FastQCFastQC Report
Fri 10 Feb 2017
ERR1631196.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631196.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences867698
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA67260.7751544892347337No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT36730.42330396059458475No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35240.4061320874313413No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34590.39864100182321505No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG33640.3876924920882611No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC22360.2576933449195457No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT21210.24443988576670683No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC20220.23303038614817598No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA19650.22646128030720364No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG18290.21078762426558548No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG17000.19592070052022711No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA16500.19015832697551452No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT12090.13933419231114974No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC11850.1365682530096877No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA11270.12988389969782113No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG11190.12896191993066713No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11060.12746370280904185No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC10770.12412152615310858No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG10430.12020311214270402No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC9720.11202054170921219No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT9060.10441420863019162No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATTACG303.598757E-430.8333341
GGTATCA4200.028.1904771
AGGGGCG358.867638E-426.428575
CCGTCTT3650.026.35616537
GCCGTCT3850.024.98701336
GTATCAA13900.024.6223031
TATACAC7200.023.8958323
CTTATAC7000.023.7857131
TTATACA7400.023.52
GTCGGTG400.001930807823.12532
GCTTTAT11800.022.889831
CGTCTTC1155.456968E-1222.5217437
CACGTAC502.7013622E-422.224
CCGCCGT502.7013622E-422.224
ATTGAGC12650.021.4980226
TATTGAG13150.020.9619775
TTATTGA13450.020.7695164
CGCGGCT450.003825063620.55555510
GCTCTAT909.47166E-820.5555551
TTGAGCC13400.020.4328377
Produced by FastQC (version 0.11.5)