FastQCFastQC Report
Fri 10 Feb 2017
ERR1631200.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631200.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences787660
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT152841.9404311504964071No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG129401.6428408196429933No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT95261.2094050732549577TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC68160.8653479927887668No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC35820.4547647462102938RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC30750.39039687174669274No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29720.37732016352233194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27740.35218241373181325No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG16500.20948124825432296No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA11710.1486682070944316No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10420.13229058223091184No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT9440.11984866566792779No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8670.11007287408272605No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT8120.10309016580758196No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT7920.10055099916207501No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18600.032.2258071
TATACAC20450.030.3056223
TTATACA20050.029.9875342
GGTATCA4750.029.61
TTACCGG502.7189526E-729.59999831
CGTCTTC5200.029.17307737
TTACGGG803.274181E-1127.7500023
TGTTACG509.086349E-625.89999816
TTTACCG601.3365134E-624.66666830
GATTACG901.4188117E-1024.6666681
CCGTCTT15100.024.2582837
GGACCGT851.9736035E-923.9411776
GCCGTCT15350.023.86319236
GACCGTT701.9213257E-723.7857137
TACGGCC701.9213257E-723.78571319
TTACGGC701.9213257E-723.78571318
CGCCGTT701.9213257E-723.78571325
GTATCAA14800.023.6251
GTTACGG753.7376776E-722.217
CCGCCGT1107.4578566E-1121.86363624
Produced by FastQC (version 0.11.5)