##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631207.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	272960
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.617639947245017	31.0	30.0	33.0	27.0	34.0
2	30.537016412661195	31.0	30.0	34.0	27.0	34.0
3	29.876219958968345	31.0	30.0	34.0	25.0	34.0
4	34.1599391852286	35.0	35.0	37.0	30.0	37.0
5	34.307458968347014	35.0	35.0	37.0	30.0	37.0
6	34.57214976553341	35.0	35.0	37.0	31.0	37.0
7	34.612562280187575	35.0	35.0	37.0	31.0	37.0
8	34.65124194021102	35.0	35.0	37.0	31.0	37.0
9	36.298889947245016	38.0	35.0	39.0	32.0	39.0
10	36.04746483001173	37.0	35.0	39.0	31.0	39.0
11	36.15115035169988	37.0	35.0	39.0	32.0	39.0
12	35.99700322391559	37.0	35.0	39.0	31.0	39.0
13	36.10428634232122	37.0	35.0	39.0	32.0	39.0
14	36.76589243845252	38.0	36.0	40.0	31.0	41.0
15	36.77885404454865	38.0	36.0	40.0	31.0	41.0
16	36.85278062719812	38.0	36.0	40.0	31.0	41.0
17	36.897120457209844	38.0	36.0	40.0	31.0	41.0
18	37.037437719812424	38.0	36.0	40.0	31.0	41.0
19	36.99772494138335	39.0	36.0	40.0	31.0	41.0
20	37.0433067116061	39.0	36.0	40.0	31.0	41.0
21	36.91169035756155	38.0	36.0	40.0	31.0	41.0
22	36.70309202813599	38.0	35.0	40.0	31.0	41.0
23	36.75975600820633	38.0	35.0	40.0	31.0	41.0
24	36.55387968933177	38.0	35.0	40.0	30.0	41.0
25	36.52068801289566	38.0	35.0	40.0	30.0	41.0
26	36.31302754982415	38.0	35.0	40.0	30.0	41.0
27	36.06850454279015	38.0	35.0	40.0	30.0	41.0
28	36.09915738569754	38.0	35.0	40.0	30.0	41.0
29	35.924538393903866	38.0	35.0	40.0	30.0	41.0
30	35.67896395076202	38.0	34.0	40.0	29.0	41.0
31	35.538404894490036	38.0	34.0	40.0	29.0	41.0
32	35.22226699882766	37.0	34.0	40.0	27.0	41.0
33	35.153524325908556	37.0	33.0	40.0	27.0	41.0
34	34.97526011137163	37.0	33.0	40.0	27.0	41.0
35	34.83000805978898	37.0	33.0	40.0	26.0	41.0
36	34.7066126905041	37.0	33.0	40.0	25.0	41.0
37	34.534591148886285	37.0	33.0	40.0	25.0	41.0
38	34.355923944900354	37.0	33.0	40.0	24.0	41.0
39	34.159917203985934	37.0	33.0	40.0	24.0	41.0
40	33.8459407971864	37.0	33.0	39.0	23.0	40.0
41	33.67545794255569	36.0	32.0	39.0	22.0	40.0
42	33.40117233294256	36.0	32.0	39.0	21.0	40.0
43	32.45238862837046	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	3.0
11	9.0
12	6.0
13	4.0
14	5.0
15	5.0
16	8.0
17	11.0
18	22.0
19	61.0
20	124.0
21	243.0
22	467.0
23	809.0
24	1208.0
25	1987.0
26	2818.0
27	4225.0
28	5664.0
29	7441.0
30	9673.0
31	11787.0
32	14591.0
33	18396.0
34	22923.0
35	29364.0
36	38162.0
37	46602.0
38	41655.0
39	14683.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.977652403282534	21.81784876905041	17.335140679953106	20.86935814771395
2	15.662368112543962	24.726333528722158	38.27813599062134	21.333162368112543
3	17.804806565064478	30.15276963657679	32.96453692848769	19.077886869871044
4	11.656286635404454	18.57671453692849	33.68185814771395	36.085140679953106
5	13.712998241500587	38.9961899179367	29.318947831184055	17.971864009378663
6	26.048138921453695	39.34202813599062	15.895369284876907	18.71446365767878
7	23.46754103165299	33.70310668229777	18.29608733880422	24.53326494724502
8	28.58367526377491	28.501978311840563	21.94790445486518	20.96644196951934
9	24.84759671746776	11.699882766705745	17.30143610785463	46.151084407971865
10	17.208748534583822	26.895149472450175	28.115841148886282	27.78026084407972
11	34.026963657678785	22.229630715123093	20.987690504103167	22.75571512309496
12	22.721277842907387	27.50329718640094	25.459041617819462	24.316383352872215
13	35.31726260257913	19.87690504103165	23.14514947245018	21.66068288393904
14	23.75256447831184	22.569240914419694	28.41002344665885	25.268171160609615
15	27.482415005861665	25.802315357561547	23.66830304806565	23.046966588511136
16	23.077740328253224	24.18486225087925	22.678048065650643	30.05934935521688
17	20.82319753810082	27.486444900351696	23.841222157092616	27.849135404454866
18	24.863349941383355	19.930392731535758	27.71431711606096	27.491940211019926
19	26.96439038686987	25.11466881594373	28.32063305978898	19.600307737397422
20	24.873241500586165	19.981315943728017	30.588364595545137	24.55707796014068
21	29.20354630715123	21.21556271981243	28.275937866354045	21.304953106682298
22	21.62514654161782	27.289712778429077	28.71885990621336	22.366280773739742
23	27.190430832356387	20.87412075029308	28.916324736225086	23.01912368112544
24	22.476186987104338	25.522787221570926	28.376685228604927	23.624340562719812
25	22.94512016412661	25.87888335287222	29.979117819460726	21.196878663540446
26	26.99956037514654	24.946878663540446	27.683543376318877	20.370017584994137
27	21.748974208675264	25.764947245017584	32.384964830011725	20.101113716295426
28	24.662221570926143	22.169548651817117	30.670427901524032	22.49780187573271
29	21.089903282532237	24.39698124267292	27.512822391559205	27.00029308323564
30	23.75036635404455	22.85499706916764	33.80165592028136	19.592980656506448
31	27.674750879249704	19.46658851113716	27.165152403282534	25.693508206330595
32	21.17050117233294	24.97838511137163	30.3667203985932	23.484393317702228
33	24.650864595545137	21.95156799531067	32.2831184056272	21.114449003517
34	19.3885550996483	26.491793669402107	29.156652989449007	24.962998241500586
35	20.75285756154748	24.427022274325907	34.92343200468933	19.896688159437282
36	25.05641852286049	22.179073856975382	28.184715709261432	24.579791910902696
37	23.390240328253224	23.4510550996483	30.591661781946073	22.567042790152403
38	22.164786049237982	21.18515533411489	29.745750293083233	26.904308323563892
39	25.91295427901524	21.61488862837046	29.062866354044548	23.409290738569755
40	21.096864009378663	22.14097303634232	34.70728311840563	22.05487983587339
41	24.574296600234465	22.42379835873388	27.37140973036342	25.630495310668227
42	22.32048651817116	21.641632473622508	33.009598475967174	23.028282532239157
43	24.52667057444314	21.498021688159437	29.84466588511137	24.13064185228605
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	7.5
2	11.0
3	83.0
4	155.0
5	155.0
6	247.0
7	339.0
8	388.5
9	438.0
10	657.5
11	877.0
12	877.0
13	1457.5
14	2038.0
15	5471.5
16	8905.0
17	8878.5
18	8852.0
19	8852.0
20	7104.0
21	5356.0
22	4076.5
23	2797.0
24	2566.0
25	2335.0
26	2335.0
27	2307.0
28	2279.0
29	2268.0
30	2257.0
31	2596.0
32	2935.0
33	2935.0
34	3752.0
35	4569.0
36	4411.0
37	4253.0
38	4913.5
39	5574.0
40	5574.0
41	6911.0
42	8248.0
43	10179.0
44	12110.0
45	15131.0
46	18152.0
47	18152.0
48	22395.0
49	26638.0
50	26662.0
51	26686.0
52	33590.0
53	40494.0
54	40494.0
55	32300.0
56	24106.0
57	26694.0
58	29282.0
59	23154.5
60	17027.0
61	17027.0
62	12332.0
63	7637.0
64	5821.0
65	4005.0
66	3236.0
67	2467.0
68	2467.0
69	1910.5
70	1354.0
71	954.0
72	554.0
73	338.0
74	122.0
75	122.0
76	87.0
77	52.0
78	37.0
79	22.0
80	20.5
81	19.0
82	19.0
83	13.0
84	7.0
85	5.5
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	272960.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.090709261430245
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67556799172723	17.50805978898007
2	11.08458286444235	5.340709261430246
3	4.419234161622921	3.1938745603751464
4	2.3860214726725264	2.299237983587339
5	1.672800267648043	2.014947245017585
6	1.198333282642416	1.732121922626026
7	0.8394415888561089	1.4155920281359906
8	0.6204568265458195	1.1957796014067994
9	0.4623011648772773	1.0023446658851114
>10	3.786611514948751	18.307810668229777
>50	0.44709388971684055	7.613936107854631
>100	0.3573709662702637	16.566896248534587
>500	0.02889382280482983	4.53802754982415
>1k	0.015207275160436753	8.120603751465417
>5k	0.0060829100641747015	9.150058616647128
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG	7497	2.746556271981243	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6066	2.2223036342321216	No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT	5759	2.109832942555686	RNA PCR Primer, Index 47 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	5654	2.0713657678780772	No Hit
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT	4869	1.7837778429073858	No Hit
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC	4166	1.5262309495896833	No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC	2191	0.8026817116060961	No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC	2092	0.7664126611957797	RNA PCR Primer, Index 47 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	2071	0.7587192262602579	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1705	0.6246336459554513	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1462	0.535609613130129	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1373	0.5030041031652989	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1174	0.43009964830011727	No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG	1063	0.38943434935521687	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	820	0.30041031652989447	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	818	0.2996776084407972	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	776	0.2842907385697538	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	757	0.2773300117233294	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	729	0.2670720984759672	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	728	0.26670574443141853	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	701	0.25681418522860494	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	700	0.2564478311840563	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	653	0.23922919109026966	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	640	0.23446658851113714	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	631	0.2311694021101993	No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA	623	0.22823856975381007	No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT	614	0.22494138335287223	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	573	0.2099208675263775	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	547	0.20039566236811251	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	538	0.19709847596717467	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	517	0.18940504103165298	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	515	0.18867233294255567	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	507	0.18574150058616648	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	499	0.18281066822977726	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	489	0.17914712778429073	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	488	0.17878077373974208	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	487	0.17841441969519345	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	485	0.17768171160609614	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	477	0.17475087924970692	No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT	463	0.1696219226260258	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	451	0.16522567409144195	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	445	0.16302754982415008	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	444	0.1626611957796014	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	441	0.16156213364595545	No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA	432	0.15826494724501758	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	430	0.1575322391559203	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	412	0.15093786635404455	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	408	0.14947245017584992	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	381	0.13958089097303633	No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA	372	0.13628370457209849	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	370	0.13555099648300117	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	369	0.13518464243845252	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	369	0.13518464243845252	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	362	0.13262016412661196	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	357	0.1307883939038687	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	356	0.13042203985932005	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	355	0.1300556858147714	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	347	0.12712485345838218	No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA	338	0.12382766705744432	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	335	0.12272860492379836	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	330	0.12089683470105511	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	322	0.11796600234466588	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	317	0.11613423212192263	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	309	0.11320339976553341	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	307	0.11247069167643611	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	305	0.1117379835873388	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	300	0.10990621336459555	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	299	0.10953985932004691	No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG	292	0.10697538100820633	No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT	282	0.1033118405627198	No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC	281	0.10294548651817116	No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC	280	0.10257913247362252	No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT	279	0.10221277842907385	No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG	279	0.10221277842907385	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	278	0.10184642438452521	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	3.663540445486518E-4	0.0
11	0.0	0.0	0.0	3.663540445486518E-4	0.0
12	0.0	0.0	0.0	3.663540445486518E-4	0.0
13	0.0	0.0	0.0	3.663540445486518E-4	0.0
14	0.0	0.0	0.0	3.663540445486518E-4	0.0
15	0.0	0.0	0.0	3.663540445486518E-4	0.0
16	0.0	0.0	0.0	3.663540445486518E-4	0.0
17	0.0	0.0	0.0	3.663540445486518E-4	0.0
18	0.0	0.0	0.0	3.663540445486518E-4	0.0
19	0.0	0.0	0.0	3.663540445486518E-4	0.0
20	0.0	0.0	0.0	3.663540445486518E-4	0.0
21	0.0	0.0	0.0	7.327080890973036E-4	0.0
22	0.0	0.0	0.0	0.001831770222743259	0.0
23	0.0	0.0	0.0	0.0029308323563892145	0.0
24	0.0	0.0	0.0	0.0032971864009378664	0.0
25	0.0	0.0	0.0	0.003663540445486518	0.0
26	0.0	0.0	0.0	0.00402989449003517	0.0
27	0.0	0.0	0.0	0.004762602579132473	0.0
28	0.0	0.0	0.0	0.016852286049237983	0.0
29	0.0	0.0	0.0	0.04213071512309496	0.0
30	0.0	0.0	0.0	0.08426143024618991	0.0
31	0.0	0.0	0.0	0.1392145369284877	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCAGG	20	0.0018394784	37.0	7
CTGTACA	20	0.0018394784	37.0	9
ACGTTTA	20	0.0018394784	37.0	26
TCTCTGC	20	0.0018394784	37.0	8
CTGCGAG	20	0.0018394784	37.0	1
GTTTTTG	20	0.0018394784	37.0	16
TGTACAT	20	0.0018394784	37.0	10
TTCTCTG	20	0.0018394784	37.0	7
TCACGTT	20	0.0018394784	37.0	24
CTTATAC	565	0.0	35.36283	1
AGCAACG	40	1.594155E-6	32.375	2
CGTCTTC	340	0.0	29.926472	37
TTATACA	670	0.0	29.820896	2
AGTTTTT	25	0.0054890933	29.6	15
CCTAGTT	25	0.0054890933	29.6	2
TGGGTAG	25	0.0054890933	29.6	13
CATCCTA	25	0.0054890933	29.6	14
TTGGGTA	25	0.0054890933	29.6	12
CTGTACT	45	3.993551E-6	28.777779	4
GGTATCA	540	0.0	27.407406	1
>>END_MODULE