FastQCFastQC Report
Fri 10 Feb 2017
ERR1631198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences827801
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG170712.0622106037562165No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT143361.731817187947345No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT129261.5614863958850014TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC86781.0483195840546218No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC52910.6391632771644393RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC37960.45856431678628073TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21550.2603282673009576No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG21080.25465057423221277No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT20690.2499392970049565No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA14030.16948517820104106No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT12500.151002475232574No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT10270.12406363365108282No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8790.10618494058354604No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8590.10376890097982486No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC19900.032.6306531
CGTCTTC7250.030.36551937
TTATACA21800.029.871562
TATACAC22050.029.868483
GGTATCA4350.027.6436791
TATATCG451.322775E-424.6666685
CCGTCTT20900.023.81100537
GCCGTCT20900.023.81100536
CGGTGTA400.00193073323.12500234
TCGCCGA652.6813395E-622.769231
GTATCAA13350.022.5880151
CGAACTA753.7381506E-722.229
GATTACG603.7246646E-521.5833341
TAAGCCG450.00382491720.55555735
ACGGTTT450.00382491720.55555727
AACACCG450.00382491720.5555575
GCCGCGT555.141423E-420.18181836
ATCGGAG555.141423E-420.18181818
TGCCGTC24850.020.02615735
ATAATCG656.899148E-519.92307715
Produced by FastQC (version 0.11.5)