FastQCFastQC Report
Fri 10 Feb 2017
ERR1631192.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631192.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1514761
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGT146500.9671492730536367No Hit
ATACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATG102360.6757501678482613No Hit
TCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTT78370.5173753483222766TruSeq Adapter, Index 6 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCC57050.37662707186150157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35670.23548269330937355No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT33100.21851632039641897No Hit
CATCTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTC28040.18511171069231383No Hit
CTCCGAGCCCACGAGACCGATCAGTATCTCGTATGCCGTCTTC26250.17329466496694856RNA PCR Primer, Index 6 (95% over 22bp)
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC16700.11024841542659206No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA16220.10707959869576784No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCGCG303.6000137E-430.83333430
TTATACA20400.029.9264742
CTTATAC20550.029.8880791
TATACAC21350.029.1147543
GATTACG1300.027.0384621
CGTCTTC4950.026.16161737
GTATCAA19550.025.0767291
TTCGCGG451.3234836E-424.66666631
CCGTCTT13350.024.38951137
GCCGTCT13950.023.4731236
GGTATCA5900.021.9491521
CGCGGAA603.7272926E-521.58333433
TAGGACC1800.019.5277774
GGACCGT1153.0522642E-919.3043466
TGCCGTC17350.018.873235
TGGACCG701.21952195E-418.55
CTGACGT500.00703588818.54
GTACTAG701.21952195E-418.51
TCGCGGT609.238662E-418.517
ATACACA34550.018.4196834
Produced by FastQC (version 0.11.5)