##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631193.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	907568
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.409340126580048	31.0	31.0	34.0	28.0	34.0
2	31.398554157925357	31.0	31.0	34.0	28.0	34.0
3	30.875914532024048	31.0	31.0	34.0	27.0	34.0
4	35.03467178216949	37.0	35.0	37.0	32.0	37.0
5	35.23189997884456	37.0	35.0	37.0	33.0	37.0
6	35.42778943285792	37.0	35.0	37.0	33.0	37.0
7	35.43384517744125	37.0	35.0	37.0	33.0	37.0
8	35.46187062567213	37.0	35.0	37.0	33.0	37.0
9	37.236479250039665	39.0	37.0	39.0	34.0	39.0
10	37.06242066710153	39.0	37.0	39.0	33.0	39.0
11	37.12065321827125	39.0	37.0	39.0	33.0	39.0
12	37.04791156144774	39.0	37.0	39.0	33.0	39.0
13	37.13545982229431	39.0	37.0	39.0	33.0	39.0
14	38.22540790331964	40.0	38.0	41.0	33.0	41.0
15	38.23172037797719	40.0	38.0	41.0	33.0	41.0
16	38.23161129524179	40.0	38.0	41.0	33.0	41.0
17	38.197051901345134	40.0	38.0	41.0	33.0	41.0
18	38.255373702025636	40.0	38.0	41.0	33.0	41.0
19	38.28673443752975	40.0	38.0	41.0	34.0	41.0
20	38.28387074026409	40.0	38.0	41.0	34.0	41.0
21	38.245309442377874	40.0	38.0	41.0	34.0	41.0
22	38.166157246619534	40.0	38.0	41.0	33.0	41.0
23	38.165187622304884	40.0	38.0	41.0	33.0	41.0
24	38.12088460589179	40.0	37.0	41.0	33.0	41.0
25	38.076053805334695	40.0	37.0	41.0	33.0	41.0
26	37.95475600726337	40.0	37.0	41.0	33.0	41.0
27	37.88325502882429	40.0	37.0	41.0	33.0	41.0
28	37.86343723004778	40.0	37.0	41.0	33.0	41.0
29	37.81777343405673	40.0	37.0	41.0	33.0	41.0
30	37.71912187296159	40.0	37.0	41.0	33.0	41.0
31	37.68107403522381	40.0	37.0	41.0	33.0	41.0
32	37.59529533875148	40.0	37.0	41.0	32.0	41.0
33	37.505903689861256	39.0	37.0	41.0	32.0	41.0
34	37.46216481850396	39.0	36.0	41.0	32.0	41.0
35	37.43380661283783	39.0	36.0	41.0	32.0	41.0
36	37.380810032967226	39.0	36.0	41.0	32.0	41.0
37	37.324717266364615	39.0	36.0	41.0	31.0	41.0
38	37.29378074149816	39.0	36.0	41.0	31.0	41.0
39	37.17877668670557	39.0	36.0	41.0	31.0	41.0
40	37.07208716041112	39.0	36.0	41.0	31.0	41.0
41	37.02840007580699	39.0	36.0	41.0	31.0	41.0
42	36.95943664827319	39.0	36.0	41.0	31.0	41.0
43	36.288130476173684	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	2.0
9	8.0
10	15.0
11	22.0
12	17.0
13	22.0
14	9.0
15	12.0
16	16.0
17	22.0
18	25.0
19	47.0
20	89.0
21	196.0
22	368.0
23	582.0
24	1276.0
25	2174.0
26	3653.0
27	5554.0
28	7999.0
29	11558.0
30	15553.0
31	20814.0
32	26985.0
33	35153.0
34	46496.0
35	62556.0
36	86476.0
37	127707.0
38	218911.0
39	233247.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.11273645611128	22.20902455793946	15.354992683743806	24.323246302205455
2	13.59964212048023	22.017303386633287	41.07482855279164	23.308225940094847
3	14.55362022460025	28.54144262468487	34.622860215432894	22.28207693528198
4	11.17668317966257	19.396012199636832	38.375196128554556	31.05210849214604
5	12.490524126015902	38.03516651093913	35.84117112987677	13.633138233168204
6	27.1247994640622	41.05422403610529	17.283443224088995	14.537533275743526
7	24.243582849990304	33.20555594732296	22.84489977610493	19.705961426581812
8	24.95724838249035	34.50154699152019	22.47512032156268	18.066084304426774
9	23.76273733758793	14.791178181689968	19.67389771344957	41.77218676727254
10	14.447843026638225	29.085864640445674	33.305934100805665	23.160358232110433
11	31.151494984397864	23.247954974172735	25.451316044637977	20.149233996791423
12	20.596142658180984	26.73893306066322	28.637193025756748	24.027731255399047
13	29.16585864640446	20.58292050843573	26.71524337570298	23.535977469456835
14	21.57127620189341	22.417163231845986	27.0214463268868	28.9901142393738
15	23.955339985543784	29.131260687904376	22.87431905928812	24.03908026726372
16	24.507034183664477	27.333599245455986	23.955339985543784	24.204026585335754
17	21.082277030481464	30.641891296299562	24.866786841316575	23.409044831902403
18	24.205789538635123	25.316560301817603	26.406946917476155	24.07070324207112
19	25.141146448530577	26.600100488338065	25.974031697900323	22.284721365231036
20	22.631912980625142	25.452968813356136	26.19186661495337	25.723251591065356
21	24.102987324365778	25.359311919327254	27.294042980801436	23.24365777550553
22	20.744671473652662	30.59726654090933	26.322435343687744	22.33562664175026
23	22.925665074132187	26.398462704722952	27.20016571761014	23.47570650353472
24	24.008559138268424	27.10000793328985	27.412271036440245	21.479161892001482
25	22.601942774535903	29.004548419512368	25.982075172328685	22.411433633623044
26	24.94788269308746	27.730263737813587	26.90531177829099	20.416541790807962
27	21.410957636232215	28.846874283800222	26.549966503887312	23.192201576080247
28	20.87072263455741	26.493662182888777	29.49354759092432	23.1420675916295
29	23.6885831144333	25.843242600003524	27.00602048551734	23.462153800045837
30	22.066115156109515	27.075436771679918	27.403786823687042	23.454661248523525
31	21.750546515522803	25.958716041112073	29.848231757840736	22.44250568552439
32	23.28629920843397	26.303924334044392	27.248426564180317	23.161349893341328
33	20.148683073885373	29.997421680799675	28.810072633675933	21.04382261163902
34	21.666255310896815	27.82843819967209	28.968187507712923	21.537118981718173
35	23.33577208539746	25.771402253054315	30.196194665303317	20.69663099624491
36	22.53682368704053	28.211990726865643	25.90108950513901	23.350096080954817
37	21.899846623062956	26.909719161539407	28.461338434144878	22.729095781252752
38	22.113274156867586	24.887721911746556	28.949345944325934	24.049657987059923
39	24.05704035400102	25.497924122489994	27.114993036334468	23.330042487174516
40	20.906532623450804	24.89785889321792	32.26171482467429	21.933893658656984
41	22.10963806568764	25.783522556987464	26.72350721929376	25.383332158031134
42	22.517541385328702	25.05222749149375	30.05449729386668	22.37573382931086
43	22.714331047370557	24.84320734093754	29.909935123318583	22.53252648837332
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	14.0
1	27.0
2	40.0
3	146.5
4	253.0
5	253.0
6	325.5
7	398.0
8	401.0
9	404.0
10	615.5
11	827.0
12	827.0
13	1217.0
14	1607.0
15	3883.5
16	6160.0
17	6774.0
18	7388.0
19	7388.0
20	7650.5
21	7913.0
22	9401.0
23	10889.0
24	13985.0
25	17081.0
26	17081.0
27	20206.0
28	23331.0
29	27421.5
30	31512.0
31	35354.0
32	39196.0
33	39196.0
34	42871.0
35	46546.0
36	49602.0
37	52658.0
38	54521.5
39	56385.0
40	56385.0
41	57850.0
42	59315.0
43	60488.0
44	61661.0
45	62463.0
46	63265.0
47	63265.0
48	64566.5
49	65868.0
50	79257.0
51	92646.0
52	73358.5
53	54071.0
54	54071.0
55	61693.0
56	69315.0
57	58241.0
58	47167.0
59	38153.5
60	29140.0
61	29140.0
62	25900.0
63	22660.0
64	19259.0
65	15858.0
66	13074.0
67	10290.0
68	10290.0
69	8382.0
70	6474.0
71	5425.0
72	4376.0
73	2935.0
74	1494.0
75	1494.0
76	1094.5
77	695.0
78	512.5
79	330.0
80	251.0
81	172.0
82	172.0
83	135.5
84	99.0
85	67.5
86	36.0
87	27.0
88	18.0
89	18.0
90	15.5
91	13.0
92	8.0
93	3.0
94	1.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	907568.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.821610894597775
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.2174923412093	39.16347198022897
2	11.835410374902287	11.556476002027301
3	3.2695843887117384	4.788791304382074
4	1.3615655287213335	2.6589528980292094
5	0.6825548247859136	1.6661713034964116
6	0.4158501690233512	1.2181485085506463
7	0.2858589525610902	0.9769266186872381
8	0.22280703080174752	0.8702238529906864
9	0.165006792525592	0.725030767768507
>10	1.2721552639838924	12.592360719153367
>50	0.1689469769591992	5.674747977894577
>100	0.09621734852289132	8.29282701620883
>500	0.0033879348060423717	1.1120691502198572
>1k	0.002032760883625423	2.253690559463901
>5k	4.517246408056495E-4	1.3718643825369603
>10k+	6.775869612084742E-4	5.0782469583614365
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGT	19192	2.1146624825908362	No Hit
ATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATG	14207	1.565392345256774	No Hit
TCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTT	12654	1.394275690636955	TruSeq Adapter, Index 8 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCC	7102	0.7825308957565714	No Hit
CTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTCTTC	5339	0.5882754790825592	RNA PCR Primer, Index 8 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3697	0.40735239673501056	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3651	0.40228390599933006	No Hit
CATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCGTC	3519	0.38773954127955146	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	2579	0.28416603494173437	No Hit
CACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTATGCCG	1627	0.17927031362939197	No Hit
TTATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTA	1492	0.16439539516598206	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1431	0.15767413571214497	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1318	0.14522327803536483	No Hit
TATACACATCTCCGAGCCCACGAGACGCTCATGAATCTCGTAT	1124	0.12384746928053875	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.509229060522186E-4	0.0
11	0.0	0.0	0.0	5.509229060522186E-4	0.0
12	0.0	0.0	0.0	7.712920684731061E-4	0.0
13	0.0	0.0	0.0	7.712920684731061E-4	0.0
14	0.0	0.0	0.0	7.712920684731061E-4	0.0
15	0.0	0.0	0.0	7.712920684731061E-4	0.0
16	0.0	0.0	0.0	8.814766496835499E-4	0.0
17	0.0	0.0	0.0	8.814766496835499E-4	0.0
18	0.0	0.0	0.0	8.814766496835499E-4	0.0
19	0.0	0.0	0.0	8.814766496835499E-4	0.0
20	0.0	0.0	0.0	0.0011018458121044373	0.0
21	0.0	0.0	0.0	0.001652768718156656	0.0
22	0.0	0.0	0.0	0.0022036916242088745	0.0
23	0.0	0.0	0.0	0.0027546145302610936	0.0
24	0.0	0.0	0.0	0.003195352855102868	0.0
25	0.0	0.0	0.0	0.003415722017523756	0.0
26	0.0	0.0	0.0	0.003746275761155087	0.0
27	0.0	0.0	0.0	0.004848121573259524	0.0
28	0.0	0.0	0.0	0.012561042257990585	0.0
29	0.0	0.0	0.0	0.028207252789873596	0.0
30	0.0	0.0	0.0	0.04859140031380569	0.0
31	0.0	0.0	0.0	0.09024117201135341	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2370	0.0	33.097046	1
GATTACG	80	0.0	32.375	1
TTATACA	2510	0.0	30.956175	2
CGTCTTC	685	0.0	30.78832	37
TATACAC	2605	0.0	30.324375	3
CCGTCTT	2005	0.0	24.728182	37
GGTATCA	650	0.0	24.192307	1
GCCGTCT	2085	0.0	23.779377	36
GTATCAA	1800	0.0	23.330555	1
AATGCCG	40	0.001930876	23.125	5
GCTTTAT	525	0.0	21.84762	1
CGAATGA	60	3.725174E-5	21.583334	10
GTTACGG	45	0.0038251972	20.555555	17
TGCCGTC	2445	0.0	20.278118	35
TTACGGG	120	2.382876E-10	20.041668	3
ATTGAGC	580	0.0	19.137932	6
ATGCCGT	2620	0.0	18.923664	34
ATACACA	4330	0.0	18.713627	4
TACACAT	4355	0.0	18.69116	5
AGCAACG	50	0.0070337695	18.5	2
>>END_MODULE