##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631204.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	119605
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.21764976380586	31.0	31.0	34.0	28.0	34.0
2	31.270933489402616	31.0	31.0	34.0	28.0	34.0
3	30.706659420592786	31.0	31.0	34.0	26.0	34.0
4	34.82108607499686	37.0	35.0	37.0	32.0	37.0
5	35.0721541741566	35.0	35.0	37.0	32.0	37.0
6	35.28769700263367	37.0	35.0	37.0	32.0	37.0
7	35.264336775218425	37.0	35.0	37.0	32.0	37.0
8	35.30374984323398	37.0	35.0	37.0	32.0	37.0
9	37.102504075916556	39.0	37.0	39.0	33.0	39.0
10	36.95349692738598	39.0	37.0	39.0	33.0	39.0
11	36.965085071694325	39.0	37.0	39.0	33.0	39.0
12	36.83980602817608	39.0	37.0	39.0	32.0	39.0
13	36.97051962710589	39.0	37.0	39.0	33.0	39.0
14	37.78984992266209	39.0	37.0	41.0	32.0	41.0
15	37.8176330420969	39.0	37.0	41.0	33.0	41.0
16	37.855691651686804	39.0	37.0	41.0	33.0	41.0
17	37.88925212156683	40.0	37.0	41.0	33.0	41.0
18	38.03310898373814	40.0	37.0	41.0	33.0	41.0
19	37.97725011496175	40.0	37.0	41.0	33.0	41.0
20	37.983127795660714	40.0	37.0	41.0	33.0	41.0
21	37.89201956439948	40.0	37.0	41.0	33.0	41.0
22	37.73260315204214	39.0	37.0	41.0	32.0	41.0
23	37.781664646126835	39.0	37.0	41.0	33.0	41.0
24	37.6529827348355	39.0	37.0	41.0	32.0	41.0
25	37.55932444295807	39.0	36.0	41.0	32.0	41.0
26	37.30342376990929	39.0	36.0	41.0	31.0	41.0
27	37.16543622758246	39.0	36.0	40.0	31.0	41.0
28	37.1338321976506	39.0	36.0	40.0	31.0	41.0
29	37.08712010367459	39.0	36.0	40.0	31.0	41.0
30	36.82307595836294	39.0	35.0	40.0	31.0	41.0
31	36.60723213912462	38.0	35.0	40.0	30.0	41.0
32	36.385719660549306	38.0	35.0	40.0	30.0	41.0
33	36.02688850800552	38.0	35.0	40.0	30.0	41.0
34	35.89812298816939	38.0	35.0	40.0	29.0	41.0
35	35.900957317837886	38.0	35.0	40.0	28.0	41.0
36	35.73284561682204	38.0	35.0	40.0	28.0	41.0
37	35.664763178796875	38.0	34.0	40.0	28.0	41.0
38	35.543631119100375	38.0	34.0	40.0	27.0	41.0
39	35.164725554951715	38.0	34.0	40.0	26.0	41.0
40	34.65014840516701	38.0	33.0	40.0	24.0	41.0
41	34.63504034112286	38.0	33.0	40.0	24.0	41.0
42	34.398595376447474	37.0	33.0	40.0	23.0	41.0
43	33.349032231094014	36.0	31.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	7.0
11	11.0
12	6.0
13	7.0
14	10.0
15	6.0
16	5.0
17	8.0
18	6.0
19	18.0
20	24.0
21	62.0
22	131.0
23	218.0
24	356.0
25	574.0
26	895.0
27	1337.0
28	1799.0
29	2423.0
30	2982.0
31	3762.0
32	4714.0
33	6056.0
34	7669.0
35	9912.0
36	13129.0
37	19018.0
38	25340.0
39	19110.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.520421387065756	24.310856569541407	19.325279043518247	24.843442999874586
2	11.446845867647673	21.76915680782576	41.751599013419174	25.032398311107396
3	12.999456544458843	32.08812340621211	37.173195100539274	17.73922494878977
4	8.727895990970277	19.325279043518247	27.28063208059864	44.66619288491284
5	13.022866937000963	37.444922871117434	29.043100204840933	20.489109987040678
6	26.373479369591575	35.46172818862088	15.766899377116342	22.39789306467121
7	20.630408427741315	35.6740938923958	15.451695163245684	28.2438025166172
8	29.461979014255256	26.52397475021947	25.054136532753645	18.959909702771625
9	19.48497136407341	11.55386480498307	14.450900882070147	54.510262948873375
10	16.05534885665315	25.134400735755197	26.96041135404038	31.84983905355127
11	29.882529994565445	19.428953639061913	20.82438025166172	29.86413611471092
12	18.518456586263117	29.271351532126584	20.108691108231262	32.10150077337904
13	36.173236904811674	15.696668199489988	22.38367961205635	25.746415283641987
14	24.310856569541407	25.702938840349482	22.035031980268382	27.951172609840725
15	28.49462815099703	26.079177291919237	17.842063458885498	27.584131098198235
16	29.531374106433677	21.613644914510264	17.10212783746499	31.75285314159107
17	18.16479244178755	34.987667739642994	18.209104970527985	28.63843484804147
18	25.835040341122863	15.26106768111701	23.810877471677607	35.09301450608252
19	23.897830358262613	27.150202750721125	28.528907654362275	20.423059236653987
20	19.519250867438654	28.07909368337444	22.119476610509594	30.282178838677314
21	30.69938547719577	24.460515864721373	24.14865599264245	20.691442665440405
22	26.07750512102337	35.13398269303123	21.97901425525689	16.809497930688515
23	31.04385268174407	19.477446595042014	23.269094101417164	26.20960662179675
24	26.38518456586263	24.238953221019187	25.881861126207102	23.49400108691108
25	27.20287613394089	30.02884494795368	21.56180761673843	21.206471301367
26	24.02324317545253	30.232013711801347	28.062371974415782	17.68237113833034
27	24.498975795326285	35.282805902763265	24.781572676727563	15.436645625182893
28	18.93817148112537	27.497178211613228	29.774674971782115	23.789975335479284
29	24.473057146440365	20.939760043476443	27.742987333305464	26.844195476777728
30	26.27398520128757	24.865181221520842	31.51456878893023	17.346264788261358
31	22.57179883784123	25.2263701350278	20.77337903933782	31.428451987793153
32	34.38234187533966	20.11956021905439	27.156055348856654	18.3420425567493
33	21.961456460850297	25.233058818611266	33.263659546005606	19.541825174532836
34	17.518498390535512	30.146732996112203	25.101793403285814	27.232975210066467
35	23.377785209648426	19.52677563647005	40.82521633710965	16.270222816771874
36	24.25149450273818	25.019857029388405	22.833493583044188	27.895154884829232
37	21.310145896910665	23.004891099870406	30.666778144726393	25.018184858492535
38	20.754149074035368	17.27937795242674	30.03553363153714	31.93093934200075
39	30.57982525814138	18.857907278123825	25.853434220977384	24.70883324275741
40	17.709125872664185	17.992558839513396	42.45474687513064	21.843568412691777
41	24.686258935663226	21.33690063124451	23.502361941390408	30.474478491701852
42	22.135362234020317	17.25596755988462	38.74921616989256	21.8594540362025
43	24.478073659127965	17.446595042013293	33.11901676351323	24.956314535345513
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.5
2	2.0
3	27.0
4	52.0
5	52.0
6	80.5
7	109.0
8	118.5
9	128.0
10	212.5
11	297.0
12	297.0
13	469.5
14	642.0
15	1846.5
16	3051.0
17	2966.5
18	2882.0
19	2882.0
20	2331.0
21	1780.0
22	1381.5
23	983.0
24	966.5
25	950.0
26	950.0
27	972.5
28	995.0
29	915.5
30	836.0
31	870.0
32	904.0
33	904.0
34	1045.0
35	1186.0
36	1145.5
37	1105.0
38	1243.5
39	1382.0
40	1382.0
41	1747.5
42	2113.0
43	2934.5
44	3756.0
45	5071.0
46	6386.0
47	6386.0
48	8884.0
49	11382.0
50	17089.5
51	22797.0
52	16746.0
53	10695.0
54	10695.0
55	16330.5
56	21966.0
57	17754.5
58	13543.0
59	8821.5
60	4100.0
61	4100.0
62	3322.0
63	2544.0
64	1986.5
65	1429.0
66	1149.5
67	870.0
68	870.0
69	679.5
70	489.0
71	341.0
72	193.0
73	109.5
74	26.0
75	26.0
76	19.5
77	13.0
78	10.5
79	8.0
80	7.5
81	7.0
82	7.0
83	4.5
84	2.0
85	1.5
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	119605.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	21.187241336064545
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.36888836273233	15.332971029639229
2	10.765163174302513	4.56168220392124
3	4.506530918274733	2.8644287446176997
4	2.5373900003946175	2.150411772083107
5	1.5271694092577246	1.6178253417499269
6	1.1522828617655183	1.4648217047782284
7	0.9194585849019375	1.363655365578362
8	0.6629572629335859	1.1236988420216545
9	0.5485182115938597	1.045942895363906
>10	4.234244899569867	17.745913632373227
>50	0.4261868118858767	5.9387149366665275
>100	0.2841245412572511	11.002884494795367
>500	0.027623219288899412	3.5157393085573343
>1k	0.027623219288899412	12.855649847414405
>5k	0.011838522552385463	17.415659880439783
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTT	7700	6.437857949082396	RNA PCR Primer, Index 46 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATG	7237	6.05075038668952	No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGT	5893	4.927051544667865	No Hit
ACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCC	4217	3.525772333932528	No Hit
CATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTC	2659	2.2231512060532586	No Hit
CTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCTTC	2352	1.9664729735378954	RNA PCR Primer, Index 46 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2172	1.8159775929099953	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	1.5216755152376573	No Hit
CACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCG	1107	0.9255465908615861	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1049	0.8770536348814849	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	672	0.5618494210108272	No Hit
TTATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	652	0.5451277120521718	No Hit
ACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGT	605	0.5058316959993312	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	604	0.5049956105513983	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	594	0.4966347560720705	No Hit
TATACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTAT	574	0.47991304711341504	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	504	0.42138706575812046	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	445	0.37205802433008656	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	442	0.3695497679862882	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	440	0.3678775970904226	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	400	0.33443417917311147	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	365	0.30517118849546426	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	347	0.29012165043267424	No Hit
ATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTATGCCGTCT	293	0.24497303624430417	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	282	0.2357760963170436	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	274	0.22908741273358138	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	263	0.2198904728063208	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	259	0.2165461310145897	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	250	0.20902136198319465	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	237	0.19815225116006857	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	228	0.19062748212867356	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	228	0.19062748212867356	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	221	0.1847748839931441	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	221	0.1847748839931441	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	217	0.18143054220141297	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	212	0.17725011496174908	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	210	0.17557794406588353	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	209	0.17474185861795077	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	200	0.16721708958655573	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	195	0.16303666234689185	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	193	0.1613644914510263	No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT	193	0.1613644914510263	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	190	0.15885623510722796	No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC	190	0.15885623510722796	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	189	0.15802014965929517	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	186	0.15551189331549686	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	183	0.15300363697169853	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	177	0.14798712428410182	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	168	0.1404623552527068	No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG	168	0.1404623552527068	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	166	0.13879018435684126	No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT	161	0.13460975711717738	No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC	159	0.13293758622131183	No Hit
CTTTACACATCTCCGAGCCCACGAGACTAAGGCGAATCTCGTA	154	0.1287571589816479	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	149	0.12457673174198403	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	148	0.12374064629405125	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	147	0.12290456084611846	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	145	0.1212323899502529	No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA	144	0.12039630450232013	No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG	138	0.11537979181472346	No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	138	0.11537979181472346	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	138	0.11537979181472346	No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG	137	0.11454370636679069	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	135	0.11287153547092513	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	132	0.11036327912712679	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC	131	0.10952719367919402	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	125	0.10451068099159733	No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA	125	0.10451068099159733	No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA	123	0.10283851009573178	No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG	120	0.10033025375193345	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	8.360854479327787E-4	0.0
9	0.0	0.0	0.0	8.360854479327787E-4	0.0
10	0.0	0.0	0.0	0.0016721708958655575	0.0
11	0.0	0.0	0.0	0.002508256343798336	0.0
12	0.0	0.0	0.0	0.002508256343798336	0.0
13	0.0	0.0	0.0	0.002508256343798336	0.0
14	0.0	0.0	0.0	0.002508256343798336	0.0
15	0.0	0.0	0.0	0.002508256343798336	0.0
16	0.0	0.0	0.0	0.002508256343798336	0.0
17	0.0	0.0	0.0	0.002508256343798336	0.0
18	0.0	0.0	0.0	0.002508256343798336	0.0
19	0.0	0.0	0.0	0.002508256343798336	0.0
20	0.0	0.0	0.0	0.002508256343798336	0.0
21	0.0	0.0	0.0	0.004180427239663893	0.0
22	0.0	0.0	0.0	0.005016512687596672	0.0
23	0.0	0.0	0.0	0.007524769031395009	0.0
24	0.0	0.0	0.0	0.007524769031395009	0.0
25	0.0	0.0	0.0	0.008360854479327787	0.0
26	0.0	0.0	0.0	0.008360854479327787	0.0
27	0.0	0.0	0.0	0.012541281718991682	0.0
28	0.0	0.0	0.0	0.03260733246937837	0.0
29	0.0	0.0	0.0	0.05768989590736173	0.0
30	0.0	0.0	0.0	0.09614982651226955	0.0
31	0.0	0.0	0.0	0.14046235525270684	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTACTTT	20	0.0018353512	37.0	3
ACAGACA	20	0.0018353512	37.0	17
GACAGAC	20	0.0018353512	37.0	16
GTATCTT	20	0.0018353512	37.0	36
ACTCAGA	20	0.0018353512	37.0	1
CTGGTAT	20	0.0018353512	37.0	33
TATCTTC	20	0.0018353512	37.0	37
TCAGAGT	20	0.0018353512	37.0	3
CAGACAC	20	0.0018353512	37.0	18
AGACAGA	20	0.0018353512	37.0	15
CTTATAC	680	0.0	35.639706	1
CGTCTTC	280	0.0	35.01786	37
TTATACA	750	0.0	32.56	2
TATACAC	780	0.0	31.544872	3
ATACGGA	30	3.580397E-4	30.833334	4
TAATACG	30	3.580397E-4	30.833334	2
AATACGG	30	3.580397E-4	30.833334	3
GTAATAC	30	3.580397E-4	30.833334	1
CAGCTGG	30	3.580397E-4	30.833334	30
GTGTTTT	25	0.005476918	29.6	6
>>END_MODULE