##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631200.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	787660
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.459105451590787	31.0	31.0	34.0	28.0	34.0
2	31.458084706599294	31.0	31.0	34.0	28.0	34.0
3	30.919862631084477	31.0	31.0	34.0	27.0	34.0
4	35.08732955843892	37.0	35.0	37.0	32.0	37.0
5	35.28638371886347	37.0	35.0	37.0	33.0	37.0
6	35.47791686768402	37.0	35.0	37.0	33.0	37.0
7	35.489162836756975	37.0	35.0	37.0	33.0	37.0
8	35.52080085315999	37.0	35.0	37.0	33.0	37.0
9	37.300335169997204	39.0	37.0	39.0	34.0	39.0
10	37.13054109641216	39.0	37.0	39.0	33.0	39.0
11	37.191521722570656	39.0	37.0	39.0	33.0	39.0
12	37.12297818855851	39.0	37.0	39.0	33.0	39.0
13	37.18288347764264	39.0	37.0	39.0	33.0	39.0
14	38.30052179874565	40.0	38.0	41.0	34.0	41.0
15	38.32282964730975	40.0	38.0	41.0	34.0	41.0
16	38.31764593860295	40.0	38.0	41.0	34.0	41.0
17	38.287092146357566	40.0	38.0	41.0	33.0	41.0
18	38.34612269253231	40.0	38.0	41.0	34.0	41.0
19	38.36332046822233	40.0	38.0	41.0	34.0	41.0
20	38.35389127288424	40.0	38.0	41.0	34.0	41.0
21	38.31315923114034	40.0	38.0	41.0	34.0	41.0
22	38.2040677449661	40.0	38.0	41.0	33.0	41.0
23	38.20475839829368	40.0	38.0	41.0	34.0	41.0
24	38.139670670086076	40.0	37.0	41.0	33.0	41.0
25	38.08373409846888	40.0	37.0	41.0	33.0	41.0
26	38.008329736180585	40.0	37.0	41.0	33.0	41.0
27	37.911022522408146	40.0	37.0	41.0	33.0	41.0
28	37.89947185333774	40.0	37.0	41.0	33.0	41.0
29	37.7906926846609	40.0	37.0	41.0	33.0	41.0
30	37.68566894345276	40.0	37.0	41.0	32.0	41.0
31	37.66940431150496	40.0	37.0	41.0	32.0	41.0
32	37.48913744509052	39.0	37.0	41.0	32.0	41.0
33	37.48165325140289	39.0	37.0	41.0	32.0	41.0
34	37.45844018992967	39.0	37.0	41.0	32.0	41.0
35	37.37444963562959	39.0	37.0	41.0	31.0	41.0
36	37.39710027169083	39.0	37.0	41.0	31.0	41.0
37	37.38593682553386	39.0	37.0	41.0	31.0	41.0
38	37.34572404336897	39.0	36.0	41.0	31.0	41.0
39	37.24318233755682	39.0	36.0	41.0	31.0	41.0
40	37.161549399487086	39.0	36.0	41.0	31.0	41.0
41	37.10692303785898	39.0	36.0	41.0	31.0	41.0
42	37.056735139527206	39.0	36.0	41.0	31.0	41.0
43	36.369323058172306	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	2.0
9	14.0
10	17.0
11	16.0
12	23.0
13	12.0
14	12.0
15	6.0
16	8.0
17	14.0
18	24.0
19	24.0
20	77.0
21	158.0
22	313.0
23	635.0
24	1179.0
25	1973.0
26	3029.0
27	4660.0
28	6775.0
29	9506.0
30	13040.0
31	17650.0
32	22843.0
33	29899.0
34	39498.0
35	53047.0
36	74451.0
37	109981.0
38	190150.0
39	208623.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.20920193992332	23.009801183251657	15.361704288652463	24.41929258817256
2	13.402864179976131	22.28220298098164	41.61376736155194	22.70116547749029
3	14.375618921869842	29.017596424853366	34.8815478759871	21.725236777289695
4	11.383845821801284	19.524541045628823	37.86100601782495	31.23060711474494
5	11.7828758601427	39.03410100804916	36.07279790772668	13.110225224081457
6	26.786430693446412	40.54414341213214	17.850722392910647	14.818703501510806
7	23.45885280450956	34.041337632988856	22.820760226493665	19.67904933600792
8	24.136556382195362	35.65028057791433	22.70598989411675	17.507173145773557
9	23.58708072010766	15.166315415280703	19.586115836782366	41.66048802782927
10	13.913490592387578	29.3979635883503	34.07421984104817	22.61432597821395
11	30.44994032958383	23.416702638194145	25.701063910824466	20.432293121397556
12	19.755224335373132	26.938399817180002	29.44861996292817	23.8577558845187
13	28.57438488688013	21.104664449127796	27.391133230073887	22.92981743391819
14	21.290531447578907	22.81073051824391	26.86539877612168	29.033339258055506
15	23.610948886575425	29.13554071553716	23.211537973237185	24.04197242465023
16	24.157758423685348	27.939466267171113	23.85483584287637	24.04793946626717
17	20.231825914734785	31.223116573140697	25.25975674783536	23.28530076428916
18	23.539725262168957	26.080923240992306	26.24508036462433	24.13427113221441
19	24.364319630297338	27.401289896655918	26.454180737881828	21.780209735164917
20	22.501713937485718	25.932635908894703	26.419648071502934	25.14600208211665
21	25.02970824975243	25.98506970012442	26.249777822918517	22.73544422720463
22	20.26381941446817	31.185536906787192	27.383134855140547	21.16750882360409
23	23.256862097859482	27.04974227458548	26.540512403829062	23.152883223725972
24	24.881420917654825	27.699134144173883	26.781352360155395	20.638092578015897
25	20.960312825330725	28.614630678211412	27.69113576924054	22.733920727217328
26	25.704618744128176	27.671203311073306	26.265647614452938	20.35853033034558
27	20.68024274433131	29.15585404870122	27.758423685346468	22.405479521621004
28	22.234593606378386	27.19777569001854	27.783180560140163	22.784450143462916
29	23.754665718711117	26.162430490313078	28.42965238808623	21.653251402889573
30	20.358657288677858	28.26727268110606	28.662874844476043	22.711195185740042
31	25.346977122108523	26.6278597364345	26.432724779727295	21.59243836172968
32	21.211055531574537	26.704288652464257	31.4177436965188	20.666912119442397
33	20.469237996089685	29.858441459512985	28.897239925856333	20.775080618540994
34	22.77150039357083	28.439936013000533	27.11715714902369	21.671406444404948
35	20.739786202168446	26.282025239316457	32.76261331031156	20.21557524820354
36	22.200314856664043	28.3721402635655	25.934032450549733	23.49351242922073
37	21.749485818754284	27.426427646446438	28.39499276337506	22.42909377142422
38	21.80395094330041	25.557854912017874	28.972145341898788	23.666048802782928
39	23.542518345479014	26.195566615036945	27.27369677271919	22.988218266764846
40	20.560521036995656	25.435213163039887	32.2767437726938	21.72752202727065
41	21.851306401239114	26.572632861894725	27.14876977376025	24.427290963105907
42	22.526978645608512	25.39217428839855	30.00279308331006	22.07805398268288
43	22.385674021786052	24.885229667623086	30.067795749435035	22.661300561155826
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	11.0
1	22.0
2	33.0
3	116.0
4	199.0
5	199.0
6	238.5
7	278.0
8	285.0
9	292.0
10	468.5
11	645.0
12	645.0
13	1041.5
14	1438.0
15	3247.0
16	5056.0
17	5709.0
18	6362.0
19	6362.0
20	6674.5
21	6987.0
22	8471.5
23	9956.0
24	12569.5
25	15183.0
26	15183.0
27	18616.0
28	22049.0
29	26346.0
30	30643.0
31	33712.5
32	36782.0
33	36782.0
34	39664.5
35	42547.0
36	44874.0
37	47201.0
38	50082.0
39	52963.0
40	52963.0
41	54524.5
42	56086.0
43	54200.0
44	52314.0
45	53062.5
46	53811.0
47	53811.0
48	54232.5
49	54654.0
50	67373.5
51	80093.0
52	65940.5
53	51788.0
54	51788.0
55	54742.5
56	57697.0
57	48762.5
58	39828.0
59	30444.0
60	21060.0
61	21060.0
62	18290.0
63	15520.0
64	12729.0
65	9938.0
66	8298.0
67	6658.0
68	6658.0
69	5324.5
70	3991.0
71	3329.0
72	2667.0
73	2097.0
74	1527.0
75	1527.0
76	1133.5
77	740.0
78	577.5
79	415.0
80	284.0
81	153.0
82	153.0
83	104.0
84	55.0
85	40.5
86	26.0
87	18.5
88	11.0
89	11.0
90	6.5
91	2.0
92	1.5
93	1.0
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	787660.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.83893943739757
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.19086359061133	42.088482605437086
2	11.181945699925505	11.59320411861413
3	3.091899869222731	4.808424302014039
4	1.287688053926818	2.6700953216709062
5	0.6769076637982158	1.7545087694172992
6	0.4246357578723042	1.3207600401177477
7	0.313884120279166	1.1389993931058748
8	0.2329189062259655	0.9659415258938167
9	0.16616240004694988	0.7752314333525816
>10	1.2080283707712811	12.532357715522863
>50	0.14645195747036724	5.33985434166434
>100	0.07396046783354707	6.57003314702992
>500	0.0019592176908662094	0.7191984266371856
>1k	0.0017143154795079335	2.0650452970309727
>5k	4.898044227165523E-4	2.0746938549170144
>10k+	4.898044227165523E-4	3.5831697075742146
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGT	15284	1.9404311504964071	No Hit
ATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATG	12940	1.6428408196429933	No Hit
TCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTT	9526	1.2094050732549577	TruSeq Adapter, Index 12 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCC	6816	0.8653479927887668	No Hit
CTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTCTTC	3582	0.4547647462102938	RNA PCR Primer, Index 12 (95% over 23bp)
CATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGTC	3075	0.39039687174669274	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2972	0.37732016352233194	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2774	0.35218241373181325	No Hit
CACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCG	1650	0.20948124825432296	No Hit
TTATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTA	1171	0.1486682070944316	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1042	0.13229058223091184	No Hit
TATACACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTAT	944	0.11984866566792779	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	867	0.11007287408272605	No Hit
GTTTTATTGTCTCTGCCTGGACTTCTAACATAGCATATGAGGT	812	0.10309016580758196	No Hit
ACATCTCCGAGCCCACGAGACGCGTAGTAATCTCGTATGCCGT	792	0.10055099916207501	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	2.5391666455069445E-4	0.0
6	0.0	0.0	0.0	2.5391666455069445E-4	0.0
7	0.0	0.0	0.0	3.808749968260417E-4	0.0
8	0.0	0.0	0.0	3.808749968260417E-4	0.0
9	0.0	0.0	0.0	3.808749968260417E-4	0.0
10	0.0	0.0	0.0	3.808749968260417E-4	0.0
11	0.0	0.0	0.0	5.078333291013889E-4	0.0
12	0.0	0.0	0.0	5.078333291013889E-4	0.0
13	0.0	0.0	0.0	5.078333291013889E-4	0.0
14	0.0	0.0	0.0	5.078333291013889E-4	0.0
15	0.0	0.0	0.0	6.347916613767361E-4	0.0
16	0.0	0.0	0.0	6.347916613767361E-4	0.0
17	0.0	0.0	0.0	6.347916613767361E-4	0.0
18	0.0	0.0	0.0	6.347916613767361E-4	0.0
19	0.0	0.0	0.0	6.347916613767361E-4	0.0
20	0.0	0.0	0.0	6.347916613767361E-4	0.0
21	0.0	0.0	0.0	8.887083259274307E-4	0.0
22	0.0	0.0	0.0	0.0010156666582027778	0.0
23	0.0	0.0	0.0	0.001650458319579514	0.0
24	0.0	0.0	0.0	0.0019043749841302084	0.0
25	0.0	0.0	0.0	0.0019043749841302084	0.0
26	0.0	0.0	0.0	0.0019043749841302084	0.0
27	0.0	0.0	0.0	0.0024122083132315972	0.0
28	0.0	0.0	0.0	0.008887083259274306	0.0
29	0.0	0.0	0.0	0.020313333164055557	0.0
30	0.0	0.0	0.0	0.03770662468577813	0.0
31	0.0	0.0	0.0	0.08226899931442501	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1860	0.0	32.225807	1
TATACAC	2045	0.0	30.305622	3
TTATACA	2005	0.0	29.987534	2
GGTATCA	475	0.0	29.6	1
TTACCGG	50	2.7189526E-7	29.599998	31
CGTCTTC	520	0.0	29.173077	37
TTACGGG	80	3.274181E-11	27.750002	3
TGTTACG	50	9.086349E-6	25.899998	16
TTTACCG	60	1.3365134E-6	24.666668	30
GATTACG	90	1.4188117E-10	24.666668	1
CCGTCTT	1510	0.0	24.25828	37
GGACCGT	85	1.9736035E-9	23.941177	6
GCCGTCT	1535	0.0	23.863192	36
GACCGTT	70	1.9213257E-7	23.785713	7
TACGGCC	70	1.9213257E-7	23.785713	19
TTACGGC	70	1.9213257E-7	23.785713	18
CGCCGTT	70	1.9213257E-7	23.785713	25
GTATCAA	1480	0.0	23.625	1
GTTACGG	75	3.7376776E-7	22.2	17
CCGCCGT	110	7.4578566E-11	21.863636	24
>>END_MODULE