##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631196.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	867698
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.583323921456543	31.0	31.0	34.0	30.0	34.0
2	31.56450400945951	31.0	31.0	34.0	30.0	34.0
3	31.044710256333424	31.0	31.0	34.0	27.0	34.0
4	35.19245636154515	37.0	35.0	37.0	32.0	37.0
5	35.395538539906745	37.0	35.0	37.0	33.0	37.0
6	35.58761112737381	37.0	35.0	37.0	33.0	37.0
7	35.606014996000916	37.0	35.0	37.0	33.0	37.0
8	35.623229510728386	37.0	35.0	37.0	33.0	37.0
9	37.389806130704464	39.0	37.0	39.0	34.0	39.0
10	37.24924455282829	39.0	37.0	39.0	34.0	39.0
11	37.29186076261556	39.0	37.0	39.0	34.0	39.0
12	37.244681905455586	39.0	37.0	39.0	34.0	39.0
13	37.31032686487695	39.0	37.0	39.0	34.0	39.0
14	38.50886022556235	40.0	38.0	41.0	34.0	41.0
15	38.51177598657598	40.0	38.0	41.0	34.0	41.0
16	38.51665671696835	40.0	38.0	41.0	34.0	41.0
17	38.509956229010555	40.0	38.0	41.0	34.0	41.0
18	38.522925026910286	40.0	38.0	41.0	34.0	41.0
19	38.556607252753835	40.0	38.0	41.0	34.0	41.0
20	38.55188786882072	40.0	38.0	41.0	34.0	41.0
21	38.5146145317841	40.0	38.0	41.0	34.0	41.0
22	38.466415734506704	40.0	38.0	41.0	34.0	41.0
23	38.464610959112505	40.0	38.0	41.0	34.0	41.0
24	38.432508776094906	40.0	38.0	41.0	34.0	41.0
25	38.40684201185205	40.0	38.0	41.0	34.0	41.0
26	38.31396638000779	40.0	38.0	41.0	34.0	41.0
27	38.24674714013401	40.0	38.0	41.0	34.0	41.0
28	38.199799930390526	40.0	38.0	41.0	34.0	41.0
29	38.13319841696074	40.0	38.0	41.0	33.0	41.0
30	38.07014191573566	40.0	37.0	41.0	33.0	41.0
31	38.03113756168621	40.0	37.0	41.0	33.0	41.0
32	37.94872524772444	40.0	37.0	41.0	33.0	41.0
33	37.94938792068208	40.0	37.0	41.0	33.0	41.0
34	37.890815698549495	40.0	37.0	41.0	33.0	41.0
35	37.83209365470475	40.0	37.0	41.0	33.0	41.0
36	37.81977370006615	40.0	37.0	41.0	33.0	41.0
37	37.757239269884224	40.0	37.0	41.0	33.0	41.0
38	37.721280906490506	40.0	37.0	41.0	33.0	41.0
39	37.649500171718735	40.0	37.0	41.0	33.0	41.0
40	37.58206311412496	40.0	37.0	41.0	32.0	41.0
41	37.54228083964697	40.0	37.0	41.0	32.0	41.0
42	37.48406242725003	40.0	37.0	41.0	32.0	41.0
43	36.92271158859419	39.0	36.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	5.0
11	3.0
12	6.0
13	4.0
14	3.0
15	2.0
16	3.0
17	9.0
18	8.0
19	16.0
20	55.0
21	91.0
22	235.0
23	444.0
24	890.0
25	1464.0
26	2483.0
27	3928.0
28	5873.0
29	8424.0
30	12188.0
31	16761.0
32	22320.0
33	29419.0
34	40055.0
35	54199.0
36	77218.0
37	117050.0
38	208585.0
39	265954.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.16939073272037	22.085333837348937	14.967189045036406	22.778086384894284
2	14.231103448434823	22.616048440816964	39.33453805356241	23.818310057185794
3	15.387727066329528	27.909825768873503	33.689601681691094	23.012845483105874
4	11.964300943415797	17.837427307657734	41.18875461278002	29.009517136146446
5	12.624668951639856	38.28809101784261	36.46994691701491	12.617293113502623
6	28.22041770293351	41.20604173341416	17.424956609327207	13.148583954325122
7	25.14849636624724	32.39664030572849	24.579865344855005	17.874997983169262
8	25.296474118875462	34.264571313982515	22.248178513722515	18.19077605341951
9	25.203354162392905	15.622370917070224	20.715502398299872	38.458772522237
10	14.650258500077214	29.435471788571597	34.152896514685985	21.761373196665197
11	32.59843862726433	24.45516758134743	25.138008846395866	17.80838494499238
12	22.356972126246692	26.287487121095126	30.062879020119905	21.292661732538278
13	28.27089609518519	21.63367899891437	26.776136397686752	23.31928850821369
14	21.223974239885308	21.527766573162552	28.919854603790718	28.328404583161422
15	23.223287364958775	30.18077718284472	24.203236609972596	22.392698842223908
16	22.509675025181572	28.312269937236227	25.75089489661149	23.427160140970706
17	22.4469804010151	28.653978688437682	26.36516391647785	22.533876994069367
18	22.66134069687841	27.012278465549073	28.78812674455859	21.53825409301393
19	24.68185935659642	26.721163354070192	28.215116319272372	20.381860970061012
20	24.50022934246708	26.635534483195766	26.823733603166076	22.040502571171075
21	23.584357691270466	26.51682958817469	27.924231702735288	21.974581017819563
22	21.789147837150715	28.73845508460317	28.332322997171826	21.140074081074292
23	23.232046172746738	26.33128116003494	27.75931257188561	22.67736009533271
24	21.9177640146687	27.362515529596703	28.406888110840406	22.31283234489419
25	22.25152069037845	28.312500432178016	27.488250520342334	21.947728357101205
26	23.55543057607601	27.039361621209224	27.749747031801387	21.655460770913383
27	22.40675903367301	27.13340355745893	28.012626512911176	22.447210895956886
28	22.84850258961067	26.699957819425652	28.04939045612644	22.402149134837234
29	22.728299477467967	28.174203467104913	27.95419604516779	21.14330101025933
30	23.48075021493653	26.671261199172985	27.02437944999297	22.823609135897513
31	22.751118476705027	27.08050496831847	27.708718932163034	22.459657622813467
32	21.59679980822821	28.36758872326547	28.339122597954585	21.696488870551736
33	20.996821474752736	27.833992933025087	28.35306754193279	22.816118050289386
34	23.792033633821905	26.682670698791515	27.708718932163034	21.816576735223546
35	21.857835329803688	26.72750196496938	28.665964425410685	22.74869827981625
36	22.005121597606543	27.406540063478307	28.054115602433104	22.53422273648205
37	22.620427844710946	26.612715483958706	28.97079398592598	21.79606268540437
38	22.902323158518286	26.40250409704759	28.282766584687298	22.412406159746826
39	23.333118204721	26.90452208026295	27.7334971384053	22.028862576610756
40	22.150794400816874	26.14792243384219	29.27147463748908	22.429808527851854
41	21.72829717251855	26.595313115853674	27.766918904964633	23.909470806663148
42	22.12520946227835	26.670569714347618	28.35410476917084	22.85011605420319
43	22.701907806633184	27.05353706012922	28.381303172301887	21.863251960935717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	30.0
1	42.0
2	54.0
3	205.5
4	357.0
5	357.0
6	416.5
7	476.0
8	431.0
9	386.0
10	624.0
11	862.0
12	862.0
13	1305.0
14	1748.0
15	3921.0
16	6094.0
17	6976.0
18	7858.0
19	7858.0
20	8436.5
21	9015.0
22	11235.0
23	13455.0
24	17040.5
25	20626.0
26	20626.0
27	24437.5
28	28249.0
29	31675.0
30	35101.0
31	38973.0
32	42845.0
33	42845.0
34	45966.5
35	49088.0
36	51809.0
37	54530.0
38	56013.0
39	57496.0
40	57496.0
41	57515.0
42	57534.0
43	57401.0
44	57268.0
45	57871.5
46	58475.0
47	58475.0
48	58800.0
49	59125.0
50	56241.5
51	53358.0
52	54179.0
53	55000.0
54	55000.0
55	49849.0
56	44698.0
57	41479.0
58	38260.0
59	36568.0
60	34876.0
61	34876.0
62	31003.5
63	27131.0
64	24289.0
65	21447.0
66	17454.5
67	13462.0
68	13462.0
69	11519.0
70	9576.0
71	8212.5
72	6849.0
73	4155.5
74	1462.0
75	1462.0
76	966.0
77	470.0
78	354.5
79	239.0
80	178.0
81	117.0
82	117.0
83	83.0
84	49.0
85	36.0
86	23.0
87	15.5
88	8.0
89	8.0
90	4.5
91	1.0
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	867698.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.94317003419962
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.41101058580189	43.10156976100134
2	11.699551699302448	12.388227098801572
3	2.960231279088523	4.701720838480197
4	1.148636673707127	2.4324986689437558
5	0.6210867962705656	1.6441151930474427
6	0.36797400104995853	1.1689026063451622
7	0.23246499549874566	0.8615203648582678
8	0.1874273206367343	0.793839720442006
9	0.141065208140167	0.672159537042716
>10	1.0078558045429067	10.648122070557438
>50	0.11517306819898779	4.218111627486673
>100	0.09484714311112716	9.539246164531685
>500	0.008523130368698817	2.9548221684721323
>1k	0.0039337524778609925	4.0969260773525615
>5k	2.185418043256107E-4	0.7782181026369943
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	6726	0.7751544892347337	No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGT	3673	0.42330396059458475	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3524	0.4061320874313413	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3459	0.39864100182321505	No Hit
ATACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATG	3364	0.3876924920882611	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	2236	0.2576933449195457	No Hit
TCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTCTT	2121	0.24443988576670683	No Hit
ACACATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCC	2022	0.23303038614817598	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	1965	0.22646128030720364	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	1829	0.21078762426558548	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	1700	0.19592070052022711	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	1650	0.19015832697551452	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	1209	0.13933419231114974	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	1185	0.1365682530096877	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	1127	0.12988389969782113	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	1119	0.12896191993066713	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1106	0.12746370280904185	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	1077	0.12412152615310858	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	1043	0.12020311214270402	No Hit
CATCTCCGAGCCCACGAGACTCGACGTCATCTCGTATGCCGTC	972	0.11202054170921219	No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT	906	0.10441420863019162	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	1.1524747089425122E-4	0.0
5	0.0	0.0	0.0	1.1524747089425122E-4	0.0
6	0.0	0.0	0.0	1.1524747089425122E-4	0.0
7	0.0	0.0	0.0	1.1524747089425122E-4	0.0
8	0.0	0.0	0.0	2.3049494178850244E-4	0.0
9	0.0	0.0	0.0	4.609898835770049E-4	0.0
10	0.0	0.0	0.0	4.609898835770049E-4	0.0
11	1.1524747089425122E-4	0.0	0.0	4.609898835770049E-4	0.0
12	1.1524747089425122E-4	0.0	0.0	4.609898835770049E-4	0.0
13	1.1524747089425122E-4	0.0	0.0	6.914848253655074E-4	0.0
14	1.1524747089425122E-4	0.0	0.0	6.914848253655074E-4	0.0
15	2.3049494178850244E-4	0.0	0.0	8.067322962597586E-4	0.0
16	2.3049494178850244E-4	0.0	0.0	8.067322962597586E-4	0.0
17	2.3049494178850244E-4	0.0	0.0	8.067322962597586E-4	0.0
18	2.3049494178850244E-4	0.0	0.0	8.067322962597586E-4	0.0
19	2.3049494178850244E-4	0.0	0.0	9.219797671540098E-4	0.0
20	2.3049494178850244E-4	0.0	0.0	9.219797671540098E-4	0.0
21	2.3049494178850244E-4	0.0	0.0	0.001037227238048261	0.0
22	2.3049494178850244E-4	0.0	0.0	0.0012677221798367635	0.0
23	2.3049494178850244E-4	0.0	0.0	0.0013829696507310148	0.0
24	2.3049494178850244E-4	0.0	0.0	0.0016134645925195172	0.0
25	2.3049494178850244E-4	0.0	0.0	0.0018439595343080195	0.0
26	2.3049494178850244E-4	0.0	0.0	0.002074454476096522	0.0
27	3.457424126827537E-4	0.0	0.0	0.0023049494178850245	0.0
28	3.457424126827537E-4	0.0	0.0	0.007721580549914832	0.0
29	3.457424126827537E-4	0.0	0.0	0.019131080168445704	0.0
30	3.457424126827537E-4	0.0	0.0	0.03388275644290986	0.0
31	3.457424126827537E-4	0.0	0.0	0.07548709343573455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GATTACG	30	3.598757E-4	30.833334	1
GGTATCA	420	0.0	28.190477	1
AGGGGCG	35	8.867638E-4	26.42857	5
CCGTCTT	365	0.0	26.356165	37
GCCGTCT	385	0.0	24.987013	36
GTATCAA	1390	0.0	24.622303	1
TATACAC	720	0.0	23.895832	3
CTTATAC	700	0.0	23.785713	1
TTATACA	740	0.0	23.5	2
GTCGGTG	40	0.0019308078	23.125	32
GCTTTAT	1180	0.0	22.88983	1
CGTCTTC	115	5.456968E-12	22.52174	37
CACGTAC	50	2.7013622E-4	22.2	24
CCGCCGT	50	2.7013622E-4	22.2	24
ATTGAGC	1265	0.0	21.498022	6
TATTGAG	1315	0.0	20.961977	5
TTATTGA	1345	0.0	20.769516	4
CGCGGCT	45	0.0038250636	20.555555	10
GCTCTAT	90	9.47166E-8	20.555555	1
TTGAGCC	1340	0.0	20.432837	7
>>END_MODULE