FastQCFastQC Report
Fri 10 Feb 2017
ERR1631189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132380
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGT176381.5576043377664741No Hit
ATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATG137531.214521627015666No Hit
TCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTT106380.9394372913686219RNA PCR Primer, Index 41 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCC76270.6735371518394885No Hit
CTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTCTTC39240.34652678429502465RNA PCR Primer, Index 41 (95% over 23bp)
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38630.34113990003355765No Hit
CATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCGTC37850.33425175294512444No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT36210.31976898214380334No Hit
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC25280.2232466133276815No Hit
CACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTATGCCG18340.1619597661562373No Hit
TTATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTA13720.12116074109397906No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13630.12036595489146754No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA12320.10879740016602202No Hit
TATACACATCTCCGAGCCCACGAGACCCTAAGACATCTCGTAT11600.10243911054592982No Hit
GTTCAAGGGCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCG11580.10226249138981613No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC23300.031.5214581
CGCTAGT250.005496309629.612
TTATACA25500.028.7294122
TATACAC25700.028.6498073
GGTATCA6050.027.5206621
CGTCTTC5400.026.37962737
CCGTCTT17600.026.17329637
GCCGTCT18250.025.34246436
GTATCAA18550.025.0323451
TTAACGG802.7257556E-823.12500235
ACCCGTT502.702068E-422.210
GATTACG603.726224E-521.5833321
TGCCGTC22800.020.28508835
TAACCGT656.902013E-519.9230777
TACCGTT759.265572E-619.7333349
GCGTCAA851.2452747E-619.58823626
ATGCCGT24300.019.03292334
GACCGTT1002.8748218E-718.57
TCTAGAC701.2191768E-418.53
ACCGTTT801.6166357E-518.510
Produced by FastQC (version 0.11.5)