##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631206.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	548859
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.470718344784363	31.0	31.0	34.0	28.0	34.0
2	31.49349833017223	31.0	31.0	34.0	28.0	34.0
3	30.971823364470655	31.0	31.0	34.0	27.0	34.0
4	35.094392184513694	37.0	35.0	37.0	32.0	37.0
5	35.28394906524262	37.0	35.0	37.0	33.0	37.0
6	35.473549673049	37.0	35.0	37.0	33.0	37.0
7	35.471886222144484	37.0	35.0	37.0	33.0	37.0
8	35.50947328913254	37.0	35.0	37.0	33.0	37.0
9	37.28486004602275	39.0	37.0	39.0	34.0	39.0
10	37.116773160319866	39.0	37.0	39.0	33.0	39.0
11	37.17605250164432	39.0	37.0	39.0	33.0	39.0
12	37.10134843375074	39.0	37.0	39.0	33.0	39.0
13	37.193379356082346	39.0	37.0	39.0	33.0	39.0
14	38.300998981523485	40.0	38.0	41.0	33.0	41.0
15	38.30216685888361	40.0	38.0	41.0	33.0	41.0
16	38.30894637784932	40.0	38.0	41.0	33.0	41.0
17	38.257614432850694	40.0	38.0	41.0	33.0	41.0
18	38.32363138802498	40.0	38.0	41.0	34.0	41.0
19	38.319200377510434	40.0	38.0	41.0	34.0	41.0
20	38.30971706758931	40.0	38.0	41.0	34.0	41.0
21	38.28039077431544	40.0	38.0	41.0	34.0	41.0
22	38.19196186998847	40.0	38.0	41.0	33.0	41.0
23	38.21347194816884	40.0	38.0	41.0	34.0	41.0
24	38.151517967273925	40.0	37.0	41.0	33.0	41.0
25	38.131017255797936	40.0	37.0	41.0	33.0	41.0
26	38.02742052148184	40.0	37.0	41.0	33.0	41.0
27	37.93767616090836	40.0	37.0	41.0	33.0	41.0
28	37.96027759406332	40.0	37.0	41.0	33.0	41.0
29	37.87255378886016	40.0	37.0	41.0	33.0	41.0
30	37.81208652859842	40.0	37.0	41.0	33.0	41.0
31	37.785356530547915	40.0	37.0	41.0	33.0	41.0
32	37.68550028331502	40.0	37.0	41.0	33.0	41.0
33	37.667419136791054	40.0	37.0	41.0	33.0	41.0
34	37.58571509258298	40.0	37.0	41.0	32.0	41.0
35	37.61195316101221	40.0	37.0	41.0	33.0	41.0
36	37.5958725282814	40.0	37.0	41.0	33.0	41.0
37	37.535671274407456	40.0	37.0	41.0	32.0	41.0
38	37.499594613552844	40.0	37.0	41.0	32.0	41.0
39	37.42211752016456	39.0	37.0	41.0	32.0	41.0
40	37.30104453056249	39.0	36.0	41.0	31.0	41.0
41	37.27920285537816	39.0	36.0	41.0	31.0	41.0
42	37.22819157561414	39.0	36.0	41.0	31.0	41.0
43	36.57414199275224	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	13.0
11	12.0
12	8.0
13	9.0
14	7.0
15	10.0
16	11.0
17	13.0
18	17.0
19	25.0
20	44.0
21	77.0
22	213.0
23	343.0
24	646.0
25	1212.0
26	1819.0
27	3004.0
28	4549.0
29	6490.0
30	8973.0
31	11906.0
32	15899.0
33	20390.0
34	27049.0
35	36372.0
36	51285.0
37	76125.0
38	132993.0
39	149333.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.518958421015235	23.757467764945094	15.829930820119555	24.893642993920114
2	12.839180918960972	21.679884997786317	41.76118092260489	23.719753160647816
3	13.84545028868981	29.098183686520585	35.179344786183705	21.877021238605906
4	10.969301769671263	19.75370723628473	36.621427361125534	32.65556363291847
5	12.065393844320672	38.327876558460375	35.733585492813276	13.873144104405686
6	27.237960933500226	39.74317629846645	17.768133527918827	15.250729240114492
7	23.191384308173866	34.18236742041216	22.294250435904303	20.331997835509664
8	24.62745440996686	35.134888924113476	23.01028132908452	17.227375336835145
9	22.5664514930064	14.913848547623342	19.278539661370225	43.24116029800003
10	13.85474229264711	28.687149158527053	33.82836028925462	23.62974825957122
11	30.409996009904184	22.51634755009939	25.401423680763184	21.672232759233246
12	19.146265252095713	26.981610941972345	28.92783028063674	24.9442935252952
13	29.147376648647466	20.1164597829315	26.674428222913356	24.06173534550768
14	21.283972750742905	23.203955842939624	25.79861130089877	29.713460105418697
15	23.774229811299442	29.209141145540112	21.984334774504926	25.03229426865552
16	25.436951931188155	27.265108160748024	23.021759686914127	24.276180221149694
17	19.85245755285055	32.348563110015505	24.494815608380293	23.30416372875365
18	23.25515296278279	25.33200694531747	25.928881552457007	25.483958539442735
19	22.170357049806963	28.813411094652725	27.310110611286326	21.70612124425399
20	21.60682433922009	28.247692030193548	25.047963138073715	25.097520492512647
21	27.000741538355022	25.15418349703658	25.6894758034395	22.155599161168897
22	22.030976990447456	31.670975605756666	25.425291377202523	20.87275602659335
23	23.288312663179433	25.544994251711277	25.632994995071595	25.5336980900377
24	23.13417471518186	30.006249328151675	26.933693352937638	19.925882603728827
25	21.48693926855531	28.709012697250113	27.294623938024152	22.50942409617042
26	26.472737078193127	26.430285373839187	27.52710623311269	19.569871314855
27	21.339360382174657	31.99036546726937	26.56237758695767	20.107896563598302
28	22.881286450618465	25.11373595039892	27.85086880237001	24.154108796612608
29	22.8858413545191	26.954645910880572	28.52299042194808	21.636522312652247
30	20.644282046937374	27.18876797137334	29.392977066969838	22.773972914719444
31	25.378831357416022	27.077263923885734	24.990389152769655	22.55351556592859
32	24.37839226468	25.893171105876007	29.442170029096726	20.286266600347265
33	22.18912325387759	25.456993508350962	29.285845727226846	23.068037510544602
34	20.47593279876981	31.527222838652552	26.58296575258855	21.413878609989087
35	20.752688759772546	25.092783392455985	34.45147114286183	19.703056704909642
36	25.174042878043355	25.56594680965421	25.15400130088055	24.10600901142188
37	21.32423810122454	26.98561925740491	28.713567601150753	22.976575040219803
38	21.33462328211799	24.56332136304588	29.16869359890245	24.93336175593367
39	24.60795942127213	25.520579966803858	26.669873319012716	23.201587292911295
40	20.160369056533646	24.403353138055493	33.77078630395056	21.665491501460302
41	22.17983124992029	26.338094118890282	26.021255003561933	25.460819627627494
42	22.230117388983327	24.525971151060656	31.528680407900755	21.715231052055266
43	22.735165133486014	24.147914127307743	30.330194093565016	22.78672664564123
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	11.0
2	18.0
3	68.5
4	119.0
5	119.0
6	133.5
7	148.0
8	156.0
9	164.0
10	262.0
11	360.0
12	360.0
13	550.0
14	740.0
15	1814.5
16	2889.0
17	3348.0
18	3807.0
19	3807.0
20	4197.0
21	4587.0
22	5622.0
23	6657.0
24	8359.5
25	10062.0
26	10062.0
27	12279.0
28	14496.0
29	16856.0
30	19216.0
31	21730.0
32	24244.0
33	24244.0
34	26902.5
35	29561.0
36	30889.5
37	32218.0
38	34010.0
39	35802.0
40	35802.0
41	36592.5
42	37383.0
43	37253.0
44	37123.0
45	37186.5
46	37250.0
47	37250.0
48	37637.0
49	38024.0
50	48981.0
51	59938.0
52	45309.0
53	30680.0
54	30680.0
55	38930.0
56	47180.0
57	38762.0
58	30344.0
59	22853.5
60	15363.0
61	15363.0
62	13264.0
63	11165.0
64	9274.5
65	7384.0
66	6144.5
67	4905.0
68	4905.0
69	3987.5
70	3070.0
71	2430.0
72	1790.0
73	1387.0
74	984.0
75	984.0
76	767.5
77	551.0
78	435.5
79	320.0
80	256.5
81	193.0
82	193.0
83	126.0
84	59.0
85	45.0
86	31.0
87	25.5
88	20.0
89	20.0
90	14.0
91	8.0
92	5.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	548859.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.45128627955818
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13318231200775	49.65056516994324
2	11.289200918876215	13.648934332288748
3	2.945097108343998	5.3410472525280595
4	1.1968550794175965	2.8940571616406587
5	0.657604722035373	1.9876525655274808
6	0.3862355075528836	1.4009059943045896
7	0.26141467134562946	1.1061997194633884
8	0.17606839301400745	0.8514848664697223
9	0.14199201250801316	0.7725239817779258
>10	0.7584338082984522	8.298675799591502
>50	0.037650466425078855	1.5154645488758804
>100	0.012048148277757412	1.2507179276980462
>500	9.036111208317855E-4	0.3954810509477537
>1k	0.0021084259486074996	2.732315216630752
>5k	3.012037069439285E-4	1.3035215670971312
>10k+	9.036111208317855E-4	6.850452845215164
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	13092	2.3853120746858485	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	12736	2.3204502431407703	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	11795	2.1490036603207745	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	7159	1.3043422809865557	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3957	0.7209501893929042	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3922	0.7145733239320117	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1713	0.3121020152716818	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1615	0.29424679198118275	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1583	0.2884165149883668	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1210	0.2204573487908552	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	1006	0.18328933296165317	No Hit
ACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT	960	0.17490830978448016	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	632	0.11514797060811612	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	580	0.1056737704947901	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	1.8219615602550017E-4	0.0
11	0.0	0.0	0.0	1.8219615602550017E-4	0.0
12	0.0	0.0	0.0	1.8219615602550017E-4	0.0
13	0.0	0.0	0.0	1.8219615602550017E-4	0.0
14	0.0	0.0	0.0	1.8219615602550017E-4	0.0
15	0.0	0.0	0.0	1.8219615602550017E-4	0.0
16	0.0	0.0	0.0	1.8219615602550017E-4	0.0
17	0.0	0.0	0.0	3.6439231205100033E-4	0.0
18	0.0	0.0	0.0	3.6439231205100033E-4	0.0
19	0.0	0.0	0.0	3.6439231205100033E-4	0.0
20	0.0	0.0	0.0	3.6439231205100033E-4	1.8219615602550017E-4
21	0.0	0.0	0.0	3.6439231205100033E-4	3.6439231205100033E-4
22	0.0	0.0	0.0	9.109807801275008E-4	3.6439231205100033E-4
23	0.0	0.0	0.0	9.109807801275008E-4	3.6439231205100033E-4
24	0.0	0.0	0.0	0.0014575692482040013	3.6439231205100033E-4
25	0.0	0.0	0.0	0.0016397654042295015	3.6439231205100033E-4
26	0.0	0.0	0.0	0.0018219615602550017	3.6439231205100033E-4
27	0.0	0.0	0.0	0.0027329423403825025	5.465884680765005E-4
28	0.0	0.0	0.0	0.008198827021147508	5.465884680765005E-4
29	0.0	0.0	0.0	0.016944242510371518	5.465884680765005E-4
30	0.0	0.0	0.0	0.028422600339978028	5.465884680765005E-4
31	0.0	0.0	0.0	0.06267547767277205	5.465884680765005E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1510	0.0	34.18212	1
CGTCTTC	455	0.0	32.52747	37
TTATACA	1765	0.0	29.453257	2
TATACAC	1830	0.0	28.609287	3
CCGTCTT	1640	0.0	26.734758	37
ATTACCG	35	8.863466E-4	26.428572	5
GCCGTCT	1700	0.0	25.791174	36
GGTATCA	225	0.0	24.666666	1
GTATCAA	650	0.0	24.476923	1
CGCGGCT	50	2.6996102E-4	22.2	10
TGCCGTC	2080	0.0	21.079329	35
ATTACCC	90	9.4556526E-8	20.555557	3
ATGCCGT	2175	0.0	20.328735	34
TATGCCG	2315	0.0	19.019438	33
CCCTAGT	60	9.2284346E-4	18.5	1
ATAGACC	50	0.007030314	18.5	3
ATAGAAC	60	9.2284346E-4	18.5	3
GTATACC	100	5.866541E-6	16.650002	1
GTCTTAT	80	3.3785394E-4	16.1875	1
TAGGACC	80	3.3785394E-4	16.1875	4
>>END_MODULE