
10	15.424809120767295	27.765996989805558	32.92525090031626	23.883942989110896
11	31.704111291885273	23.394999664856893	24.572631604604254	20.328257438653576
12	21.043209086643632	27.017165420543666	27.905294651725377	24.034330841087325
13	29.503074176309646	20.42191470303274	26.17687025086985	23.898140869787763
14	22.282629228134958	22.050222717827786	26.58135751238506	29.085790541652194
15	24.523578840892334	28.321298649068606	22.942922082274585	24.212200427764476
16	24.560261777233425	26.045189477725174	23.757076089672108	25.637472655369297
17	21.081598206070357	29.741939808298145	24.709004381234422	24.467457604397076
18	23.728924068759177	24.670371521366896	26.314157054153032	25.286547355720895
19	24.03189343667928	26.15091189392416	26.958972390301568	22.858222279094992
20	22.68985856960922	25.237616462229372	27.371076540591922	24.70144842756948
21	24.388181026025386	24.93361119743585	26.562955109104315	24.11525266743445
22	21.29907561437825	28.877087789213267	27.644736120505275	22.17910047590321
23	23.441797829491374	24.373130053805703	27.1837620118336	25.001310104869322
24	22.36391666514329	27.24487992736535	27.522134678780567	22.869068728710793
25	21.768946249139294	27.99462552328026	27.58617747960197	22.65025074797848
26	23.404810217599277	25.610965882431795	27.050314120493084	23.933909779475837
27	21.177449134416758	28.671005246512987	28.240254952501083	21.91129066656917
28	21.449829077015885	24.910455855559416	28.49569493446429	25.14402013296041
29	21.942976923873765	25.065657581241734	28.710369327702928	24.280996167181566
30	21.731471156365586	26.068893235593414	29.354209702088248	22.84542590595275
31	23.355757453887353	24.184109341961747	27.19820363295127	25.26192957119963
32	21.60143563119634	26.469541585165956	29.26573192207618	22.66329086156152
33	21.4716438464679	25.203127189855522	28.741080623244308	24.584148340432275
34	21.317478017658996	27.021979294249554	28.478876844048774	23.18166584404268
35	21.54507065426028	26.502873090445984	31.083730934927395	20.868325320366342
36	23.474337178338786	25.62327477469243	25.273446307027648	25.628941739941137
37	22.394445155354063	26.10673393902833	28.2677366872018	23.23108421841581
38	22.475793527472597	24.809547313066314	28.19534577628284	24.519313383178254
39	23.888025641494373	25.08290221742866	27.443772127061894	23.585300014015075
40	21.38944238280655	24.784015501892036	31.830246970001646	21.996295145299772
41	22.900815921125595	25.19605871707219	26.609997014179598	25.293128347622616
42	22.477865321219433	25.035007220810556	29.65023246744542	22.836894990524588
43	23.351309190842674	24.00691004149681	29.61799779414901	23.02378297351151
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	6.0
1	23.0
2	40.0
3	182.5
4	325.0
5	325.0
6	459.5
7	594.0
8	659.5
9	725.0
10	1134.5
11	1544.0
12	1544.0
13	2465.5
14	3387.0
15	8752.0
16	14117.0
17	14893.0
18	15669.0
19	15669.0
20	13486.0
21	11303.0
22	11385.0
23	11467.0
24	14295.5
25	17124.0
26	17124.0
27	21653.0
28	26182.0
29	32742.0
30	39302.0
31	46473.5
32	53645.0
33	53645.0
34	63817.0
35	73989.0
36	81118.5
37	88248.0
38	97124.5
39	106001.0
40	106001.0
41	112665.5
42	119330.0
43	125037.5
44	130745.0
45	133462.5
46	136180.0
47	136180.0
48	138166.5
49	140153.0
50	149943.0
51	159733.0
52	145329.5
53	130926.0
54	130926.0
55	129238.5
56	127551.0
57	111187.5
58	94824.0
59	73409.0
60	51994.0
61	51994.0
62	43295.5
63	34597.0
64	27156.5
65	19716.0
66	15890.5
67	12065.0
68	12065.0
69	9645.0
70	7225.0
71	5807.0
72	4389.0
73	4065.0
74	3741.0
75	3741.0
76	3170.5
77	2600.0
78	1871.0
79	1142.0
80	710.5
81	279.0
82	279.0
83	211.5
84	144.0
85	98.0
86	52.0
87	37.5
88	23.0
89	23.0
90	17.0
91	11.0
92	6.5
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1641090.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	18.713176213774606
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36857522405572	13.542459005075576
2	10.708952673828637	4.007970369006561
3	4.052529255643159	2.2750708221698184
4	2.207065630039323	1.652048322011653
5	1.454505239071136	1.3609206421298263
6	1.0310389047994366	1.157640762526142
7	0.7664710795687062	1.004017586231188
8	0.5914516648883673	0.885435138158911
9	0.5412119309427106	0.9115014809455338
>10	4.568574232930186	18.40044038405405
>50	0.8698927769323347	11.498485521457136
>100	0.766961050430493	27.488642540884772
>500	0.05601400930110343	7.150695155081214
>1k	0.013883814271213669	4.274416364439207
>5k	0.002393761081243736	3.46757111327827
>10k+	4.7875221624874725E-4	0.9226847925501331
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	10299	0.6275706999616109	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9361	0.5704135665929352	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9322	0.5680370972950904	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	8880	0.5411037785861836	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	5882	0.35842031820314546	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	5260	0.32051867965803216	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	4122	0.2511745242491271	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3138	0.19121437581119866	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	2706	0.16489040820430323	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	2702	0.16464666776349865	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	2284	0.1391757916994193	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	2272	0.13844457037700553	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	1903	0.11595951471278236	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	1777	0.10828169082743785	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	1743	0.10620989708059889	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	1733	0.1056005459785874	RNA PCR Primer, Index 7 (95% over 22bp)
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	1731	0.1054786757581851	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	6.09351102011468E-5	0.0	0.0	0.0	0.0
5	6.09351102011468E-5	0.0	0.0	0.0	0.0
6	6.09351102011468E-5	0.0	0.0	0.0	0.0
7	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
8	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
9	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
10	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
11	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
12	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
13	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
14	6.09351102011468E-5	0.0	0.0	6.09351102011468E-5	0.0
15	6.09351102011468E-5	0.0	0.0	1.218702204022936E-4	0.0
16	6.09351102011468E-5	0.0	0.0	1.828053306034404E-4	0.0
17	1.218702204022936E-4	0.0	0.0	2.437404408045872E-4	0.0
18	1.218702204022936E-4	0.0	0.0	3.04675551005734E-4	0.0
19	1.218702204022936E-4	0.0	0.0	3.04675551005734E-4	0.0
20	1.218702204022936E-4	0.0	0.0	4.265457714080276E-4	0.0
21	1.218702204022936E-4	0.0	0.0	8.530915428160552E-4	0.0
22	1.218702204022936E-4	0.0	0.0	0.00152337775502867	0.0
23	1.218702204022936E-4	0.0	0.0	0.0017061830856321103	0.0
24	1.218702204022936E-4	0.0	0.0	0.0020108586366378444	0.0
25	1.218702204022936E-4	0.0	0.0	0.002132728857040138	0.0
26	1.218702204022936E-4	0.0	0.0	0.0023155341876435784	0.0
27	1.828053306034404E-4	0.0	0.0	0.002985820399856193	0.0
28	1.828053306034404E-4	0.0	0.0	0.01102925494640757	0.0
29	1.828053306034404E-4	0.0	0.0	0.025470876064079363	0.0
30	1.828053306034404E-4	0.0	0.0	0.047894996618101385	0.0
31	1.828053306034404E-4	0.0	0.0	0.09914142429726584	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	160	0.0	33.53125	1
CTTATAC	3695	0.0	32.54398	1
TTATACA	3790	0.0	31.532982	2
CGTCTTC	1000	0.0	30.525	37
TATACAC	4070	0.0	29.636364	3
GGTATCA	1185	0.0	29.35021	1
CCGTCTT	3295	0.0	25.71472	37
GCCGTCT	3370	0.0	25.142435	36
GTATCAA	3795	0.0	24.666666	1
TAACGCA	50	2.702785E-4	22.2	15
CGCCCGA	180	0.0	21.583332	11
TGCCGTC	4250	0.0	19.98	35
TGTTACG	85	1.2459732E-6	19.588234	16
TCGCCCG	190	0.0	19.473684	10
GCTCTAG	105	2.2611857E-8	19.38095	1
CGCCGTT	105	2.2611857E-8	19.38095	25
TTACGGG	280	0.0	19.160715	3
CGGATCG	145	7.2759576E-12	19.137932	37
ATGCCGT	4460	0.0	18.997757	34
CCGTTTA	120	5.178663E-9	18.5	27
>>END_MODULE