FastQCFastQC Report
Fri 10 Feb 2017
ERR1631203.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631203.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences892274
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT180632.0243781618650774No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG131621.475107422159561No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT120251.347680196890193RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC66880.7495455431851651No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC47960.537503053994625RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC33790.37869533349621304RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT20950.234793348231597No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19830.22224115013997942No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT18460.20688712211719718No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC18120.20307663341081328No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG16020.17954126198903028No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC15610.17494626090192028No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC15060.16878223505335804No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT14440.16183369682406973No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA14050.1574628421314529No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA13760.1542127194113019No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT13720.15376442662231557No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG13700.1535402802278224No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC11630.13034112839777914No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA11440.12821173765009403No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT10890.12204771180153182No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA10880.12193563860428525No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT10680.11969417465935353No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA10640.11924588187036717No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC10540.11812514989790132No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG10330.11577161275572302No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA9230.10344356105859859No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC8960.10041758473294078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21900.033.7899551
TATACAC24400.030.8586063
TTATACA24500.030.2040822
CGTCTTC6550.029.65648737
TTACCGG250.00549569329.5999984
TTACGGG556.259106E-726.9090943
ACACCGT358.867836E-426.428576
GGTATCA4650.025.8602141
GCCGTCT19950.024.85213336
CCGTCTT19950.024.75940137
GATTACG701.921926E-723.7857131
GTATCAA12900.023.232561
CCGGCGT400.001930850723.12523
GATACAC603.7250822E-521.5833343
TGCCGTC23650.021.04228435
GCGTCAA450.003825147620.55555526
ATGCCGT24600.020.45528434
CACGACA555.141853E-420.1818234
ATTACGG759.261965E-619.7333322
TTAACGG951.675071E-719.47368435
Produced by FastQC (version 0.11.5)