##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631191.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227458
Sequences flagged as poor quality	0
Sequence length	43
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.127601579192643	31.0	31.0	34.0	28.0	34.0
2	31.228204767473557	31.0	31.0	34.0	28.0	34.0
3	30.694110561070616	31.0	31.0	34.0	26.0	34.0
4	34.80337029253752	37.0	35.0	37.0	32.0	37.0
5	35.020997283014886	35.0	35.0	37.0	32.0	37.0
6	35.21474733797009	36.0	35.0	37.0	32.0	37.0
7	35.15011122932585	37.0	35.0	37.0	32.0	37.0
8	35.21440881393488	37.0	35.0	37.0	32.0	37.0
9	37.06831151245505	39.0	37.0	39.0	33.0	39.0
10	36.852460674058506	39.0	37.0	39.0	33.0	39.0
11	36.910506555056315	39.0	37.0	39.0	33.0	39.0
12	36.751615682895306	39.0	37.0	39.0	32.0	39.0
13	36.898667006656176	39.0	37.0	39.0	33.0	39.0
14	37.6465897000765	39.0	37.0	41.0	32.0	41.0
15	37.700876645358704	39.0	37.0	41.0	32.0	41.0
16	37.68718620580503	39.0	37.0	41.0	32.0	41.0
17	37.577864924513534	39.0	37.0	41.0	32.0	41.0
18	37.77569485355538	39.0	37.0	41.0	32.0	41.0
19	37.712812914911765	39.0	37.0	41.0	32.0	41.0
20	37.86242295280887	39.0	37.0	41.0	33.0	41.0
21	37.7293917998048	39.0	37.0	41.0	32.0	41.0
22	37.50084411187999	39.0	36.0	41.0	32.0	41.0
23	37.59168286013242	39.0	37.0	41.0	32.0	41.0
24	37.40621125658363	39.0	36.0	41.0	31.0	41.0
25	37.40332720766032	39.0	36.0	41.0	32.0	41.0
26	37.097323461913845	39.0	36.0	40.0	31.0	41.0
27	36.956699698405856	39.0	35.0	40.0	31.0	41.0
28	37.09560885965761	39.0	36.0	40.0	31.0	41.0
29	36.95490156424483	39.0	35.0	40.0	31.0	41.0
30	36.887372613845194	39.0	35.0	40.0	31.0	41.0
31	36.756777075328195	39.0	35.0	40.0	30.0	41.0
32	36.44113638561844	38.0	35.0	40.0	30.0	41.0
33	36.183594333899	38.0	35.0	40.0	30.0	41.0
34	36.10374662575069	38.0	35.0	40.0	30.0	41.0
35	36.26442244282461	38.0	35.0	40.0	30.0	41.0
36	36.03005829647671	38.0	35.0	40.0	30.0	41.0
37	35.853463056916	38.0	35.0	40.0	29.0	41.0
38	35.93291948403661	38.0	35.0	40.0	29.0	41.0
39	35.63639880769197	38.0	34.0	40.0	27.0	41.0
40	35.28806636829656	38.0	34.0	40.0	26.0	41.0
41	35.281586051051185	38.0	34.0	40.0	26.0	41.0
42	35.10135497542404	38.0	34.0	40.0	26.0	41.0
43	33.95821206552419	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	2.0
8	4.0
9	7.0
10	19.0
11	21.0
12	18.0
13	19.0
14	13.0
15	24.0
16	10.0
17	17.0
18	21.0
19	32.0
20	68.0
21	105.0
22	198.0
23	360.0
24	624.0
25	1013.0
26	1567.0
27	2430.0
28	3430.0
29	4550.0
30	5902.0
31	7411.0
32	9263.0
33	11511.0
34	14480.0
35	18955.0
36	24413.0
37	34244.0
38	47938.0
39	38788.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.365491651205936	24.75665837209507	17.630067968592	30.247782008106995
2	9.920512797967097	18.784566821127417	46.97746397137054	24.317456409534945
3	11.547626374979117	31.38117806364252	40.300187287323375	16.771008274054992
4	7.444890924918007	24.23568307116039	24.11170413878606	44.20772186513554
5	11.839548400144203	36.94923898038319	32.730877788426874	18.48033483104573
6	23.259239068311512	38.6466952140615	14.66380606529557	23.43025965233142
7	18.664984304794732	35.139674137643	13.171662460761988	33.023679096800294
8	25.979301673275945	30.11852737648269	25.51987619692427	18.382294753317098
9	16.989070509720474	10.717143384712783	12.273474663454351	60.020311442112394
10	14.01533469915325	28.39952870420034	26.934642879124937	30.650493717521478
11	26.496759841377308	17.64633470794608	25.310606793342068	30.546298657334543
12	15.828416674726764	26.646237986793164	18.034098602818982	39.49124673566109
13	36.255044887407784	13.18221385926193	25.9010454677347	24.661695785595583
14	21.793034318423622	26.260232658336925	18.38273439492126	33.563998628318195
15	29.307828258403752	23.32034925129035	15.021234689481137	32.35058780082476
16	33.85899814471243	20.073156362932938	14.800534604190663	31.26731088816397
17	16.049116760017235	39.422662645411464	15.196651689542684	29.33156890502862
18	29.600629566777165	16.440837429327612	17.396178635176604	36.562354368718616
19	21.998786589172507	31.77729514899454	17.216365219073413	29.007553042759543
20	17.169763209031995	18.13961258781841	20.434102119951817	44.25652208319777
21	28.93325361165578	17.66743750494597	24.255906584951948	29.143402298446308
22	17.74657299369554	39.6068724775563	17.677109620237584	24.96944490851058
23	31.094092096123237	15.730376596998127	20.198893861723924	32.97663744515471
24	26.429494675940173	27.970438498535994	20.970464877032242	24.62960194849159
25	17.65205004880022	30.17568078502405	25.331709590341955	26.84055957583378
26	26.588644936647643	33.78997441285863	22.21640918323383	17.404971467259887
27	15.84336448926835	33.122598457737254	23.202964942978486	27.831072110015914
28	17.52719183321756	18.79819571085651	33.95000395677444	29.72460849915149
29	14.608411223170872	28.694967862198734	20.93837103992825	35.758249874702145
30	22.421282170774383	16.19463813099561	32.55062473071952	28.833454967510484
31	19.70077992420579	18.24204908158869	29.356628476465985	32.70054251773954
32	20.434981403160144	18.693121367461245	32.966086046654766	27.905811182723845
33	25.51899691371594	25.743653773443885	30.557289697438648	18.180059615401525
34	14.927591027794143	30.203378206086402	23.101847374020696	31.767183392098765
35	16.438199579702626	17.460805950988753	51.16329168461869	14.937702784689922
36	21.584644198049748	29.41158367698652	19.36533338022844	29.63843874473529
37	19.52360435772758	26.922332914208337	29.76461588513044	23.78944684293364
38	18.971854144501403	15.441971704666354	33.7350192123381	31.85115493849414
39	29.781322266088683	17.87187085088236	23.228464156020014	29.118342727008944
40	15.736531579456429	15.474065541770349	48.12580784144765	20.66359503732557
41	22.942257471709063	21.67872750134091	21.38768475938415	33.99133026756588
42	26.05711823721302	15.116636917584787	38.51392344960388	20.31232139559831
43	24.349110605034777	15.470548408937034	36.09061892745034	24.08972205857785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	0.0
1	2.5
2	5.0
3	41.5
4	78.0
5	78.0
6	125.0
7	172.0
8	212.5
9	253.0
10	354.5
11	456.0
12	456.0
13	681.0
14	906.0
15	2378.5
16	3851.0
17	3902.5
18	3954.0
19	3954.0
20	3246.0
21	2538.0
22	1953.0
23	1368.0
24	1332.0
25	1296.0
26	1296.0
27	1319.0
28	1342.0
29	1309.0
30	1276.0
31	1339.0
32	1402.0
33	1402.0
34	1706.0
35	2010.0
36	2024.0
37	2038.0
38	2277.5
39	2517.0
40	2517.0
41	3231.0
42	3945.0
43	5071.0
44	6197.0
45	8311.5
46	10426.0
47	10426.0
48	15158.0
49	19890.0
50	39973.5
51	60057.0
52	39488.0
53	18919.0
54	18919.0
55	29776.0
56	40633.0
57	32926.0
58	25219.0
59	16138.0
60	7057.0
61	7057.0
62	5698.5
63	4340.0
64	3386.0
65	2432.0
66	2016.5
67	1601.0
68	1601.0
69	1194.0
70	787.0
71	553.0
72	319.0
73	198.0
74	77.0
75	77.0
76	55.0
77	33.0
78	28.5
79	24.0
80	22.0
81	20.0
82	20.0
83	17.0
84	14.0
85	9.0
86	4.0
87	3.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	227458.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	17.935618883486182
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.17374252377684	12.944807393013216
2	10.846651632512991	3.8908281968539247
3	4.473477791940386	2.4070377827994616
4	2.6228061574664183	1.881666065823141
5	1.6398666535934896	1.4706011659295342
6	1.130012746347681	1.2160486771184131
7	0.8260613785665262	1.037114544223549
8	0.6520247083047358	0.9355573336615991
9	0.5392685557407589	0.8704903762452849
>10	4.2651240317678205	15.183022799813592
>50	0.5025002451220708	6.383156450861257
>100	0.2794391607020296	10.012397893237432
>500	0.01470732424747524	1.668879529407627
>1k	0.022060986371212864	8.154912115643327
>5k	0.0049024414158250805	6.822358413421378
>10k+	0.00735366212373762	25.12112126194726
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	24496	10.769460735608332	No Hit
ATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATG	18608	8.18085097028902	No Hit
TCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTT	14036	6.1708095560499086	Illumina PCR Primer Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCC	9629	4.233309006497903	No Hit
CTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCTTC	5889	2.589049406923476	Illumina PCR Primer Index 10 (95% over 22bp)
CATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTC	4664	2.050488441822227	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2610	1.1474645868687845	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2492	1.095586877577399	No Hit
CACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCG	2031	0.8929120980576635	No Hit
TTATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	1868	0.8212505165788848	No Hit
TATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTAT	1542	0.6779273536213279	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1262	0.554827704455328	No Hit
ACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGT	1076	0.4730543660807709	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1004	0.4414001705809424	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	814	0.3578682657897282	No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT	689	0.30291306526919254	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	679	0.29851664922754967	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	563	0.2475182231444926	No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG	534	0.23476861662372833	No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC	517	0.22729470935293547	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	496	0.2180622356654855	No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG	481	0.21146761160302122	No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC	460	0.20223513791557124	No Hit
ATCTCCGAGCCCACGAGACACTCGCTAATCTCGTATGCCGTCT	450	0.1978387218739284	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	438	0.19256302262395694	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	401	0.1762962832698784	No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG	384	0.16882237599908553	No Hit
CTTTACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGTA	377	0.16574488476993554	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	365	0.1604691855199641	No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG	362	0.15915026070747126	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	357	0.15695205268664983	No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG	338	0.14859886220752844	No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG	331	0.14552137097837842	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	323	0.14200423814506416	No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG	312	0.137168180499257	No Hit
CTGATACACATCTCCGAGCCCACGAGACACTCGCTAATCTCGT	310	0.13628889729092844	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	307	0.1349699724784356	No Hit
GAGATGTGTATAAGAGACAGACACATCTCCGAGCCCACGAGAC	303	0.13321140606177845	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	298	0.131013198040957	No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG	289	0.12705642360347844	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	288	0.12661678199931417	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	286	0.12573749879098559	No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC	256	0.11254825066605705	No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT	254	0.11166896745772846	No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG	245	0.1077121930202499	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	244	0.1072725514160856	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	243	0.10683290981192133	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	241	0.10595362660359275	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	241	0.10595362660359275	No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA	233	0.10243649377027846	No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG	230	0.10111756895778562	No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA	229	0.10067792735362131	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	8.792832083285706E-4	0.0
17	0.0	0.0	0.0	0.0013189248124928557	0.0
18	0.0	0.0	0.0	0.0021982080208214263	0.0
19	0.0	0.0	0.0	0.0021982080208214263	0.0
20	0.0	0.0	0.0	0.0021982080208214263	0.0
21	0.0	0.0	0.0	0.0026378496249857115	0.0
22	0.0	0.0	0.0	0.0035171328333142822	0.0
23	0.0	0.0	0.0	0.003956774437478568	0.0
24	0.0	0.0	0.0	0.004396416041642853	0.0
25	0.0	0.0	0.0	0.004836057645807138	0.0
26	0.0	0.0	0.0	0.005275699249971423	0.0
27	0.0	0.0	0.0	0.006594624062464279	0.0
28	0.0	0.0	0.0	0.016266739354078553	0.0
29	0.0	0.0	0.0	0.04000738597894996	0.0
30	0.0	0.0	0.0	0.0808940551662285	0.0
31	0.0	0.0	0.0	0.1490385038116927	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAGAGT	20	0.0018388343	37.0	3
GATTACG	40	3.810237E-8	37.0	1
TTTACAC	40	3.810237E-8	37.0	2
TGTATCC	20	0.0018388343	37.0	3
TTACGGG	40	3.810237E-8	37.0	3
GTTGTAT	20	0.0018388343	37.0	1
CTTATAC	2825	0.0	36.21416	1
CGTCTTC	680	0.0	34.27941	37
TTATACA	3030	0.0	33.702972	2
ATTACGG	45	1.0734948E-7	32.88889	2
TATACAC	3205	0.0	31.97816	3
TATCAAT	30	3.5904866E-4	30.833332	1
TTGTATC	25	0.005487193	29.6	2
TTACACA	50	2.7045826E-7	29.6	3
CGGGATC	25	0.005487193	29.6	16
CTCAGAG	25	0.005487193	29.6	2
CGTGCGG	25	0.005487193	29.6	10
GATTAGA	25	0.005487193	29.6	2
GTGCGGT	25	0.005487193	29.6	11
TAGATAC	25	0.005487193	29.6	5
>>END_MODULE