FastQCFastQC Report
Fri 10 Feb 2017
ERR1631194.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631194.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences583928
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGT173352.968687920428546No Hit
ATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATG124242.127659574468085No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT116141.9889438423915278No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT110141.8861914482607445No Hit
TCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTT80091.3715732076557383RNA PCR Primer, Index 29 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCC67341.153224370127824No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA54010.9249428011672672No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT40080.6863859927936321No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA39000.6678905618500911No Hit
CATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTC34880.5973339178802866RNA PCR Primer, Index 29 (95% over 21bp)
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT29580.5065693030647614No Hit
CTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGTCTTC27120.46444082147114024RNA PCR Primer, Index 29 (95% over 24bp)
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA25450.4358414051047389No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT24430.4183734981025058No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG23490.40227562302201636No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT22830.39097285966763023No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC22030.3772725404501925No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC19800.33908290063158475No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG19290.33034894713046814No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG17610.30157827677384885No Hit
CACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCG15800.2705813045443959No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG15210.26047731912153554No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT15160.25962104917044565No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14730.2522571275910729No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG14490.24814703182584152No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG14420.24694825389431574No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG14270.24437944404104617No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG13950.23889931635407102No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG13710.23478922058883972No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG13620.23324793467687796No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC13250.22691153703881303No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG13250.22691153703881303No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA13120.22468523516597938No Hit
TTATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTA13020.22297269526379962No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG12630.21629378964529875No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA11660.19968215259415545No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC10990.18820813524955132No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC10950.18752311928867943No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG10830.18546807140606378No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT10520.18015919770930663No Hit
TATACACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTAT10500.1798166897288707No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT10420.1784466578071269No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG10160.17399405406145965No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG10110.17313778411036976No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA9820.1681714183940486No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT9790.16765765642339467No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA9190.15738241701031636No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA9130.1563548930690085No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG9040.15481360715704676No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8990.1539573372059569No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC8270.1416270499102629No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT8250.14128454192982698No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG8140.13940074803742927No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA8080.13837322409612143No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG7890.13511939828197997No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG7860.13460563631132605No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG7790.13340685837980024No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC7760.13289309640914634No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7750.13272184241892834No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC7690.13169431847762053No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG7600.13015303256565877No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG7540.12912550862435096No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7520.128783000643915No Hit
ACATCTCCGAGCCCACGAGACTGCAGCTAATCTCGTATGCCGT7200.1233028729569399No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT7200.1233028729569399No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA7190.12313161896672194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC7000.11987779315258044No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA6970.11936403118192654No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6950.11902152320149059No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA6880.1178227452699648No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG6760.11576769738734913No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA6580.11268512556342562No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC6570.11251387157320766No Hit
CTGTGAGCTCGATGAGTAGGGCGGGACACGTGGTATCCTGTCT6370.10908879176884821No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC6350.10874628378841228No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG6340.1085750297981943No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC6300.10789001383732241No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC6280.10754750585688647No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6260.10720499787645052No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA6240.10686248989601457No Hit
ATTGTAGCACGTGTGTAGCCCTGGTCGTAAGGGCCATGATGAC6220.10651998191557864No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA6200.10617747393514268No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA6090.104293680042745No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG6050.10360866408187311No Hit
CCCCAGTAGCGGCGAGCGAACGGGGAGCAGCCCAGAGCCTGAA5990.10258114014056528No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA5950.10189612417969339No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT5870.1005260922579496No Hit
AGCTCACAGCATGTGCATTTTTGTGTACGGGGCTGTCACCCTG5860.10035483826773164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGG1050.037.02
TTACTTT501.8007995E-1037.03
GATTAGG355.62497E-737.01
TTACGGG1150.035.39133
CTTATAC20400.033.100491
CGTCTTC3450.032.17391237
CATATAT352.38631E-531.7142872
TTATACA21400.031.467292
ACTCAGA1000.031.451
TATACAC21500.031.4069753
GATTACG1350.030.148151
GATCGCA250.00549415629.610
GTGATCG250.00549415629.68
ACAATCT250.00549415629.636
ATACGGG250.00549415629.63
TTTACAC454.0038194E-628.7777772
TCAGAGT1100.028.5909083
TTATCAA1000.027.7500022
ATATATA405.9352016E-527.753
ACATATA405.9352016E-527.751
Produced by FastQC (version 0.11.5)