FastQCFastQC Report
Fri 10 Feb 2017
ERR1631207.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631207.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272960
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG74972.746556271981243No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT60662.2223036342321216No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT57592.109832942555686RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT56542.0713657678780772No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT48691.7837778429073858No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC41661.5262309495896833No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC21910.8026817116060961No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC20920.7664126611957797RNA PCR Primer, Index 47 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20710.7587192262602579No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17050.6246336459554513No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA14620.535609613130129No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13730.5030041031652989No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT11740.43009964830011727No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG10630.38943434935521687No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8200.30041031652989447No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8180.2996776084407972No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7760.2842907385697538No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7570.2773300117233294No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.2670720984759672No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7280.26670574443141853No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG7010.25681418522860494No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7000.2564478311840563No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6530.23922919109026966No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG6400.23446658851113714No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC6310.2311694021101993No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA6230.22823856975381007No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT6140.22494138335287223No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5730.2099208675263775No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC5470.20039566236811251No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5380.19709847596717467No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5170.18940504103165298No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5150.18867233294255567No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG5070.18574150058616648No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4990.18281066822977726No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG4890.17914712778429073No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4880.17878077373974208No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4870.17841441969519345No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4850.17768171160609614No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4770.17475087924970692No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT4630.1696219226260258No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4510.16522567409144195No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4450.16302754982415008No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4440.1626611957796014No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4410.16156213364595545No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4320.15826494724501758No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4300.1575322391559203No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT4120.15093786635404455No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG4080.14947245017584992No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3810.13958089097303633No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3720.13628370457209849No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3700.13555099648300117No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3690.13518464243845252No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT3690.13518464243845252No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3620.13262016412661196No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3570.1307883939038687No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3560.13042203985932005No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3550.1300556858147714No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3470.12712485345838218No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3380.12382766705744432No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3350.12272860492379836No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3300.12089683470105511No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA3220.11796600234466588No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3170.11613423212192263No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3090.11320339976553341No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3070.11247069167643611No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3050.1117379835873388No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3000.10990621336459555No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2990.10953985932004691No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2920.10697538100820633No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT2820.1033118405627198No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2810.10294548651817116No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC2800.10257913247362252No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT2790.10221277842907385No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2790.10221277842907385No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2780.10184642438452521No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCAGG200.001839478437.07
CTGTACA200.001839478437.09
ACGTTTA200.001839478437.026
TCTCTGC200.001839478437.08
CTGCGAG200.001839478437.01
GTTTTTG200.001839478437.016
TGTACAT200.001839478437.010
TTCTCTG200.001839478437.07
TCACGTT200.001839478437.024
CTTATAC5650.035.362831
AGCAACG401.594155E-632.3752
CGTCTTC3400.029.92647237
TTATACA6700.029.8208962
AGTTTTT250.005489093329.615
CCTAGTT250.005489093329.62
TGGGTAG250.005489093329.613
CATCCTA250.005489093329.614
TTGGGTA250.005489093329.612
CTGTACT453.993551E-628.7777794
GGTATCA5400.027.4074061
Produced by FastQC (version 0.11.5)