FastQCFastQC Report
Fri 10 Feb 2017
ERR1631202.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631202.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1170493
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT211051.8030863918024287No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG161031.375745092025326No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT118411.011625016125684Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC85420.7297779653530606No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC45970.3927404948171412Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC38850.33191142535666596No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22120.1889801989418134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20840.17804463589273922No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG20270.17317489297244837No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA14660.12524637054642787No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT12550.10721977833271963No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC26250.031.7847631
TTATACA26350.031.523722
TATACAC27400.030.5182483
CGTCTTC7200.029.80555537
GGTATCA4500.026.3111111
GATTACG701.922981E-723.7857131
GTACTAG551.9026971E-523.5454541
CCGTCTT19550.023.46803337
GTATCAA14350.023.4634151
GCCGTCT19650.023.25445236
ATTCGCG400.001931208723.12530
CCGCGAA652.6829166E-622.7692327
CTTCGTA450.003825850320.55555529
ATTACCG909.478754E-820.5555555
CGCGGAA555.14317E-420.18181833
GCACCGC1101.7553248E-920.18181810
TTAACGG1101.7553248E-920.18181835
TTAGCCG851.2453474E-619.58823629
TGCGGCA951.6761805E-719.47368435
TGCCGTC23950.019.15657635
Produced by FastQC (version 0.11.5)