##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631203.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	892274
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47726258974261	31.0	31.0	34.0	28.0	34.0
2	31.49557198797679	31.0	31.0	34.0	28.0	34.0
3	30.97185169577955	31.0	31.0	34.0	27.0	34.0
4	35.108983339198495	37.0	35.0	37.0	32.0	37.0
5	35.307163494621605	37.0	35.0	37.0	33.0	37.0
6	35.4891199340113	37.0	35.0	37.0	33.0	37.0
7	35.49125156622293	37.0	35.0	37.0	33.0	37.0
8	35.515221781650034	37.0	35.0	37.0	33.0	37.0
9	37.28898185983229	39.0	37.0	39.0	34.0	39.0
10	37.13107857003566	39.0	37.0	39.0	33.0	39.0
11	37.18949896556439	39.0	37.0	39.0	33.0	39.0
12	37.124815919773525	39.0	37.0	39.0	33.0	39.0
13	37.19324221035242	39.0	37.0	39.0	33.0	39.0
14	38.32874767167933	40.0	38.0	41.0	34.0	41.0
15	38.33566595014536	40.0	38.0	41.0	34.0	41.0
16	38.33508316951968	40.0	38.0	41.0	34.0	41.0
17	38.28672470563975	40.0	38.0	41.0	33.0	41.0
18	38.340303539047426	40.0	38.0	41.0	34.0	41.0
19	38.362838096817796	40.0	38.0	41.0	34.0	41.0
20	38.3147060207963	40.0	38.0	41.0	34.0	41.0
21	38.232524986719326	40.0	38.0	41.0	34.0	41.0
22	38.217540800247455	40.0	38.0	41.0	34.0	41.0
23	38.21713845746934	40.0	38.0	41.0	34.0	41.0
24	38.189264732582146	40.0	38.0	41.0	33.0	41.0
25	38.15014558308322	40.0	38.0	41.0	33.0	41.0
26	38.02023257429892	40.0	37.0	41.0	33.0	41.0
27	37.95973434169325	40.0	37.0	41.0	33.0	41.0
28	37.88955186411349	40.0	37.0	41.0	33.0	41.0
29	37.905607470351036	40.0	37.0	41.0	33.0	41.0
30	37.83162907358054	40.0	37.0	41.0	33.0	41.0
31	37.708441577363004	40.0	37.0	41.0	32.0	41.0
32	37.712208357522464	40.0	37.0	41.0	33.0	41.0
33	37.72513936302077	40.0	37.0	41.0	33.0	41.0
34	37.675978455048565	40.0	37.0	41.0	33.0	41.0
35	37.67071325624192	40.0	37.0	41.0	33.0	41.0
36	37.64592490647492	40.0	37.0	41.0	33.0	41.0
37	37.57792673550949	40.0	37.0	41.0	32.0	41.0
38	37.52791070904229	40.0	37.0	41.0	32.0	41.0
39	37.44505723578183	40.0	37.0	41.0	32.0	41.0
40	37.36431298009356	39.0	37.0	41.0	31.0	41.0
41	37.335637931846044	39.0	37.0	41.0	31.0	41.0
42	37.2776904852097	39.0	36.0	41.0	31.0	41.0
43	36.61669397516906	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	12.0
10	19.0
11	11.0
12	15.0
13	15.0
14	16.0
15	14.0
16	12.0
17	14.0
18	15.0
19	46.0
20	64.0
21	161.0
22	292.0
23	594.0
24	1136.0
25	1980.0
26	3126.0
27	4920.0
28	7418.0
29	10473.0
30	14723.0
31	19560.0
32	25300.0
33	33085.0
34	43740.0
35	58592.0
36	81555.0
37	120484.0
38	211937.0
39	252941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.48781203979944	22.536014721935192	15.342708629860336	24.633464608405042
2	13.28773448514694	22.23890867603449	41.761275124008996	22.712081714809575
3	14.312980093558705	28.26093778368528	35.673683195968955	21.752398926787066
4	11.705148866827903	19.591515610675643	37.12368622194528	31.57964930055118
5	12.069835050668292	38.85734651015271	36.09362146605191	12.979196973127088
6	27.237933639218447	40.08051338490194	18.032913656567377	14.64863931931223
7	23.397409315972446	34.14791868865393	22.843991867968807	19.610680127404812
8	23.79818306932624	36.222729789279974	22.435821283596745	17.543265857797046
9	23.198255244465265	15.718826279819876	19.424302400383738	41.658616075331125
10	13.84541071464595	29.477492339796967	33.92063424463786	22.756462700919226
11	29.875240116825104	23.420384321407997	26.129529718449714	20.57484584331719
12	19.422285082833298	26.915835270331762	29.221965450074755	24.43991419676019
13	27.632431293526427	21.040622051074	28.001264185664944	23.32568246973463
14	21.105736578674264	23.0559222839621	26.26166401800344	29.576677119360195
15	23.645651447873636	29.086244808209138	23.385081264275325	23.883022479641905
16	24.76940939666515	28.238411071038716	23.34809710918395	23.644082423112184
17	20.892012991525025	31.303949235324573	24.82656672726091	22.97747104588949
18	23.2495847688042	26.458800771960185	26.147573503206413	24.144040956029205
19	24.997030060272966	27.59421433326534	26.121124228656218	21.28763137780547
20	23.498275193494376	26.32980452192936	26.41755783537344	23.754362449202823
21	23.022972763971605	27.873500740803834	25.27889415134813	23.82463234387643
22	20.34767347249836	31.036654659891465	26.072148241459463	22.543523626150712
23	21.931491895987108	26.74738925487014	27.234907662892788	24.086211186249965
24	24.32604782835766	27.914855750587826	25.162113879817188	22.596982541237335
25	22.220976964475035	27.825085119593307	26.57894323940852	23.37499467652313
26	24.397886747792718	26.978708333987093	26.92502527250598	21.69837964571421
27	22.290126127176183	29.094202005213642	26.301337929828726	22.314333937781445
28	19.97570253083694	27.764453519882903	28.601752376512145	23.658091572768004
29	23.29587099926704	26.746044376503182	25.80989695990245	24.148187664327324
30	22.193294884755133	26.895437948432882	28.95422258185266	21.957044584959327
31	21.663412808173273	27.91014867630347	26.648876914490394	23.77756160103287
32	21.182618791985423	26.73584571555374	27.247459860984407	24.83407563147643
33	20.337923104337904	25.89070173511724	32.232923967301524	21.53845119324333
34	20.399227143231787	28.61609774575971	26.609987515045823	24.374687595962673
35	21.236638073058277	26.203385955435216	29.749381916317187	22.810594055189327
36	22.366111754909365	26.602478610830303	27.379033794551898	23.65237583970843
37	22.366560047698353	27.107256291228925	27.514306143628524	23.011877517444194
38	22.237227578075792	25.77806817188442	28.48710149572889	23.497602754310893
39	23.764673183349508	26.14174569694959	26.951474547056176	23.142106572644725
40	20.96766239966647	25.236754629183412	31.526638678253544	22.268944292896574
41	21.708130013874662	26.97153564936331	26.79311511934675	24.52721921741528
42	23.159365845020698	25.28505817719669	29.501812223599476	22.05376375418313
43	22.182311711424965	25.30680037746253	29.73470032747788	22.776187583634623
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	9.0
2	15.0
3	85.0
4	155.0
5	155.0
6	198.5
7	242.0
8	261.5
9	281.0
10	460.5
11	640.0
12	640.0
13	857.0
14	1074.0
15	2771.5
16	4469.0
17	5071.0
18	5673.0
19	5673.0
20	6146.5
21	6620.0
22	8787.0
23	10954.0
24	13308.5
25	15663.0
26	15663.0
27	19181.5
28	22700.0
29	28882.0
30	35064.0
31	37469.5
32	39875.0
33	39875.0
34	43269.5
35	46664.0
36	49097.0
37	51530.0
38	56699.0
39	61868.0
40	61868.0
41	65620.0
42	69372.0
43	64669.0
44	59966.0
45	61200.5
46	62435.0
47	62435.0
48	61974.5
49	61514.0
50	63649.5
51	65785.0
52	80500.0
53	95215.0
54	95215.0
55	69866.5
56	44518.0
57	48402.0
58	52286.0
59	42281.5
60	32277.0
61	32277.0
62	24284.5
63	16292.0
64	13460.0
65	10628.0
66	8976.0
67	7324.0
68	7324.0
69	5877.5
70	4431.0
71	3762.5
72	3094.0
73	2427.0
74	1760.0
75	1760.0
76	1331.0
77	902.0
78	735.5
79	569.0
80	397.0
81	225.0
82	225.0
83	160.5
84	96.0
85	78.5
86	61.0
87	43.0
88	25.0
89	25.0
90	17.0
91	9.0
92	4.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	892274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.54420767239953
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.57525506882656	35.89160895042374
2	11.467832305587535	10.21651007544687
3	3.0925255730742287	4.132623040776744
4	1.3391768457246644	2.3861028610411354
5	0.7054759336976892	1.5712433249254913
6	0.48476135713702084	1.2955986338319416
7	0.33458540634273465	1.0432689276999467
8	0.23879902551667698	0.8509690707665194
9	0.172555536290025	0.6917714679172633
>10	1.270823947231405	11.401002353991258
>50	0.15657920916512796	4.954732025228808
>100	0.1454668122658703	12.993718722042283
>500	0.009596768864658736	2.8751436136623694
>1k	0.005556024079539268	4.077938649161158
>5k	2.5254654906996677E-4	0.7523656189128228
>10k+	7.576396472099002E-4	4.865402664171589
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGT	18063	2.0243781618650774	No Hit
ATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATG	13162	1.475107422159561	No Hit
TCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTT	12025	1.347680196890193	RNA PCR Primer, Index 27 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCC	6688	0.7495455431851651	No Hit
CTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTCTTC	4796	0.537503053994625	RNA PCR Primer, Index 27 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCGTC	3379	0.37869533349621304	RNA PCR Primer, Index 27 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2095	0.234793348231597	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1983	0.22224115013997942	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	1846	0.20688712211719718	No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC	1812	0.20307663341081328	No Hit
CACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTATGCCG	1602	0.17954126198903028	No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC	1561	0.17494626090192028	No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC	1506	0.16878223505335804	No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT	1444	0.16183369682406973	No Hit
TTATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTA	1405	0.1574628421314529	No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA	1376	0.1542127194113019	No Hit
GTTATTTCCTGTCAGTGATTTTGGTCTGAATCAACCAGTTTAT	1372	0.15376442662231557	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	1370	0.1535402802278224	No Hit
CTCTTCACCTGCTCTGTTCTACAGCACACTACCAGAAGACAGC	1163	0.13034112839777914	No Hit
ACTTTATTCCATTTGTAATTTTTGGCTACACAACACTAAAAGA	1144	0.12821173765009403	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	1089	0.12204771180153182	No Hit
ATTCAAGATCATCTTCACAACATCACCTGCTAGCCACGTGGGA	1088	0.12193563860428525	No Hit
TATACACATCTCCGAGCCCACGAGACGGACTCCTATCTCGTAT	1068	0.11969417465935353	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	1064	0.11924588187036717	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	1054	0.11812514989790132	No Hit
CTATATCACTATTCAAGATCATCTTCACAACATCACCTGCTAG	1033	0.11577161275572302	No Hit
CTTTATAAACTGGTTGATTCAGACCAAAATCACTGACAGGAAA	923	0.10344356105859859	No Hit
GGAATAACATTGCCAAACGTCACGATGAATTTGAGAGACATGC	896	0.10041758473294078	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	1.1207319724658569E-4	0.0
6	0.0	0.0	0.0	1.1207319724658569E-4	0.0
7	0.0	0.0	0.0	1.1207319724658569E-4	0.0
8	0.0	0.0	0.0	1.1207319724658569E-4	0.0
9	0.0	0.0	0.0	2.2414639449317138E-4	0.0
10	0.0	0.0	0.0	3.362195917397571E-4	0.0
11	0.0	0.0	0.0	5.603659862329285E-4	0.0
12	0.0	0.0	0.0	5.603659862329285E-4	0.0
13	0.0	0.0	0.0	5.603659862329285E-4	0.0
14	0.0	0.0	0.0	6.724391834795142E-4	0.0
15	0.0	0.0	0.0	6.724391834795142E-4	0.0
16	0.0	0.0	0.0	8.965855779726855E-4	0.0
17	0.0	0.0	0.0	0.001008658775219271	0.0
18	0.0	0.0	0.0	0.001120731972465857	0.0
19	0.0	0.0	0.0	0.001120731972465857	0.0
20	0.0	0.0	0.0	0.0012328051697124427	0.0
21	0.0	0.0	0.0	0.001456951564205614	0.0
22	0.0	0.0	0.0	0.001793171155945371	0.0
23	0.0	0.0	0.0	0.0023535371421782997	0.0
24	0.0	0.0	0.0	0.003250122720150985	0.0
25	0.0	0.0	0.0	0.003586342311890742	0.0
26	0.0	0.0	0.0	0.004034635100877084	0.0
27	0.0	0.0	0.0	0.005043293876096356	0.0
28	0.0	0.0	0.0	0.00963829496320637	0.0
29	0.0	0.0	0.0	0.019052443531919566	0.0
30	0.0	0.0	0.0	0.03328573958223595	0.0
31	0.0	0.0	0.0	0.07520111535245899	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2190	0.0	33.789955	1
TATACAC	2440	0.0	30.858606	3
TTATACA	2450	0.0	30.204082	2
CGTCTTC	655	0.0	29.656487	37
TTACCGG	25	0.005495693	29.599998	4
TTACGGG	55	6.259106E-7	26.909094	3
ACACCGT	35	8.867836E-4	26.42857	6
GGTATCA	465	0.0	25.860214	1
GCCGTCT	1995	0.0	24.852133	36
CCGTCTT	1995	0.0	24.759401	37
GATTACG	70	1.921926E-7	23.785713	1
GTATCAA	1290	0.0	23.23256	1
CCGGCGT	40	0.0019308507	23.125	23
GATACAC	60	3.7250822E-5	21.583334	3
TGCCGTC	2365	0.0	21.042284	35
GCGTCAA	45	0.0038251476	20.555555	26
ATGCCGT	2460	0.0	20.455284	34
CACGACA	55	5.141853E-4	20.18182	34
ATTACGG	75	9.261965E-6	19.733332	2
TTAACGG	95	1.675071E-7	19.473684	35
>>END_MODULE