FastQCFastQC Report
Fri 10 Feb 2017
ERR1631206.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631206.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences548859
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT130922.3853120746858485No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG127362.3204502431407703No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT117952.1490036603207745TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC71591.3043422809865557No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC39570.7209501893929042RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC39220.7145733239320117No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG17130.3121020152716818No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT16150.29424679198118275No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT15830.2884165149883668No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12100.2204573487908552No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT10060.18328933296165317No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT9600.17490830978448016No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6320.11514797060811612No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5800.1056737704947901No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15100.034.182121
CGTCTTC4550.032.5274737
TTATACA17650.029.4532572
TATACAC18300.028.6092873
CCGTCTT16400.026.73475837
ATTACCG358.863466E-426.4285725
GCCGTCT17000.025.79117436
GGTATCA2250.024.6666661
GTATCAA6500.024.4769231
CGCGGCT502.6996102E-422.210
TGCCGTC20800.021.07932935
ATTACCC909.4556526E-820.5555573
ATGCCGT21750.020.32873534
TATGCCG23150.019.01943833
CCCTAGT609.2284346E-418.51
ATAGACC500.00703031418.53
ATAGAAC609.2284346E-418.53
GTATACC1005.866541E-616.6500021
GTCTTAT803.3785394E-416.18751
TAGGACC803.3785394E-416.18754
Produced by FastQC (version 0.11.5)