FastQCFastQC Report
Fri 10 Feb 2017
ERR1631190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences342858
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATG167764.892987767530581No Hit
TCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTT144414.211947803463825TruSeq Adapter, Index 3 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGT137143.9999066668999994No Hit
ACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCC83122.424327272515152No Hit
CTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTC64341.8765786418867287RNA PCR Primer, Index 3 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT53271.5537044490722107No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT47611.388621528446179No Hit
CATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTC43231.2608718478203804No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA25970.7574564396922341No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT19990.5830402090661441No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA19220.5605819318785036No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA18840.5494986262534344No Hit
CACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCG18670.5445403053159035No Hit
TTATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTA17870.5212070303157575No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT17580.5127487181282047No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14620.4264156006276651No Hit
TATACACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTAT14390.41970728406512314No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11850.3456241359396601No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11730.3421241446896383No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11480.33483249625209266No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC11360.33133250500207084No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10810.31529087843947057No Hit
ACATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGT9750.2843742890642773No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG9120.2659993350016625No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8790.25637435906410233No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8160.2379994050014875No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG8070.2353744115639711No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7590.22137444656388358No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG7430.2167077915638544No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7320.21349946625133434No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG7290.2126244684388289No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG7110.2073744815637961No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6850.19979116718874868No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6810.1986245034387414No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG6810.1986245034387414No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6620.19308285062620678No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA6570.19162452093869764No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6520.19016619125118853No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6230.18170787906363567No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG6120.17849955375111562No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6010.17529122843859557No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5900.17208290312607552No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5890.1717912371885737No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5470.15954126781349715No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5420.158082938125988No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5420.158082938125988No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5310.15487461281346795No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG5290.15429128093846434No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC5210.15195795343844976No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5100.1487496281259297No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4920.14349964125089687No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4900.14291630937589322No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4860.14174964562588593No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4860.14174964562588593No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4840.1411663137508823No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4780.13941631812587135No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4780.13941631812587135No Hit
ATCTCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCT4720.13766632250086042No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4630.135041329063344No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA4620.1347496631258422No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4490.1309580059383185No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4440.12949967625080935No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC4390.12804134656330027No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4320.1259996850007875No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4100.11958303437574741No Hit
CATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAACT4080.11899970250074375No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4070.11870803656324191No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC4060.1184163706257401No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4030.11754137281323462No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4000.11666637500072916No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3970.11579137718822369No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3930.1146247134382164No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3880.11316638375070728No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3860.11258305187570364No Hit
TCCGAGCCCACGAGACGTAGAGGAATCTCGTATGCCGTCTTCT3800.11083305625069272TruSeq Adapter, Index 3 (95% over 23bp)
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3780.11024972437568906No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3750.10937472656318359No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC3720.10849972875067812No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA3690.10762473093817267No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3670.10704139906316901No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3650.10645806718816538No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3620.10558306937565991No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA3600.10499973750065625No Hit
CACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTA3550.10354140781314712No Hit
CCTATGGATTCAGTTAATGATAGTGTGTCGAAACACACTGGGT3550.10354140781314712No Hit
TATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATA3510.10237474406313983No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAACG403.8198777E-837.02
TAGCAAC403.8198777E-837.01
ATTATAG200.001840135337.01
CTTATAC15500.036.1645161
GATACAC700.034.3571433
CGTCTTC7350.034.23129337
TGATACA750.032.0666662
TTATACA17650.031.7592072
TCGCCGA352.3834573E-531.7142871
GACAGAC352.3834573E-531.71428716
TCAGAGT352.3834573E-531.7142873
TGTCAAC303.5942582E-430.8333322
TTAGGAC601.2641976E-930.8333323
GTGTCAA303.5942582E-430.8333321
TAGGACC601.2641976E-930.8333324
TTTACAC502.7113674E-729.62
AGCCCGC250.00549103129.618
GACCGTT751.4551915E-1129.67
CTATACA250.00549103129.61
TTACTTT250.00549103129.63
Produced by FastQC (version 0.11.5)