##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631197.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	367597
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.3138763374021	31.0	31.0	34.0	28.0	34.0
2	31.313944346662243	31.0	31.0	34.0	28.0	34.0
3	30.7690215099688	31.0	31.0	34.0	26.0	34.0
4	34.944550690021956	37.0	35.0	37.0	32.0	37.0
5	35.152128553823886	37.0	35.0	37.0	32.0	37.0
6	35.35231789160412	37.0	35.0	37.0	33.0	37.0
7	35.3464663748616	37.0	35.0	37.0	33.0	37.0
8	35.371216304812066	37.0	35.0	37.0	33.0	37.0
9	37.19229482286308	39.0	37.0	39.0	34.0	39.0
10	36.99823992034756	39.0	37.0	39.0	33.0	39.0
11	37.04304714129876	39.0	37.0	39.0	33.0	39.0
12	36.940815621455016	39.0	37.0	39.0	33.0	39.0
13	37.030729304102046	39.0	37.0	39.0	33.0	39.0
14	37.96932238293566	40.0	37.0	41.0	33.0	41.0
15	37.97675171451345	40.0	37.0	41.0	33.0	41.0
16	37.9746515885603	40.0	37.0	41.0	33.0	41.0
17	37.953212349393496	40.0	37.0	41.0	33.0	41.0
18	38.05211141549033	40.0	37.0	41.0	33.0	41.0
19	38.01326180572747	40.0	37.0	41.0	33.0	41.0
20	37.97119127740433	40.0	37.0	41.0	33.0	41.0
21	37.914433469261176	40.0	37.0	41.0	33.0	41.0
22	37.758466472794936	39.0	37.0	41.0	32.0	41.0
23	37.840404029412646	39.0	37.0	41.0	33.0	41.0
24	37.73717685400044	39.0	37.0	41.0	32.0	41.0
25	37.622246645103196	39.0	37.0	41.0	32.0	41.0
26	37.49774889348934	39.0	37.0	41.0	32.0	41.0
27	37.276267216544205	39.0	36.0	41.0	31.0	41.0
28	37.33638196176791	39.0	36.0	41.0	31.0	41.0
29	37.244849658729535	39.0	36.0	41.0	31.0	41.0
30	37.05192643030275	39.0	36.0	40.0	31.0	41.0
31	37.02096861508663	39.0	36.0	40.0	31.0	41.0
32	36.81611656243114	39.0	35.0	40.0	31.0	41.0
33	36.84908745174743	39.0	35.0	40.0	31.0	41.0
34	36.761045383939475	39.0	35.0	40.0	30.0	41.0
35	36.70017709611341	39.0	35.0	40.0	30.0	41.0
36	36.66460553268933	39.0	35.0	40.0	30.0	41.0
37	36.52313539011472	39.0	35.0	40.0	30.0	41.0
38	36.453583679953866	39.0	35.0	40.0	30.0	41.0
39	36.318906302282116	39.0	35.0	40.0	30.0	41.0
40	36.09910309387726	38.0	35.0	40.0	29.0	41.0
41	36.04961955619877	38.0	35.0	40.0	28.0	41.0
42	35.90650086915834	38.0	35.0	40.0	28.0	41.0
43	35.04687470245949	38.0	34.0	40.0	26.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	3.0
9	12.0
10	13.0
11	14.0
12	18.0
13	15.0
14	14.0
15	16.0
16	12.0
17	17.0
18	18.0
19	38.0
20	54.0
21	128.0
22	270.0
23	420.0
24	786.0
25	1283.0
26	2066.0
27	3155.0
28	4582.0
29	6168.0
30	8055.0
31	10096.0
32	12820.0
33	16326.0
34	21736.0
35	28440.0
36	38271.0
37	54895.0
38	84311.0
39	73541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.19452008585489	22.752906035685818	16.232722247461215	25.819851630998077
2	13.093686836399643	21.025198791067393	42.68233962736366	23.198774745169302
3	13.69053610339584	30.509226136230712	35.11943786265938	20.68079989771407
4	9.901604202428201	20.835861010835234	33.13900820735751	36.12352657937905
5	12.825186277363526	37.659175673359684	33.59902284295029	15.916615206326492
6	25.912888298870772	39.81153273829764	16.104048727274705	18.171530235556872
7	23.208568078629586	33.00434987227861	19.14623895189569	24.640843097196115
8	25.711580888853824	31.91973819155216	23.3565562286961	19.012124690897913
9	21.805128986362785	12.499557939809085	16.898668922760525	48.79664415106761
10	15.485708534074	27.55844035723904	30.156938168701053	26.798912939985907
11	30.08756872335737	21.261054905235895	24.320655500452943	24.33072087095379
12	19.679159514359476	27.256207205173055	23.44034363718964	29.62428964327783
13	32.22061115841533	18.122835605296018	25.672135517972126	23.984417718316525
14	22.479780846960125	23.310581968840875	23.911783828486087	30.29785335571291
15	27.090264610429355	26.141127375903505	20.392440634716824	26.376167378950317
16	27.604142580053697	23.618527898758696	20.492006191563043	28.28532332962456
17	19.655220254789892	32.3324183820869	21.203927126717574	26.80843423640563
18	25.601949961506758	20.39543304216302	23.656613084437577	30.346003911892645
19	25.11228328849256	24.80787384010207	23.512705489979517	26.567137381425855
20	24.29916457424843	21.434886574155936	25.248573845814846	29.01737500578079
21	25.611199220885915	22.78527844351287	26.945812941890168	24.65770939371105
22	19.939770999219252	35.556057312763706	23.457209933704572	21.04696175431247
23	23.44904882248767	21.00914860567415	26.70778053139716	28.834022040441027
24	24.358196612050698	27.950445732690966	26.127525523875335	21.56383213138301
25	22.9093273340098	30.77772669526683	23.999923829628642	22.31302214109473
26	27.53776554215622	23.829628642235924	26.327200711648896	22.305405103958957
27	23.17102696703183	32.28590004815056	24.994763286969153	19.54830969784846
28	18.84128542942407	21.82553176440504	32.033993748588806	27.29918905758208
29	20.71670878706845	26.373991082625807	26.079919041776726	26.829381088529015
30	24.22408235105292	23.577450305633615	28.49587999902067	23.702587344292798
31	24.02495123736048	20.848374714701155	32.213810232401244	22.912863815537126
32	20.31708637448075	32.02175208176345	26.95098164566087	20.710179898094925
33	22.13266158320117	22.50099973612407	30.15421779829542	25.212120882379345
34	19.145694877814563	28.19663925440088	31.03262540227477	21.625040465509784
35	20.29423526307342	27.59326109843116	33.53237376801225	18.580129870483162
36	28.36639036771247	22.710740294398484	23.242572708700017	25.68029662918903
37	21.387280092057335	26.19308645065112	28.284235181462307	24.135398275829235
38	21.07634175469332	21.162849533592492	30.112052057008082	27.6487566547061
39	26.027415892947985	22.208287880477805	26.2594634885486	25.504832738025605
40	19.176979137479357	22.18734102835442	37.28430863146326	21.35137120270296
41	23.035824557871802	23.168306596626195	24.93056254539618	28.86530630010582
42	23.50345623060036	21.92428121012957	32.501353384276804	22.070909174993268
43	23.95394956977342	20.97215156815752	31.55085596454813	23.523042897520927
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	5.0
2	9.0
3	52.5
4	96.0
5	96.0
6	168.0
7	240.0
8	286.0
9	332.0
10	466.5
11	601.0
12	601.0
13	950.0
14	1299.0
15	3320.0
16	5341.0
17	5291.0
18	5241.0
19	5241.0
20	4324.0
21	3407.0
22	2705.0
23	2003.0
24	2129.5
25	2256.0
26	2256.0
27	2595.5
28	2935.0
29	3514.0
30	4093.0
31	5236.0
32	6379.0
33	6379.0
34	8169.5
35	9960.0
36	11215.5
37	12471.0
38	14262.0
39	16053.0
40	16053.0
41	17927.5
42	19802.0
43	21656.5
44	23511.0
45	25531.5
46	27552.0
47	27552.0
48	31247.5
49	34943.0
50	48300.5
51	61658.0
52	45209.5
53	28761.0
54	28761.0
55	37082.5
56	45404.0
57	36464.0
58	27524.0
59	19059.5
60	10595.0
61	10595.0
62	8669.0
63	6743.0
64	5300.5
65	3858.0
66	3051.5
67	2245.0
68	2245.0
69	1731.5
70	1218.0
71	927.0
72	636.0
73	408.5
74	181.0
75	181.0
76	135.0
77	89.0
78	71.5
79	54.0
80	41.5
81	29.0
82	29.0
83	26.5
84	24.0
85	24.5
86	25.0
87	22.0
88	19.0
89	19.0
90	12.5
91	6.0
92	4.5
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	367597.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	19.288242287069806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.26372649958394	12.97399053855173
2	11.292893107484874	4.356401167582978
3	5.019533729179301	2.904539482095882
4	3.0943683624106173	2.387397067984777
5	2.0309436836241064	1.9586666920567906
6	1.4808964359759107	1.7138333555496919
7	1.19176903657109	1.6090990949327661
8	0.8504576675175944	1.3123066836780497
9	0.7742972793816905	1.3441350174239726
>10	5.80511402902557	22.575809922279017
>50	0.753141616010606	9.96770920328512
>100	0.4061887367248212	14.486516484084472
>500	0.015514153138795254	2.1795607689943064
>1k	0.015514153138795254	6.67551693838633
>5k	0.001410377558072296	1.9097000247553706
>10k+	0.004231132674216888	11.644817558358746
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGT	17507	4.7625524691441985	No Hit
ATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATG	13894	3.7796826415884786	No Hit
TCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTT	11405	3.1025824476260686	TruSeq Adapter, Index 11 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCC	7020	1.9097000247553706	No Hit
CTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTC	4691	1.2761257572831117	RNA PCR Primer, Index 11 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3585	0.9752527904199436	No Hit
CATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTC	3464	0.9423363085117671	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3237	0.8805839003038654	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1922	0.5228551919629376	No Hit
CACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCG	1529	0.4159446350215045	No Hit
TTATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTA	1435	0.3903731532085409	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1310	0.35636852313811046	No Hit
TATACACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTAT	1189	0.3234520412299339	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	1124	0.30576963359331005	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1053	0.2864550037133056	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	1000	0.27203704056344313	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	877	0.2385764845741396	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	805	0.2189898176535717	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	799	0.21735759541019103	No Hit
ACATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGT	792	0.21545333612624692	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	763	0.2075642619499071	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	706	0.19205815063779083	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	692	0.18824963206990264	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	547	0.1488042611882034	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	522	0.1420033351741173	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	509	0.13846685364679256	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	496	0.1349303721194678	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	492	0.133842223957214	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	490	0.13329814987608712	No Hit
GTATTAGAGGCACCGCCTGCCCAGTGACACATGTTTAACGGCC	486	0.13221000171383335	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	481	0.13084981651101613	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	459	0.12486500161862038	No Hit
GTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA	442	0.12024037192904186	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	440	0.11969629784791497	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	435	0.11833611264509775	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.11588777928002678	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	404	0.10990296438763102	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	399	0.1085427791848138	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	399	0.1085427791848138	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	398	0.10827074214425035	No Hit
ATCTCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCT	394	0.1071825939819966	No Hit
GTACATGCTAAGACTTCACCAGTCAAAGCGAACTACTATACTC	392	0.10663851990086971	No Hit
TCCGAGCCCACGAGACCGTACTAGATCTCGTATGCCGTCTTCT	382	0.10391814949523527	TruSeq Adapter, Index 11 (95% over 23bp)
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	374	0.10174185317072773	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.720370405634431E-4	0.0
11	0.0	0.0	0.0	5.440740811268862E-4	0.0
12	0.0	0.0	0.0	5.440740811268862E-4	0.0
13	0.0	0.0	0.0	5.440740811268862E-4	0.0
14	0.0	0.0	0.0	5.440740811268862E-4	0.0
15	0.0	0.0	0.0	5.440740811268862E-4	0.0
16	0.0	0.0	0.0	8.161111216903294E-4	0.0
17	0.0	0.0	0.0	0.0010881481622537724	0.0
18	0.0	0.0	0.0	0.0010881481622537724	0.0
19	0.0	0.0	0.0	0.0010881481622537724	0.0
20	0.0	0.0	0.0	0.0010881481622537724	0.0
21	0.0	0.0	0.0	0.0016322222433806587	0.0
22	0.0	0.0	0.0	0.002448333365070988	0.0
23	0.0	0.0	0.0	0.003808518567888204	0.0
24	0.0	0.0	0.0	0.005168703770705419	0.0
25	0.0	0.0	0.0	0.006256851932959192	0.0
26	0.0	0.0	0.0	0.007345000095212964	0.0
27	0.0	0.0	0.0	0.008977222338593624	0.0
28	0.0	0.0	0.0	0.021490926204512005	0.0
29	0.0	0.0	0.0	0.04624629689578533	0.0
30	0.0	0.0	0.0	0.07916277880396194	0.0
31	0.0	0.0	0.0	0.136290557322285	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGGTA	20	0.0018403074	37.0	27
TATAGGA	20	0.0018403074	37.0	2
GATTACG	35	5.617421E-7	37.0	1
TCGGTAG	20	0.0018403074	37.0	28
GGCTCGG	20	0.0018403074	37.0	25
AGGCTCG	20	0.0018403074	37.0	24
CTTATAC	2085	0.0	34.870502	1
TTATACA	2195	0.0	33.12301	2
CCTTAGG	35	2.3839222E-5	31.714285	1
CTTGGGT	35	2.3839222E-5	31.714285	4
TATACAC	2380	0.0	30.936974	3
CTTGCAA	30	3.5947576E-4	30.833334	12
AGATTGC	30	3.5947576E-4	30.833334	20
GTATAGA	30	3.5947576E-4	30.833334	1
TTGCAAC	30	3.5947576E-4	30.833334	13
TGAGATT	30	3.5947576E-4	30.833334	18
TTGTCGC	25	0.0054915394	29.6	37
GAGCGTT	25	0.0054915394	29.6	9
GCAATCA	25	0.0054915394	29.6	23
GCTCGGT	25	0.0054915394	29.6	26
>>END_MODULE