FastQCFastQC Report
Fri 10 Feb 2017
ERR1631201.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631201.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences662705
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGT136242.055816690684392No Hit
ATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATG105191.5872824258154081No Hit
TCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTT84441.2741717657177778RNA PCR Primer, Index 26 (100% over 22bp)
ACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCC54480.8220852415478983No Hit
CTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTCTTC30540.46083853298224703RNA PCR Primer, Index 26 (100% over 23bp)
CATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGTC26520.40017805811032053No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21270.32095728868802864No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20700.31235617657932263No Hit
CACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCG12690.1914879169464543No Hit
TTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTA10160.15331105092009265No Hit
TATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTAT8220.12403709040975999No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT7670.11573777170837701No Hit
ACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT7110.10728755630333255RNA PCR Primer, Index 26 (95% over 21bp)
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC7020.10592948597037899No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA6910.10426962223010237No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA6660.10049720463856467No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCCGAT200.001841374137.012
TCGAACG251.2330618E-436.99999617
CTTATAC17150.033.224491
CGTCTTC4100.032.48780437
TTATACA18450.031.2845542
GTCGAAC303.5978484E-430.83333216
TATACAC19400.030.134023
CCGATGT250.005494685429.59999814
ACCGTAC250.005494685429.59999817
CCGTCTT12800.026.01562537
GGTATCA3950.025.291141
GCCGTCT13200.025.22727236
GATTACG601.3359786E-624.6666661
GTATCAA11000.023.3772741
AGTCGAA400.001930329523.12515
TTACGGG652.6800008E-622.769233
ATTACGG753.7358222E-722.1999992
TAACGGC502.7004298E-422.19999936
GGACCGT502.7004298E-422.1999996
TGCCGTC15700.021.21019235
Produced by FastQC (version 0.11.5)