FastQCFastQC Report
Fri 10 Feb 2017
ERR1631204.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631204.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119605
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT77006.437857949082396RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG72376.05075038668952No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT58934.927051544667865No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC42173.525772333932528No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC26592.2231512060532586No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC23521.9664729735378954RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT21721.8159775929099953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18201.5216755152376573No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG11070.9255465908615861No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA10490.8770536348814849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT6720.5618494210108272No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA6520.5451277120521718No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT6050.5058316959993312No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6040.5049956105513983No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA5940.4966347560720705No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT5740.47991304711341504No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT5040.42138706575812046No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC4450.37205802433008656No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4420.3695497679862882No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4400.3678775970904226No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4000.33443417917311147No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG3650.30517118849546426No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG3470.29012165043267424No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT2930.24497303624430417No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG2820.2357760963170436No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC2740.22908741273358138No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG2630.2198904728063208No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG2590.2165461310145897No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT2500.20902136198319465No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2370.19815225116006857No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2280.19062748212867356No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2280.19062748212867356No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2210.1847748839931441No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG2210.1847748839931441No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2170.18143054220141297No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2120.17725011496174908No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2100.17557794406588353No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG2090.17474185861795077No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2000.16721708958655573No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG1950.16303666234689185No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1930.1613644914510263No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT1930.1613644914510263No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1900.15885623510722796No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC1900.15885623510722796No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC1890.15802014965929517No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT1860.15551189331549686No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1830.15300363697169853No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT1770.14798712428410182No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1680.1404623552527068No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG1680.1404623552527068No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG1660.13879018435684126No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT1610.13460975711717738No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC1590.13293758622131183No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA1540.1287571589816479No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA1490.12457673174198403No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1480.12374064629405125No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA1470.12290456084611846No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1450.1212323899502529No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA1440.12039630450232013No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG1380.11537979181472346No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1380.11537979181472346No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG1380.11537979181472346No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG1370.11454370636679069No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC1350.11287153547092513No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1320.11036327912712679No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC1310.10952719367919402No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC1250.10451068099159733No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA1250.10451068099159733No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA1230.10283851009573178No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG1200.10033025375193345No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTTT200.001835351237.03
ACAGACA200.001835351237.017
GACAGAC200.001835351237.016
GTATCTT200.001835351237.036
ACTCAGA200.001835351237.01
CTGGTAT200.001835351237.033
TATCTTC200.001835351237.037
TCAGAGT200.001835351237.03
CAGACAC200.001835351237.018
AGACAGA200.001835351237.015
CTTATAC6800.035.6397061
CGTCTTC2800.035.0178637
TTATACA7500.032.562
TATACAC7800.031.5448723
ATACGGA303.580397E-430.8333344
TAATACG303.580397E-430.8333342
AATACGG303.580397E-430.8333343
GTAATAC303.580397E-430.8333341
CAGCTGG303.580397E-430.83333430
GTGTTTT250.00547691829.66
Produced by FastQC (version 0.11.5)