##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631199.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	810630
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.494105818930954	31.0	31.0	34.0	28.0	34.0
2	31.49687156902656	31.0	31.0	34.0	28.0	34.0
3	30.991161195613287	31.0	31.0	34.0	27.0	34.0
4	35.13446701947868	37.0	35.0	37.0	32.0	37.0
5	35.316057880907444	37.0	35.0	37.0	33.0	37.0
6	35.50377360818129	37.0	35.0	37.0	33.0	37.0
7	35.51215474384121	37.0	35.0	37.0	33.0	37.0
8	35.53233040968136	37.0	35.0	37.0	33.0	37.0
9	37.300595832870734	39.0	37.0	39.0	34.0	39.0
10	37.15470436574023	39.0	37.0	39.0	33.0	39.0
11	37.2035318209294	39.0	37.0	39.0	33.0	39.0
12	37.14287282730716	39.0	37.0	39.0	33.0	39.0
13	37.21757645288233	39.0	37.0	39.0	34.0	39.0
14	38.356333962473634	40.0	38.0	41.0	34.0	41.0
15	38.37704131354626	40.0	38.0	41.0	34.0	41.0
16	38.36711076570075	40.0	38.0	41.0	34.0	41.0
17	38.342444765182634	40.0	38.0	41.0	34.0	41.0
18	38.38276032221852	40.0	38.0	41.0	34.0	41.0
19	38.399694065109856	40.0	38.0	41.0	34.0	41.0
20	38.41150093137436	40.0	38.0	41.0	34.0	41.0
21	38.354576070463715	40.0	38.0	41.0	34.0	41.0
22	38.29724041794653	40.0	38.0	41.0	34.0	41.0
23	38.30564375855816	40.0	38.0	41.0	34.0	41.0
24	38.26163724510566	40.0	38.0	41.0	34.0	41.0
25	38.22710977881401	40.0	38.0	41.0	34.0	41.0
26	38.11276784722993	40.0	37.0	41.0	33.0	41.0
27	38.04778628967593	40.0	37.0	41.0	33.0	41.0
28	38.02296855532117	40.0	37.0	41.0	33.0	41.0
29	37.962614262980644	40.0	37.0	41.0	33.0	41.0
30	37.89642253555876	40.0	37.0	41.0	33.0	41.0
31	37.858387920506274	40.0	37.0	41.0	33.0	41.0
32	37.779567743606826	40.0	37.0	41.0	33.0	41.0
33	37.74887186509258	40.0	37.0	41.0	33.0	41.0
34	37.70988737155052	40.0	37.0	41.0	33.0	41.0
35	37.6534966630892	40.0	37.0	41.0	33.0	41.0
36	37.634151215721104	40.0	37.0	41.0	33.0	41.0
37	37.58723215277007	40.0	37.0	41.0	32.0	41.0
38	37.55049406017542	40.0	37.0	41.0	32.0	41.0
39	37.46854668591096	40.0	37.0	41.0	32.0	41.0
40	37.380658253457185	39.0	37.0	41.0	32.0	41.0
41	37.353898819436736	39.0	37.0	41.0	32.0	41.0
42	37.310480737204394	39.0	36.0	41.0	31.0	41.0
43	36.701507469499035	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	8.0
10	8.0
11	9.0
12	9.0
13	6.0
14	3.0
15	5.0
16	11.0
17	7.0
18	17.0
19	20.0
20	58.0
21	106.0
22	252.0
23	521.0
24	987.0
25	1641.0
26	2726.0
27	4227.0
28	6520.0
29	9225.0
30	13027.0
31	17188.0
32	22556.0
33	29723.0
34	39039.0
35	52766.0
36	73259.0
37	109538.0
38	193049.0
39	234118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.90504915929586	22.114528206456708	15.108742582929327	23.871680051318112
2	13.637664532524084	22.06481378680779	40.54735205950927	23.75016962115885
3	14.809592539136226	28.26492974600002	34.5849524443951	22.340525270468646
4	11.519928944154547	18.670046753759422	39.16361348580734	30.646410816278696
5	12.479182857777284	37.99958057313448	36.330755091718785	13.190481477369453
6	27.90705994103352	40.56178527811702	17.32121929857025	14.209935482279215
7	24.442594031802425	33.02567139138694	23.498760223529846	19.032974353280782
8	25.05014618259872	34.50094371044743	22.53099441175382	17.91791569520003
9	24.109519756238974	15.25578870754845	19.980879069365802	40.65381246684677
10	14.222641649087747	29.450427445320305	33.81160332087389	22.515327584718058
11	31.388796368256788	23.578944771350677	25.426890196513824	19.60536866387871
12	21.118266039993586	26.432157704501435	29.21061396691462	23.238962288590358
13	28.695582448219287	20.913857123471868	26.722549128455647	23.6680112998532
14	21.234965397283595	22.20248448737402	27.62036934236335	28.942180772979043
15	23.716862193602505	29.70455078149094	23.156803967284702	23.42178305762185
16	24.161578031901115	27.639860355525947	24.443951001073238	23.7546106114997
17	21.554716701824507	30.082651764676854	25.320799871704725	23.041831661793914
18	23.19788312793753	26.50457051922579	27.037242638441704	23.26030371439498
19	24.397073880808755	28.03301136153362	26.4961819819153	21.073732775742325
20	24.966384170336653	25.807335035712963	25.837064998828076	23.38921579512231
21	24.865475000925205	25.989045557159248	26.05035589603148	23.095123545884068
22	21.38916645078519	30.290391423954212	26.619172742188173	21.701269383072425
23	23.684048209417366	26.887235853595353	26.17667739905999	23.25203853792729
24	23.761642179539372	28.291699048887903	26.332358782675204	21.61429998889752
25	22.35125766379236	30.155434661929608	25.867288405314387	21.626019268963645
26	24.40546241811924	29.021625155743063	25.916509381592096	20.656403044545602
27	22.949557751378556	28.629337675635004	26.656180995028556	21.764923577957884
28	22.910699085896155	26.91893959019528	27.404734589146713	22.765626734761852
29	22.491025498686206	28.44750379334592	26.39107854384861	22.670392164119267
30	23.922381357709437	27.868324636393915	26.842825950186892	21.366468055709756
31	24.645029174839323	26.655934273342957	26.24723980114232	22.451796750675403
32	21.86496922146972	28.319825321046594	27.198228538297375	22.616976919186314
33	20.532548758373117	29.811504632199647	28.389647558072117	21.26629905135512
34	23.86847266940528	27.774817117550548	26.8440595586149	21.512650654429272
35	21.416675918729876	27.672304257182684	29.40453721179823	21.506482612289208
36	22.309685059768327	28.201522272800165	26.552681247918287	22.93611141951322
37	22.044829330273984	26.870458778974378	28.53977770376127	22.544934186990364
38	22.386785586519128	25.46722919210984	28.646361471941578	23.499623749429457
39	24.037847106571434	26.027410779270443	26.983087228451947	22.95165488570618
40	21.342289330520707	25.341524493295335	31.055968814378943	22.260217361805015
41	21.61874097923837	26.339883794086084	27.111629226650873	24.92974600002467
42	22.448466007919766	25.426273392299812	29.588344867572136	22.536915732208282
43	22.73416971984753	25.67052786104635	29.334098170558704	22.261204248547426
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	18.0
1	34.0
2	50.0
3	168.5
4	287.0
5	287.0
6	366.5
7	446.0
8	411.5
9	377.0
10	540.5
11	704.0
12	704.0
13	1081.0
14	1458.0
15	3106.5
16	4755.0
17	5460.5
18	6166.0
19	6166.0
20	6648.5
21	7131.0
22	8993.0
23	10855.0
24	13689.5
25	16524.0
26	16524.0
27	20179.5
28	23835.0
29	27691.5
30	31548.0
31	34830.5
32	38113.0
33	38113.0
34	41375.5
35	44638.0
36	46664.5
37	48691.0
38	50465.0
39	52239.0
40	52239.0
41	52774.5
42	53310.0
43	52761.5
44	52213.0
45	52348.0
46	52483.0
47	52483.0
48	53787.0
49	55091.0
50	63900.5
51	72710.0
52	59224.0
53	45738.0
54	45738.0
55	50938.0
56	56138.0
57	48007.5
58	39877.0
59	34113.0
60	28349.0
61	28349.0
62	25702.5
63	23056.0
64	20270.0
65	17484.0
66	14346.0
67	11208.0
68	11208.0
69	9345.0
70	7482.0
71	6321.0
72	5160.0
73	3291.0
74	1422.0
75	1422.0
76	986.5
77	551.0
78	401.5
79	252.0
80	194.5
81	137.0
82	137.0
83	102.5
84	68.0
85	57.0
86	46.0
87	31.0
88	16.0
89	16.0
90	9.0
91	2.0
92	2.0
93	2.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	810630.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.30151074716312
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.4134158328615	46.963013279714374
2	10.496193396797796	11.819030906682274
3	2.5563304848258555	4.317758047941708
4	1.0247062685894044	2.3077004397468706
5	0.5441846092191671	1.531920781219685
6	0.33750142321981347	1.1401104003955906
7	0.22555371224707832	0.8889310327898992
8	0.16468309732667133	0.7417525739210956
9	0.127094103777002	0.6440031044731749
>10	0.9425147827956835	10.507286210919728
>50	0.09946685065969063	3.9200857319651448
>100	0.0620018873841153	6.624502126068897
>500	0.0032863191187494424	1.3353851598572348
>1k	0.0024099673537495915	3.2971407096789105
>5k	4.3817588249992574E-4	2.3759642480297485
>10k+	2.1908794124996287E-4	1.5854152465956992
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGT	12853	1.5855569125248263	No Hit
ATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATG	9901	1.2213957045754538	No Hit
TCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTT	9361	1.1547808494627636	RNA PCR Primer, Index 17 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCC	4822	0.5948459839877627	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4568	0.5635123299162379	No Hit
CTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTCTTC	3733	0.46050602617717085	RNA PCR Primer, Index 17 (95% over 22bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2681	0.33073041955022636	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2596	0.32024474791211777	No Hit
CATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCGTC	2570	0.3170373659992845	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1305	0.1609858998556678	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1276	0.15740843541443075	No Hit
CACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTATGCCG	1113	0.13730061803782243	No Hit
TTATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTA	1057	0.13039241084095088	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1009	0.12447109038648953	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	987	0.12175715184486141	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	950	0.11719280066121412	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	943	0.11632927476160518	No Hit
TATACACATCTCCGAGCCCACGAGACAGGCAGAAATCTCGTAT	870	0.10732393323711188	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.4672168560255605E-4	0.0
11	0.0	0.0	0.0	2.4672168560255605E-4	0.0
12	0.0	0.0	0.0	3.700825284038341E-4	0.0
13	0.0	0.0	0.0	7.401650568076681E-4	0.0
14	0.0	0.0	0.0	8.635258996089461E-4	0.0
15	0.0	0.0	0.0	9.868867424102242E-4	0.0
16	0.0	0.0	0.0	9.868867424102242E-4	0.0
17	0.0	0.0	0.0	0.0012336084280127801	0.0
18	0.0	0.0	0.0	0.0012336084280127801	0.0
19	0.0	0.0	0.0	0.0013569692708140582	0.0
20	0.0	0.0	0.0	0.0013569692708140582	0.0
21	0.0	0.0	0.0	0.0018504126420191703	0.0
22	0.0	0.0	0.0	0.0024672168560255603	0.0
23	0.0	0.0	0.0	0.0034541035984357845	0.0
24	0.0	0.0	0.0	0.004070907812442175	0.0
25	0.0	0.0	0.0	0.004564351183647287	0.0
26	0.0	0.0	0.0	0.004687712026448565	0.0
27	0.0	0.0	0.0	0.005674598768858789	0.0
28	0.0	0.0	0.0	0.0112258366949163	0.0
29	0.0	0.0	0.0	0.022821755918236434	0.0
30	0.0	0.0	0.0	0.036514809469178296	0.0
31	0.0	0.0	0.0	0.08289848636245883	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1530	0.0	31.800653	1
GATTACG	80	1.8189894E-12	30.0625	1
CGTCTTC	550	0.0	27.918182	37
TATACAC	1775	0.0	27.723946	3
TTATACA	1805	0.0	26.853186	2
GCTTTAT	830	0.0	24.740961	1
GCCGTCT	1520	0.0	24.342106	36
CCGTCTT	1530	0.0	24.183006	37
GGTATCA	485	0.0	24.030928	1
GTATCAA	1435	0.0	23.592335	1
TTATTGA	885	0.0	22.576271	4
ATTGAGC	860	0.0	22.372093	6
TATTGAG	890	0.0	22.033709	5
CTTTATT	1080	0.0	20.898148	2
GCTACCG	45	0.0038248503	20.555555	7
TACCGTT	45	0.0038248503	20.555555	9
TGCCGTC	1800	0.0	20.555555	35
TTGAGCC	935	0.0	20.37968	7
TGAGCCT	935	0.0	20.37968	8
AGCCTGT	965	0.0	20.321243	10
>>END_MODULE