FastQCFastQC Report
Fri 10 Feb 2017
ERR1631191.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631191.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227458
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT2449610.769460735608332No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG186088.18085097028902No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT140366.1708095560499086Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC96294.233309006497903No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC58892.589049406923476Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC46642.050488441822227No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26101.1474645868687845No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24921.095586877577399No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG20310.8929120980576635No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA18680.8212505165788848No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT15420.6779273536213279No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12620.554827704455328No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT10760.4730543660807709No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10040.4414001705809424No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8140.3578682657897282No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT6890.30291306526919254No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA6790.29851664922754967No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT5630.2475182231444926No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG5340.23476861662372833No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC5170.22729470935293547No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT4960.2180622356654855No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG4810.21146761160302122No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC4600.20223513791557124No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT4500.1978387218739284No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4380.19256302262395694No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG4010.1762962832698784No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG3840.16882237599908553No Hit
CTTTACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA3770.16574488476993554No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3650.1604691855199641No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG3620.15915026070747126No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT3570.15695205268664983No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG3380.14859886220752844No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3310.14552137097837842No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3230.14200423814506416No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG3120.137168180499257No Hit
CTGATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT3100.13628889729092844No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG3070.1349699724784356No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC3030.13321140606177845No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2980.131013198040957No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG2890.12705642360347844No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2880.12661678199931417No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2860.12573749879098559No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC2560.11254825066605705No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT2540.11166896745772846No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG2450.1077121930202499No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2440.1072725514160856No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA2430.10683290981192133No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA2410.10595362660359275No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2410.10595362660359275No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2330.10243649377027846No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2300.10111756895778562No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2290.10067792735362131No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAGAGT200.001838834337.03
GATTACG403.810237E-837.01
TTTACAC403.810237E-837.02
TGTATCC200.001838834337.03
TTACGGG403.810237E-837.03
GTTGTAT200.001838834337.01
CTTATAC28250.036.214161
CGTCTTC6800.034.2794137
TTATACA30300.033.7029722
ATTACGG451.0734948E-732.888892
TATACAC32050.031.978163
TATCAAT303.5904866E-430.8333321
TTGTATC250.00548719329.62
TTACACA502.7045826E-729.63
CGGGATC250.00548719329.616
CTCAGAG250.00548719329.62
CGTGCGG250.00548719329.610
GATTAGA250.00548719329.62
GTGCGGT250.00548719329.611
TAGATAC250.00548719329.65
Produced by FastQC (version 0.11.5)