FastQCFastQC Report
Fri 10 Feb 2017
ERR1631197.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631197.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences367597
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT175074.7625524691441985No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG138943.7796826415884786No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT114053.1025824476260686TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC70201.9097000247553706No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC46911.2761257572831117RNA PCR Primer, Index 11 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35850.9752527904199436No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC34640.9423363085117671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT32370.8805839003038654No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA19220.5228551919629376No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG15290.4159446350215045No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA14350.3903731532085409No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13100.35636852313811046No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT11890.3234520412299339No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11240.30576963359331005No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10530.2864550037133056No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT10000.27203704056344313No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8770.2385764845741396No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT8050.2189898176535717No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7990.21735759541019103No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT7920.21545333612624692No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7630.2075642619499071No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7060.19205815063779083No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6920.18824963206990264No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5470.1488042611882034No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5220.1420033351741173No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT5090.13846685364679256No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4960.1349303721194678No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4920.133842223957214No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4900.13329814987608712No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC4860.13221000171383335No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4810.13084981651101613No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4590.12486500161862038No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA4420.12024037192904186No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4400.11969629784791497No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG4350.11833611264509775No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4260.11588777928002678No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4040.10990296438763102No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG3990.1085427791848138No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3990.1085427791848138No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3980.10827074214425035No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT3940.1071825939819966No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC3920.10663851990086971No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT3820.10391814949523527TruSeq Adapter, Index 11 (95% over 23bp)
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3740.10174185317072773No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGTA200.001840307437.027
TATAGGA200.001840307437.02
GATTACG355.617421E-737.01
TCGGTAG200.001840307437.028
GGCTCGG200.001840307437.025
AGGCTCG200.001840307437.024
CTTATAC20850.034.8705021
TTATACA21950.033.123012
CCTTAGG352.3839222E-531.7142851
CTTGGGT352.3839222E-531.7142854
TATACAC23800.030.9369743
CTTGCAA303.5947576E-430.83333412
AGATTGC303.5947576E-430.83333420
GTATAGA303.5947576E-430.8333341
TTGCAAC303.5947576E-430.83333413
TGAGATT303.5947576E-430.83333418
TTGTCGC250.005491539429.637
GAGCGTT250.005491539429.69
GCAATCA250.005491539429.623
GCTCGGT250.005491539429.626
Produced by FastQC (version 0.11.5)