##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631202.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1170493
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29894070276371	31.0	31.0	34.0	28.0	34.0
2	31.297364443871086	31.0	31.0	34.0	28.0	34.0
3	30.752196723944525	31.0	31.0	34.0	26.0	34.0
4	34.94640634331004	37.0	35.0	37.0	32.0	37.0
5	35.13313791709989	37.0	35.0	37.0	32.0	37.0
6	35.32778581332823	37.0	35.0	37.0	33.0	37.0
7	35.333818314163345	37.0	35.0	37.0	33.0	37.0
8	35.35822426960264	37.0	35.0	37.0	33.0	37.0
9	37.11641248602085	39.0	37.0	39.0	33.0	39.0
10	36.91872911670553	39.0	37.0	39.0	33.0	39.0
11	36.98375556282694	39.0	37.0	39.0	33.0	39.0
12	36.91382605449157	39.0	37.0	39.0	33.0	39.0
13	37.00260146792847	39.0	37.0	39.0	33.0	39.0
14	38.09654564358779	40.0	37.0	41.0	33.0	41.0
15	38.09826628608629	40.0	37.0	41.0	33.0	41.0
16	38.0813887823336	40.0	37.0	41.0	33.0	41.0
17	38.03259310393142	40.0	37.0	41.0	33.0	41.0
18	38.08183303958246	40.0	37.0	41.0	33.0	41.0
19	38.11307372192743	40.0	37.0	41.0	33.0	41.0
20	38.10639192203627	40.0	37.0	41.0	33.0	41.0
21	38.05740657996246	40.0	37.0	41.0	33.0	41.0
22	37.9659528079194	40.0	37.0	41.0	33.0	41.0
23	37.990307502906894	40.0	37.0	41.0	33.0	41.0
24	37.939860383616136	40.0	37.0	41.0	33.0	41.0
25	37.90972180098471	40.0	37.0	41.0	33.0	41.0
26	37.791636515553705	40.0	37.0	41.0	33.0	41.0
27	37.70182820401318	40.0	37.0	41.0	32.0	41.0
28	37.699622295904376	40.0	37.0	41.0	32.0	41.0
29	37.62299048349713	39.0	37.0	41.0	32.0	41.0
30	37.56525669098406	39.0	37.0	41.0	32.0	41.0
31	37.517368322578605	39.0	37.0	41.0	32.0	41.0
32	37.42615547465897	39.0	37.0	41.0	32.0	41.0
33	37.4114368902676	39.0	37.0	41.0	32.0	41.0
34	37.34765692746561	39.0	36.0	41.0	31.0	41.0
35	37.34587648110668	39.0	36.0	41.0	31.0	41.0
36	37.3350502736881	39.0	36.0	41.0	31.0	41.0
37	37.25891483332237	39.0	36.0	41.0	31.0	41.0
38	37.23547940910368	39.0	36.0	41.0	31.0	41.0
39	37.16312955310284	39.0	36.0	40.0	31.0	41.0
40	37.077156377697264	39.0	36.0	40.0	31.0	41.0
41	37.02488353198182	39.0	36.0	40.0	31.0	41.0
42	36.964026269272864	39.0	36.0	40.0	31.0	41.0
43	36.272596247905796	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	15.0
10	16.0
11	13.0
12	15.0
13	15.0
14	11.0
15	15.0
16	18.0
17	18.0
18	23.0
19	54.0
20	113.0
21	249.0
22	477.0
23	891.0
24	1699.0
25	2936.0
26	4707.0
27	7393.0
28	10893.0
29	15393.0
30	21309.0
31	28351.0
32	37100.0
33	48318.0
34	63267.0
35	84522.0
36	116401.0
37	171740.0
38	289103.0
39	265416.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.69779058909366	22.052929833839247	14.880311116768747	25.36896846029835
2	14.419735957412815	21.43062794907787	40.28328234342282	23.866353750086503
3	14.573859049135706	28.333189519288027	33.434373379422176	23.65857805215409
4	11.904129285694149	19.481705571925676	37.95879172280398	30.655373419576197
5	12.60631204116556	37.85413496706089	36.194919576622844	13.344633415150712
6	27.82212281491645	40.13197857654852	17.718516898435105	14.327381710099932
7	25.25687893904534	32.34731006507514	23.262420193884115	19.133390801995397
8	24.74444528929263	35.15467414157966	21.958354300282018	18.14252626884569
9	24.229534051036612	14.92285729175655	19.438219621988342	41.40938903521849
10	14.573517312790424	28.395385534129638	33.958682367173495	23.07241478590645
11	31.498266115218115	23.040291569449796	25.51796550684199	19.9434768084901
12	20.506487437344777	26.26602636666772	28.93874632313051	24.288739872856993
13	28.824093779287875	20.32596521294873	26.62194477028056	24.227996237482838
14	21.759976351844905	21.71708844051182	26.0402240765216	30.482711131121672
15	24.037051054555644	28.57385734045398	22.28975312112076	25.099338483869616
16	24.91104175761837	26.830916545421456	23.743072363525457	24.514969333434717
17	21.366466950250874	30.093387999757365	25.03560465547423	23.504540394517523
18	23.860031627698756	25.843981980242514	25.442356340447997	24.853630051610732
19	23.843628283125145	26.900545325772985	25.514719011561795	23.741107379540075
20	23.88455121047285	26.005879573820607	25.21151343920895	24.898055776497596
21	24.87464683684567	26.057567196044744	25.18109890447871	23.886687062630873
22	22.156219644201204	30.17796774521505	25.72215297314892	21.943659637434827
23	24.415353188784554	25.232188488098608	25.6762748687946	24.67618345432224
24	23.237900611110017	27.552578272574035	24.99340021683171	24.216120899484235
25	23.351869682262087	27.106441473806335	26.18247183024589	23.359217013685686
26	24.434234121861472	25.89045812320108	26.388026241933954	23.287281513003492
27	22.735889919888457	29.215979933241805	25.623732905707254	22.424397241162485
28	23.80791683504301	25.18366192706834	27.126518484091743	23.881902753796904
29	22.1099998035016	26.933864619438136	25.859360115780273	25.09677546127999
30	25.086694239094125	25.498486535160826	26.569744543538494	22.84507468220656
31	24.561018305961678	25.1376983886277	26.160088099629814	24.141195205780814
32	22.460194123330936	27.941047063075132	26.31267337779893	23.286085435795005
33	23.323676433776193	25.64363904782002	28.874072719785595	22.15861179861819
34	22.161345689380457	27.065518546458627	26.03971147200368	24.733424292157235
35	21.840626129331827	25.370249971593168	29.31243501669809	23.476688882376916
36	23.326495758624784	25.667987762421475	26.791702299800168	24.21381417915357
37	22.641570688590193	26.555989655640825	27.58538496172126	23.21705469404772
38	22.52512402893482	25.283705242150102	27.941559667593058	24.249611061322025
39	23.78493506582269	25.672174032651203	26.645695446277767	23.897195455248344
40	21.81021159460159	24.677550399703374	31.086132082806134	22.426105922888905
41	22.692489404037445	25.834840533006176	25.988280152038502	25.484389910917876
42	23.063444206842757	25.303098779744943	28.798890723823213	22.834566289589088
43	23.397235182098484	24.578019689139534	29.047076744585404	22.97766838417658
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	9.5
2	14.0
3	73.0
4	132.0
5	132.0
6	162.0
7	192.0
8	217.0
9	242.0
10	345.0
11	448.0
12	448.0
13	740.5
14	1033.0
15	2306.5
16	3580.0
17	4021.5
18	4463.0
19	4463.0
20	4763.5
21	5064.0
22	6380.5
23	7697.0
24	10350.5
25	13004.0
26	13004.0
27	16717.5
28	20431.0
29	25995.0
30	31559.0
31	37408.0
32	43257.0
33	43257.0
34	50900.0
35	58543.0
36	64284.0
37	70025.0
38	75686.5
39	81348.0
40	81348.0
41	86418.5
42	91489.0
43	94266.0
44	97043.0
45	97219.0
46	97395.0
47	97395.0
48	93954.5
49	90514.0
50	86187.0
51	81860.0
52	79160.5
53	76461.0
54	76461.0
55	89035.5
56	101610.0
57	74603.0
58	47596.0
59	53108.0
60	58620.0
61	58620.0
62	48903.0
63	39186.0
64	28417.0
65	17648.0
66	14761.5
67	11875.0
68	11875.0
69	9512.5
70	7150.0
71	5932.5
72	4715.0
73	3795.0
74	2875.0
75	2875.0
76	2264.5
77	1654.0
78	1244.5
79	835.0
80	657.5
81	480.0
82	480.0
83	362.0
84	244.0
85	194.5
86	145.0
87	90.0
88	35.0
89	35.0
90	29.0
91	23.0
92	13.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1170493.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.86199074411777
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.11063247766724	35.8230910370796
2	12.455922292082533	11.425067857378785
3	3.763836629385062	5.1785112197768735
4	1.6019862341154525	2.938811113648278
5	0.8481763945178232	1.9449528977377795
6	0.5514234278050419	1.5173625685250716
7	0.36809852590842407	1.1817211831694878
8	0.26528218419302474	0.9733095260831875
9	0.20726258274777568	0.8554927186422386
>10	1.4753230449506958	13.817789926045986
>50	0.21860124787791022	6.960614641823256
>100	0.13028190394427588	10.5966999864125
>500	9.332514131634541E-4	0.2699016409423887
>1k	0.0014932022610615267	1.5867956606879352
>5k	1.8665028263269083E-4	0.7312083149159164
>10k+	5.599508478980725E-4	4.19866970713074
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGT	21105	1.8030863918024287	No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATG	16103	1.375745092025326	No Hit
TCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT	11841	1.011625016125684	Illumina PCR Primer Index 10 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCC	8542	0.7297779653530606	No Hit
CTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTC	4597	0.3927404948171412	Illumina PCR Primer Index 10 (95% over 23bp)
CATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC	3885	0.33191142535666596	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2212	0.1889801989418134	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2084	0.17804463589273922	No Hit
CACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCG	2027	0.17317489297244837	No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTA	1466	0.12524637054642787	No Hit
TATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTAT	1255	0.10721977833271963	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	8.543408632089213E-5	0.0	0.0	0.0	0.0
2	8.543408632089213E-5	0.0	0.0	8.543408632089213E-5	0.0
3	8.543408632089213E-5	0.0	0.0	8.543408632089213E-5	0.0
4	8.543408632089213E-5	0.0	0.0	8.543408632089213E-5	0.0
5	2.563022589626764E-4	0.0	0.0	8.543408632089213E-5	0.0
6	2.563022589626764E-4	0.0	0.0	8.543408632089213E-5	0.0
7	2.563022589626764E-4	0.0	0.0	8.543408632089213E-5	0.0
8	2.563022589626764E-4	0.0	0.0	8.543408632089213E-5	0.0
9	2.563022589626764E-4	0.0	0.0	8.543408632089213E-5	0.0
10	2.563022589626764E-4	0.0	0.0	1.7086817264178427E-4	0.0
11	3.4173634528356854E-4	0.0	0.0	1.7086817264178427E-4	0.0
12	3.4173634528356854E-4	0.0	0.0	1.7086817264178427E-4	0.0
13	3.4173634528356854E-4	0.0	0.0	1.7086817264178427E-4	0.0
14	3.4173634528356854E-4	0.0	0.0	1.7086817264178427E-4	0.0
15	3.4173634528356854E-4	0.0	0.0	2.563022589626764E-4	0.0
16	3.4173634528356854E-4	0.0	0.0	3.4173634528356854E-4	0.0
17	3.4173634528356854E-4	0.0	0.0	3.4173634528356854E-4	0.0
18	3.4173634528356854E-4	0.0	0.0	3.4173634528356854E-4	0.0
19	3.4173634528356854E-4	0.0	0.0	4.2717043160446066E-4	0.0
20	3.4173634528356854E-4	0.0	0.0	5.126045179253528E-4	8.543408632089213E-5
21	3.4173634528356854E-4	0.0	0.0	8.543408632089213E-4	8.543408632089213E-5
22	3.4173634528356854E-4	0.0	0.0	0.001281511294813382	8.543408632089213E-5
23	3.4173634528356854E-4	0.0	0.0	0.0013669453811342742	8.543408632089213E-5
24	3.4173634528356854E-4	0.0	0.0	0.0017941158127387348	8.543408632089213E-5
25	3.4173634528356854E-4	0.0	0.0	0.001964983985380519	8.543408632089213E-5
26	3.4173634528356854E-4	0.0	0.0	0.0021358521580223033	1.7086817264178427E-4
27	3.4173634528356854E-4	0.0	0.0	0.0035027975391565777	1.7086817264178427E-4
28	3.4173634528356854E-4	0.0	0.0	0.008970579063693675	2.563022589626764E-4
29	3.4173634528356854E-4	0.0	0.0	0.020333312544372328	2.563022589626764E-4
30	3.4173634528356854E-4	0.0	0.0	0.03605318442741648	2.563022589626764E-4
31	3.4173634528356854E-4	0.0	0.0	0.08534865223457125	2.563022589626764E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2625	0.0	31.784763	1
TTATACA	2635	0.0	31.52372	2
TATACAC	2740	0.0	30.518248	3
CGTCTTC	720	0.0	29.805555	37
GGTATCA	450	0.0	26.311111	1
GATTACG	70	1.922981E-7	23.785713	1
GTACTAG	55	1.9026971E-5	23.545454	1
CCGTCTT	1955	0.0	23.468033	37
GTATCAA	1435	0.0	23.463415	1
GCCGTCT	1965	0.0	23.254452	36
ATTCGCG	40	0.0019312087	23.125	30
CCGCGAA	65	2.6829166E-6	22.76923	27
CTTCGTA	45	0.0038258503	20.555555	29
ATTACCG	90	9.478754E-8	20.555555	5
CGCGGAA	55	5.14317E-4	20.181818	33
GCACCGC	110	1.7553248E-9	20.181818	10
TTAACGG	110	1.7553248E-9	20.181818	35
TTAGCCG	85	1.2453474E-6	19.588236	29
TGCGGCA	95	1.6761805E-7	19.473684	35
TGCCGTC	2395	0.0	19.156576	35
>>END_MODULE