FastQCFastQC Report
Fri 10 Feb 2017
ERR1631205.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631205.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences925649
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT73020.7888519298351752No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG64250.6941075936991236No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT41540.44876621700018043Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC34730.37519621368358846No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32590.35207729927866827No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT29590.3196676061876586No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC18220.19683486937273198No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC11870.1282343523300949Illumina PCR Primer Index 11 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10920.11797128285127516No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG800.032.3751
CTTATAC10600.030.0188671
GGTATCA4850.028.989691
TATACAC11950.028.0209223
TTATACA12550.026.0916332
GCCGTCT6350.025.92913436
GTATCAA17050.025.6070371
CCGTCTT6500.025.33076937
CGTCTTC1750.024.31428537
ATTACGG1002.0008883E-1124.0500012
GCGTCAA400.00193090523.12526
ACGGGTA450.003825253320.55555716
TAAGACG450.003825253320.5555574
TTCGGGC555.142053E-420.18181811
TGCCGTC8400.019.82142835
TTACGGG1153.0486262E-919.3043483
ATGCCGT8650.019.03468334
GGCGTCA609.234962E-418.525
GCCGCGT609.234962E-418.536
CGCGTGT500.00703387318.58
Produced by FastQC (version 0.11.5)