##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631198.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	827801
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.681323168249374	31.0	30.0	33.0	27.0	34.0
2	30.671661425874092	31.0	30.0	34.0	27.0	34.0
3	30.090270487713834	31.0	30.0	34.0	25.0	34.0
4	34.35283600768784	35.0	35.0	37.0	30.0	37.0
5	34.36425058679562	35.0	35.0	37.0	30.0	37.0
6	34.614338470236206	35.0	35.0	37.0	31.0	37.0
7	34.63820894152097	35.0	35.0	37.0	32.0	37.0
8	34.68274863161557	35.0	35.0	37.0	32.0	37.0
9	36.369709628280226	38.0	35.0	39.0	32.0	39.0
10	36.07591558840833	37.0	35.0	39.0	32.0	39.0
11	36.193568260970935	37.0	35.0	39.0	32.0	39.0
12	36.07896704642783	37.0	35.0	39.0	32.0	39.0
13	36.22544669552223	37.0	35.0	39.0	32.0	39.0
14	37.12256085701757	38.0	36.0	40.0	32.0	41.0
15	37.12086842127516	38.0	36.0	40.0	32.0	41.0
16	37.12353089691846	38.0	36.0	40.0	32.0	41.0
17	37.024313814552045	38.0	36.0	40.0	31.0	41.0
18	37.128070635333856	38.0	36.0	40.0	32.0	41.0
19	37.12927503107631	39.0	36.0	40.0	31.0	41.0
20	37.114597590483704	39.0	36.0	40.0	31.0	41.0
21	37.027558555739844	39.0	36.0	40.0	31.0	41.0
22	36.86466312555795	39.0	36.0	40.0	31.0	41.0
23	36.93766255416459	39.0	36.0	40.0	31.0	41.0
24	36.83009926298712	38.0	36.0	40.0	31.0	41.0
25	36.807913979325946	38.0	36.0	40.0	31.0	41.0
26	36.65782476706358	38.0	35.0	40.0	30.0	41.0
27	36.48245774044728	38.0	35.0	40.0	30.0	41.0
28	36.49158433005034	38.0	35.0	40.0	30.0	41.0
29	36.38663156966469	38.0	35.0	40.0	30.0	41.0
30	36.320891132047436	38.0	35.0	40.0	30.0	41.0
31	36.308870126999125	38.0	35.0	40.0	30.0	41.0
32	36.162026863944355	38.0	35.0	40.0	30.0	41.0
33	36.17398988404218	38.0	35.0	40.0	30.0	41.0
34	36.10980054385052	38.0	35.0	40.0	30.0	41.0
35	36.10767442899924	38.0	35.0	40.0	30.0	41.0
36	36.116884371968624	38.0	35.0	40.0	30.0	41.0
37	36.03873877900607	38.0	35.0	40.0	29.0	41.0
38	35.980257332378194	38.0	35.0	40.0	29.0	41.0
39	35.898346341693234	38.0	34.0	40.0	29.0	41.0
40	35.770007525963365	38.0	34.0	40.0	28.0	41.0
41	35.74259151656014	38.0	34.0	40.0	28.0	41.0
42	35.66475880072626	38.0	34.0	40.0	28.0	41.0
43	34.859386495063426	38.0	33.0	40.0	26.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	8.0
10	10.0
11	16.0
12	15.0
13	17.0
14	16.0
15	18.0
16	9.0
17	27.0
18	46.0
19	83.0
20	168.0
21	366.0
22	742.0
23	1330.0
24	2257.0
25	3754.0
26	5711.0
27	8568.0
28	12337.0
29	16993.0
30	22768.0
31	29463.0
32	37807.0
33	48463.0
34	63332.0
35	83078.0
36	111856.0
37	149266.0
38	169695.0
39	59578.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.743734303292705	23.66039664122174	15.368186315310082	24.227682740175478
2	14.642045612411678	22.493932720545153	39.8961827782281	22.967838888815066
3	15.815032840018315	29.021830125839422	33.53740814519431	21.625728888947947
4	12.041420582966197	19.652307740628487	36.88205257060574	31.424219105799583
5	12.370485176993022	38.73600056052119	35.31851254105757	13.575001721428217
6	27.411539729959255	40.2809370851207	17.277220008190376	15.030303176729673
7	24.099874245138626	33.92204165010673	22.325413958185603	19.652670146569044
8	24.82480692823517	34.90899382822683	22.51918033440404	17.747018909133956
9	23.590693898654386	14.566544374795393	19.293646661456073	42.54911506509414
10	14.108825671870411	28.890035165456435	33.70930936299908	23.291829799674076
11	31.482808066189822	22.798836918534768	24.800042522297026	20.918312492978387
12	19.992606918812612	26.90707066070227	28.843163997144238	24.257158423340876
13	29.807405402989367	20.534524601927277	26.119441749889166	23.538628245194197
14	21.449841205797046	22.848969740311983	26.14323973998582	29.55794931390515
15	24.136356443154817	29.316466155513222	22.03464359187776	24.512533809454204
16	24.568585928260536	27.525455997274705	23.260904492746445	24.64505358171831
17	20.556510562321137	30.903562571197668	24.60857138370212	23.931355482779075
18	24.244232611460966	25.459742136093094	25.200501086613812	25.095524165832128
19	25.668850363795165	27.534395343808477	25.076075047022172	21.720679245374193
20	23.626934492710205	26.888950363674365	25.007217918316115	24.476897225299318
21	25.51229099747403	27.146862591371594	24.92748861139332	22.413357799761055
22	22.896082512584545	30.211608828691922	25.25788202720219	21.634426631521343
23	23.443073878867022	25.520867938067244	26.098422205336792	24.93763597772895
24	24.354283215410465	28.389673363525773	26.437513363719056	20.8185300573447
25	22.55348809677688	29.10602910602911	25.205816373741996	23.134666423452014
26	25.428816829165463	27.144446551767874	25.33302085887792	22.093715760188744
27	24.15375192830161	29.11206920503841	25.759935056855454	20.97424380980453
28	20.81297316625614	27.329031977492175	27.4033251953066	24.454669660945083
29	25.441863443025557	25.723936066760007	26.798107274574445	22.03609321563999
30	22.85150658189589	26.54333589836205	27.122702195334387	23.482455324407677
31	22.33833977006551	26.807409027048774	27.321904660661193	23.53234654222452
32	23.910577542187074	28.246885422945855	26.777208532002255	21.06532850286482
33	20.512055433612666	25.681896977655256	28.583077333803658	25.222970254928416
34	21.15194352265822	28.56544024469649	28.225986680373666	22.05662955227162
35	21.20050591869302	27.379043997289205	30.82250444249282	20.597945641524955
36	23.053970700687724	25.527149641036917	25.063028433162078	26.35585122511328
37	22.133822017610512	26.474357967675804	28.28095158135832	23.110868433355357
38	21.91843208693877	25.049015403460494	28.29291097739674	24.739641532203997
39	24.534519769848067	25.602771680633392	26.677667700328943	23.1850408491896
40	21.020631770195976	24.614369878751052	32.37879635322982	21.986201997823148
41	22.592627938357165	26.285544472644993	26.163776076617445	24.95805151238039
42	22.381466076991934	24.92471016584904	30.356087996994447	22.33773576016458
43	23.2743135125471	24.45503206688564	29.413953353523368	22.85670106704389
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	10.5
2	17.0
3	61.0
4	105.0
5	105.0
6	160.0
7	215.0
8	234.5
9	254.0
10	371.0
11	488.0
12	488.0
13	748.0
14	1008.0
15	2283.0
16	3558.0
17	4042.0
18	4526.0
19	4526.0
20	4932.5
21	5339.0
22	6605.0
23	7871.0
24	10265.5
25	12660.0
26	12660.0
27	16000.0
28	19340.0
29	23178.0
30	27016.0
31	30886.0
32	34756.0
33	34756.0
34	39095.5
35	43435.0
36	46222.0
37	49009.0
38	51544.5
39	54080.0
40	54080.0
41	56376.5
42	58673.0
43	59140.0
44	59607.0
45	59752.0
46	59897.0
47	59897.0
48	58664.0
49	57431.0
50	57216.5
51	57002.0
52	69573.5
53	82145.0
54	82145.0
55	63422.5
56	44700.0
57	50689.0
58	56678.0
59	46983.5
60	37289.0
61	37289.0
62	28078.0
63	18867.0
64	15718.5
65	12570.0
66	10277.5
67	7985.0
68	7985.0
69	6461.5
70	4938.0
71	3942.5
72	2947.0
73	2328.0
74	1709.0
75	1709.0
76	1273.0
77	837.0
78	629.0
79	421.0
80	324.5
81	228.0
82	228.0
83	165.5
84	103.0
85	81.5
86	60.0
87	42.0
88	24.0
89	24.0
90	15.5
91	7.0
92	4.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	827801.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.29389047048675
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.69368648669135	45.17161752725394
2	10.90026639332862	12.054362721036807
3	2.982116020050362	4.946783897488457
4	1.2431131223146328	2.749462433107604
5	0.7067021567199193	1.9538155824463987
6	0.4466015508219654	1.4816602341059566
7	0.3066814335203652	1.1870326716084856
8	0.23991130928860158	1.0612503726748004
9	0.17649222572645465	0.8783047618389908
>10	1.1586291882728097	12.298038783673922
>50	0.10597755456186633	3.98532953246118
>100	0.03634080493876192	3.212191384250233
>500	8.704384410840225E-4	0.3435273598338159
>1k	0.0015232672718970394	1.6614451084361925
>5k	4.3521922054201123E-4	1.6808174043847897
>10k+	6.528288308130168E-4	5.334360225398445
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
ATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATG	17071	2.0622106037562165	No Hit
CTTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGT	14336	1.731817187947345	No Hit
TCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTT	12926	1.5614863958850014	TruSeq Adapter, Index 11 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCC	8678	1.0483195840546218	No Hit
CTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTCTTC	5291	0.6391632771644393	RNA PCR Primer, Index 11 (95% over 24bp)
CATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGTC	3796	0.45856431678628073	TruSeq Adapter, Index 11 (95% over 21bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2155	0.2603282673009576	No Hit
CACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCG	2108	0.25465057423221277	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2069	0.2499392970049565	No Hit
TTATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTA	1403	0.16948517820104106	No Hit
TATACACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTAT	1250	0.151002475232574	No Hit
ACATCTCCGAGCCCACGAGACGGAGCTACATCTCGTATGCCGT	1027	0.12406363365108282	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	879	0.10618494058354604	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	859	0.10376890097982486	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	1.2080198018605921E-4	0.0
16	0.0	0.0	0.0	1.2080198018605921E-4	0.0
17	0.0	0.0	0.0	2.4160396037211843E-4	0.0
18	0.0	0.0	0.0	3.624059405581776E-4	0.0
19	0.0	0.0	0.0	6.04009900930296E-4	0.0
20	0.0	0.0	0.0	6.04009900930296E-4	0.0
21	0.0	0.0	0.0	7.248118811163552E-4	0.0
22	0.0	0.0	0.0	7.248118811163552E-4	0.0
23	0.0	0.0	0.0	0.0010872178216745328	0.0
24	0.0	0.0	0.0	0.001208019801860592	0.0
25	0.0	0.0	0.0	0.0014496237622327105	0.0
26	0.0	0.0	0.0	0.0018120297027908881	0.0
27	0.0	0.0	0.0	0.002295237623535125	0.0
28	0.0	0.0	0.0	0.006281702969675079	0.0
29	0.0	0.0	0.0	0.015100247523257402	0.0
30	0.0	0.0	0.0	0.027180445541863323	0.0
31	0.0	0.0	0.0	0.0636626435580532	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1990	0.0	32.630653	1
CGTCTTC	725	0.0	30.365519	37
TTATACA	2180	0.0	29.87156	2
TATACAC	2205	0.0	29.86848	3
GGTATCA	435	0.0	27.643679	1
TATATCG	45	1.322775E-4	24.666668	5
CCGTCTT	2090	0.0	23.811005	37
GCCGTCT	2090	0.0	23.811005	36
CGGTGTA	40	0.001930733	23.125002	34
TCGCCGA	65	2.6813395E-6	22.76923	1
GTATCAA	1335	0.0	22.588015	1
CGAACTA	75	3.7381506E-7	22.2	29
GATTACG	60	3.7246646E-5	21.583334	1
TAAGCCG	45	0.003824917	20.555557	35
ACGGTTT	45	0.003824917	20.555557	27
AACACCG	45	0.003824917	20.555557	5
GCCGCGT	55	5.141423E-4	20.181818	36
ATCGGAG	55	5.141423E-4	20.181818	18
TGCCGTC	2485	0.0	20.026157	35
ATAATCG	65	6.899148E-5	19.923077	15
>>END_MODULE