FastQCFastQC Report
Fri 10 Feb 2017
ERR1631195.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631195.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences438910
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGT236565.389715431409629No Hit
ATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATG171253.9017110569365023No Hit
TCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTT133433.0400309858513137TruSeq Adapter, Index 1 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCC82751.88535235014012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT82301.8750996787496297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT76891.7518397849217378No Hit
CTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCTTC55081.2549269781959855RNA PCR Primer, Index 1 (95% over 23bp)
CATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTC43961.0015720762798752No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA36610.8341117769018704No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30690.6992321888314234No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA22440.5112665466724385No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA21200.48301474106308806No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT19930.454079424027705No Hit
TTATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTA19360.4410927069330843No Hit
CACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCG17810.4057779499213962No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT16740.3813993757262309No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT16620.37866533002210023No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG15880.3618053815132943No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC14940.3403886901642706No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC14020.3194276730992686No Hit
TATACACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTAT13930.31737713882117063No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG13340.3039347474425281No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG12060.27477159326513406No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11110.2531270647740995No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG11010.25084869335399057No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG10700.24378574195165295No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT10590.24127953338953317No Hit
ACATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGT10200.2323938848511084No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG9440.21507826205828073No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9330.21257205349616096No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9120.20778747351393223No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG8950.20391424209974712No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG8890.20254721924768174No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8870.20209154496366No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG8750.1993574992595293No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG8710.19844615069148572No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA8690.19799047640746392No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG8520.1941172449932788No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8030.18295322503474515No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7890.1797635050465927No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC7640.17406757649632043No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA7530.17156136793420065No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC7390.16837164794604814No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG7030.1601695108336561No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7020.15994167369164522No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG6940.1581189765555581No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC6710.1528787222893076No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC6660.15173953657925315No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6470.14741063088104622No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT6360.14490442231892645No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG6340.14444874803490465No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT6330.14422091089289377No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT6330.14422091089289377No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA6310.143765236608872No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA6140.13989200519468684No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC6010.13693012234854526No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5880.13396823950240366No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA5870.1337404023603928No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC5810.13237337950832745No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5660.12895582237816408No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA5540.1262217766740334No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG5530.1259939395320225No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA5510.12553826524800074No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG5320.12120935954979381No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG5230.1191588252716958No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG5080.11574126814153243No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC5070.11551343099952154No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC4960.11300722243740174No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4940.11255154815337998No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4940.11255154815337998No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4930.11232371101136908No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4920.11209587386935818No Hit
ATCTCCGAGCCCACGAGACATCTCAGGATCTCGTATGCCGTCT4820.10981750244924927RNA PCR Primer, Index 40 (95% over 22bp)
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4800.1093618281652275No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4770.10867831673919481No Hit
ATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAAAT4740.10799480531316216No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT4590.1045772481829988No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC4540.10343806247294435No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC4540.10343806247294435No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA4520.10298238818892255No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4510.10275455104691168No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA4490.10229887676288989No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT4430.10093185391082454No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG4410.10047617962680278No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGA200.001840696637.01
TTCTACA308.300272E-637.02
CTAGAAG200.001840696637.030
GTCGACG200.001840696637.036
ACGTTTA355.620732E-737.026
ACGCATC200.001840696637.01
CGCATCT200.001840696637.02
TGGCAGG200.001840696637.012
ACTAGAA200.001840696637.029
GCCTCGC200.001840696637.035
ACACTAG200.001840696637.027
CACGTTT355.620732E-737.025
ACTCAGA950.037.01
CGCCGAG200.001840696637.02
TCGACGA200.001840696637.037
CTTAATA251.232383E-436.9999961
CTTATAC27200.035.0275731
GATACAC603.45608E-1133.9166683
TTACGGG1050.033.4761923
TCAGAGT950.033.1052633
Produced by FastQC (version 0.11.5)