FastQCFastQC Report
Fri 10 Feb 2017
ERR1631199.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631199.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810630
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT128531.5855569125248263No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG99011.2213957045754538No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT93611.1547808494627636RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC48220.5948459839877627No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA45680.5635123299162379No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC37330.46050602617717085RNA PCR Primer, Index 17 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT26810.33073041955022636No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25960.32024474791211777No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC25700.3170373659992845No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA13050.1609858998556678No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC12760.15740843541443075No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG11130.13730061803782243No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA10570.13039241084095088No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10090.12447109038648953No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG9870.12175715184486141No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG9500.11719280066121412No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA9430.11632927476160518No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT8700.10732393323711188No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC15300.031.8006531
GATTACG801.8189894E-1230.06251
CGTCTTC5500.027.91818237
TATACAC17750.027.7239463
TTATACA18050.026.8531862
GCTTTAT8300.024.7409611
GCCGTCT15200.024.34210636
CCGTCTT15300.024.18300637
GGTATCA4850.024.0309281
GTATCAA14350.023.5923351
TTATTGA8850.022.5762714
ATTGAGC8600.022.3720936
TATTGAG8900.022.0337095
CTTTATT10800.020.8981482
GCTACCG450.003824850320.5555557
TACCGTT450.003824850320.5555559
TGCCGTC18000.020.55555535
TTGAGCC9350.020.379687
TGAGCCT9350.020.379688
AGCCTGT9650.020.32124310
Produced by FastQC (version 0.11.5)