FastQCFastQC Report
Fri 10 Feb 2017
ERR1631193.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631193.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences907568
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT191922.1146624825908362No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG142071.565392345256774No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT126541.394275690636955TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC71020.7825308957565714No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC53390.5882754790825592RNA PCR Primer, Index 8 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36970.40735239673501056No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36510.40228390599933006No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC35190.38773954127955146No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA25790.28416603494173437No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG16270.17927031362939197No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA14920.16439539516598206No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14310.15767413571214497No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13180.14522327803536483No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT11240.12384746928053875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23700.033.0970461
GATTACG800.032.3751
TTATACA25100.030.9561752
CGTCTTC6850.030.7883237
TATACAC26050.030.3243753
CCGTCTT20050.024.72818237
GGTATCA6500.024.1923071
GCCGTCT20850.023.77937736
GTATCAA18000.023.3305551
AATGCCG400.00193087623.1255
GCTTTAT5250.021.847621
CGAATGA603.725174E-521.58333410
GTTACGG450.003825197220.55555517
TGCCGTC24450.020.27811835
TTACGGG1202.382876E-1020.0416683
ATTGAGC5800.019.1379326
ATGCCGT26200.018.92366434
ATACACA43300.018.7136274
TACACAT43550.018.691165
AGCAACG500.007033769518.52
Produced by FastQC (version 0.11.5)