##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631205.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	925649
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.51655973268485	31.0	31.0	34.0	28.0	34.0
2	31.50398477176554	31.0	31.0	34.0	28.0	34.0
3	30.97032136371346	31.0	31.0	34.0	27.0	34.0
4	35.11002874739777	37.0	35.0	37.0	32.0	37.0
5	35.321418809937676	37.0	35.0	37.0	33.0	37.0
6	35.521148945226535	37.0	35.0	37.0	33.0	37.0
7	35.53434509193009	37.0	35.0	37.0	33.0	37.0
8	35.55283698248472	37.0	35.0	37.0	33.0	37.0
9	37.312455369151806	39.0	37.0	39.0	34.0	39.0
10	37.14699416301428	39.0	37.0	39.0	33.0	39.0
11	37.21129931539925	39.0	37.0	39.0	33.0	39.0
12	37.149767352419765	39.0	37.0	39.0	33.0	39.0
13	37.21668148509856	39.0	37.0	39.0	33.0	39.0
14	38.380553535951535	40.0	38.0	41.0	34.0	41.0
15	38.38560188581201	40.0	38.0	41.0	34.0	41.0
16	38.38759508193711	40.0	38.0	41.0	34.0	41.0
17	38.36955476643955	40.0	38.0	41.0	34.0	41.0
18	38.40825518095952	40.0	38.0	41.0	34.0	41.0
19	38.44101705938212	40.0	38.0	41.0	34.0	41.0
20	38.43183647365254	40.0	38.0	41.0	34.0	41.0
21	38.394271478713854	40.0	38.0	41.0	34.0	41.0
22	38.335695279744264	40.0	38.0	41.0	34.0	41.0
23	38.32554456386816	40.0	38.0	41.0	34.0	41.0
24	38.30031361779681	40.0	38.0	41.0	34.0	41.0
25	38.269818257244374	40.0	38.0	41.0	34.0	41.0
26	38.178264115231585	40.0	38.0	41.0	34.0	41.0
27	38.1170897391992	40.0	38.0	41.0	33.0	41.0
28	38.08840067887504	40.0	37.0	41.0	33.0	41.0
29	38.01660888738604	40.0	37.0	41.0	33.0	41.0
30	37.93638409375476	40.0	37.0	41.0	33.0	41.0
31	37.90654016803346	40.0	37.0	41.0	33.0	41.0
32	37.8383598966779	40.0	37.0	41.0	33.0	41.0
33	37.822597982604634	40.0	37.0	41.0	33.0	41.0
34	37.761001200238965	40.0	37.0	41.0	33.0	41.0
35	37.71562006764983	40.0	37.0	41.0	33.0	41.0
36	37.68001910011246	40.0	37.0	41.0	33.0	41.0
37	37.63096702961922	40.0	37.0	41.0	33.0	41.0
38	37.57695303511374	40.0	37.0	41.0	32.0	41.0
39	37.5172165691315	40.0	37.0	41.0	32.0	41.0
40	37.45797381080734	40.0	37.0	41.0	32.0	41.0
41	37.39502338359357	40.0	37.0	41.0	32.0	41.0
42	37.33880228898859	39.0	36.0	41.0	32.0	41.0
43	36.73970046961645	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	10.0
10	7.0
11	6.0
12	6.0
13	4.0
14	7.0
15	4.0
16	8.0
17	7.0
18	12.0
19	24.0
20	49.0
21	164.0
22	308.0
23	606.0
24	1079.0
25	1856.0
26	3013.0
27	4832.0
28	7126.0
29	10292.0
30	14249.0
31	19195.0
32	25409.0
33	33228.0
34	43999.0
35	59987.0
36	83313.0
37	124575.0
38	220814.0
39	271460.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.170895231345796	22.34529503083782	15.096543074102604	23.38726666371378
2	13.996342025973126	22.943794029918468	40.70182110065478	22.35804284345362
3	14.865353930053402	28.32304685685395	34.06831315109723	22.74328606199542
4	11.799072866712978	19.036373398556037	40.0410954908394	29.12345824389158
5	12.186044602219633	38.72288524051773	36.23976258819488	12.851307569067757
6	27.063174054096102	41.198985792670875	18.175355885438215	13.562484267794812
7	24.43561220289764	33.65292891798079	23.748742773988845	18.162716105132723
8	24.4566785034068	35.79132046812561	21.978741401978503	17.77325962648909
9	24.741235608745864	15.57955553346895	20.78390405002328	38.8953048077619
10	14.237254077949633	29.292312744895742	34.62500364609048	21.845429531064152
11	31.772842621771318	23.923755116680297	25.46721273398448	18.836189527563903
12	21.11448291955158	26.70763972088772	30.816648643276228	21.361228716284465
13	27.991171599602012	22.139385447399608	27.30570659072715	22.56373636227123
14	21.279988418936334	22.17546823904093	28.408392381993608	28.136150960029127
15	22.901985525831066	29.50103116840185	24.511342852420302	23.085640453346787
16	22.793845183217396	28.865477086887147	25.146356772383484	23.19432095751197
17	21.045666337888335	29.571684299340244	26.771810913207922	22.610838449563495
18	22.953624970156074	27.36080306898187	27.729193247116346	21.956378713745707
19	23.61348632148903	27.449497595740937	27.967296459024965	20.96971962374507
20	23.152728518045176	27.312620658586575	27.29587565048955	22.238775172878704
21	23.190323762030747	26.886109097508882	27.40725696241232	22.51631017804805
22	21.856016697473883	29.000625507076656	27.56930542786737	21.574052367582098
23	22.051339114502365	26.78866395361525	28.162294779122536	22.997702152759846
24	22.473205286237008	27.627318778500275	28.22462942216758	21.674846513095137
25	22.42188993884291	28.01439854631723	27.353132774950335	22.210578739889524
26	22.606949286392574	27.10563075204532	28.127832472135765	22.15958748942634
27	22.20841809368346	29.015209868967613	26.97761246433583	21.798759573013097
28	21.427020393259216	27.019745065354144	28.079325964809556	23.47390857657708
29	22.6815996128122	27.156189873267294	28.451497273804648	21.710713240115854
30	22.069272478012724	28.038921880756096	27.934670701313348	21.95713493991783
31	22.211551030682255	26.715958208781082	27.94309722151701	23.12939353901965
32	23.161371102869445	26.98236588598918	28.021528678797253	21.834734332344116
33	21.271345834112065	26.793633439889202	28.84559914179133	23.089421584207404
34	21.81366803183496	27.667830894864036	28.411309254371798	22.107191818929206
35	22.20906628754528	27.348919514848504	29.24639901301681	21.195615184589407
36	22.432369072942336	27.173258978295227	27.167425233538843	23.22694671522359
37	22.365064943623338	27.100877330391977	28.286423903661106	22.247633822323582
38	22.209930546027707	26.5785411100752	28.456790857009512	22.754737486887578
39	22.77699214281007	26.97804459357705	27.75414871079642	22.490814552816456
40	21.359932328560827	26.942393931176937	29.75231432216747	21.945359418094764
41	22.2481739838751	26.78866395361525	27.67463693041315	23.288525132096506
42	22.284796937067938	26.767813717726696	28.74102386541767	22.206365479787696
43	22.490706520506155	25.959299907416312	28.967999749365042	22.581993822712494
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	20.5
2	23.0
3	101.5
4	180.0
5	180.0
6	232.0
7	284.0
8	318.0
9	352.0
10	512.5
11	673.0
12	673.0
13	1063.5
14	1454.0
15	3348.0
16	5242.0
17	6219.0
18	7196.0
19	7196.0
20	7762.5
21	8329.0
22	10837.0
23	13345.0
24	17051.5
25	20758.0
26	20758.0
27	24816.5
28	28875.0
29	33289.0
30	37703.0
31	42384.5
32	47066.0
33	47066.0
34	50793.5
35	54521.0
36	58060.5
37	61600.0
38	63349.5
39	65099.0
40	65099.0
41	65547.5
42	65996.0
43	65815.5
44	65635.0
45	64792.5
46	63950.0
47	63950.0
48	61869.0
49	59788.0
50	58889.0
51	57990.0
52	63547.0
53	69104.0
54	69104.0
55	59219.5
56	49335.0
57	48245.0
58	47155.0
59	40208.0
60	33261.0
61	33261.0
62	27634.5
63	22008.0
64	18317.5
65	14627.0
66	12179.5
67	9732.0
68	9732.0
69	7892.0
70	6052.0
71	5039.5
72	4027.0
73	3130.5
74	2234.0
75	2234.0
76	1643.0
77	1052.0
78	803.5
79	555.0
80	381.0
81	207.0
82	207.0
83	160.0
84	113.0
85	88.5
86	64.0
87	50.0
88	36.0
89	36.0
90	22.0
91	8.0
92	4.5
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	925649.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.60440382633424
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.90333525940684	48.817994695253255
2	11.221786337316704	13.377357690045299
3	3.0583360164252267	5.468708848788948
4	1.2262904807082984	2.9236925208210782
5	0.6240613996199884	1.8598403837688566
6	0.3804885970257469	1.3607277593062783
7	0.24551267814869251	1.0243545769001647
8	0.1841178393243038	0.8779387237374342
9	0.13790953149394522	0.7398013865999103
>10	0.8698489631110927	10.004434595692462
>50	0.09222260364380416	3.7713447624144116
>100	0.052822898074077036	5.70858971381933
>500	0.0016336978506478938	0.6383510665326277
>1k	0.0012706538838372508	1.941669193219413
>5k	3.630439668106431E-4	1.485194083100573
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGT	7302	0.7888519298351752	No Hit
ATACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATG	6425	0.6941075936991236	No Hit
TCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTT	4154	0.44876621700018043	Illumina PCR Primer Index 11 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCC	3473	0.37519621368358846	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3259	0.35207729927866827	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2959	0.3196676061876586	No Hit
CATCTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTC	1822	0.19683486937273198	No Hit
CTCCGAGCCCACGAGACTACGCTGCATCTCGTATGCCGTCTTC	1187	0.1282343523300949	Illumina PCR Primer Index 11 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1092	0.11797128285127516	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.0803231030336553E-4	0.0
3	0.0	0.0	0.0	1.0803231030336553E-4	0.0
4	0.0	0.0	0.0	1.0803231030336553E-4	0.0
5	0.0	0.0	0.0	2.1606462060673107E-4	0.0
6	0.0	0.0	0.0	2.1606462060673107E-4	0.0
7	0.0	0.0	0.0	2.1606462060673107E-4	0.0
8	0.0	0.0	0.0	2.1606462060673107E-4	0.0
9	0.0	0.0	0.0	2.1606462060673107E-4	0.0
10	0.0	0.0	0.0	2.1606462060673107E-4	0.0
11	0.0	0.0	0.0	2.1606462060673107E-4	0.0
12	0.0	0.0	0.0	2.1606462060673107E-4	0.0
13	0.0	0.0	0.0	2.1606462060673107E-4	0.0
14	0.0	0.0	0.0	2.1606462060673107E-4	0.0
15	0.0	0.0	0.0	2.1606462060673107E-4	0.0
16	0.0	0.0	0.0	2.1606462060673107E-4	0.0
17	0.0	0.0	0.0	2.1606462060673107E-4	0.0
18	0.0	0.0	0.0	2.1606462060673107E-4	0.0
19	0.0	0.0	0.0	2.1606462060673107E-4	0.0
20	0.0	0.0	0.0	4.3212924121346214E-4	0.0
21	0.0	0.0	0.0	9.722907927302897E-4	0.0
22	0.0	0.0	0.0	0.0015124523442471174	0.0
23	0.0	0.0	0.0	0.0020526138957639452	0.0
24	0.0	0.0	0.0	0.002484743136977407	0.0
25	0.0	0.0	0.0	0.002700807757584138	0.0
26	0.0	0.0	0.0	0.002700807757584138	0.0
27	0.0	0.0	0.0	0.0035650662400110627	0.0
28	0.0	0.0	0.0	0.009722907927302897	0.0
29	0.0	0.0	0.0	0.022146623612189935	0.0
30	0.0	0.0	0.0	0.03770327629587457	0.0
31	0.0	0.0	0.0	0.0796198126935804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	80	0.0	32.375	1
CTTATAC	1060	0.0	30.018867	1
GGTATCA	485	0.0	28.98969	1
TATACAC	1195	0.0	28.020922	3
TTATACA	1255	0.0	26.091633	2
GCCGTCT	635	0.0	25.929134	36
GTATCAA	1705	0.0	25.607037	1
CCGTCTT	650	0.0	25.330769	37
CGTCTTC	175	0.0	24.314285	37
ATTACGG	100	2.0008883E-11	24.050001	2
GCGTCAA	40	0.001930905	23.125	26
ACGGGTA	45	0.0038252533	20.555557	16
TAAGACG	45	0.0038252533	20.555557	4
TTCGGGC	55	5.142053E-4	20.181818	11
TGCCGTC	840	0.0	19.821428	35
TTACGGG	115	3.0486262E-9	19.304348	3
ATGCCGT	865	0.0	19.034683	34
GGCGTCA	60	9.234962E-4	18.5	25
GCCGCGT	60	9.234962E-4	18.5	36
CGCGTGT	50	0.007033873	18.5	8
>>END_MODULE