FastQCFastQC Report
Fri 10 Feb 2017
ERR1631188.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631188.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753169
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGT148411.970474090144443No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATG109571.4547863759660846No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTT91481.2146012382347122RNA PCR Primer, Index 33 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCC54390.7221486811060998No Hit
CTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC38970.5174137544163394RNA PCR Primer, Index 33 (95% over 23bp)
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC26990.3583525078700796No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT24320.32290229682846744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT23480.3117494214445895No Hit
CACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCG12170.16158392074023228No Hit
TTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTA11050.14671342022839495No Hit
GAATACACCTCTTAAATTTACAGGACTTAACATTTCAAACATC10600.14073866555846032No Hit
ATTGAAAGCTGAGTATTTTTAAGACAAAGGTTTCAGGAAGAAA9670.1283908392405954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT9520.1263992543506172No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA9390.12467321411263607No Hit
TATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTAT8880.11790182548671016No Hit
GTGATATAGTTATTTCCTGTCAGTGATTTTGGTCTGAATCAAC8750.11617578524872904No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT8630.11458251733674647No Hit
GTTTATAAAGTCCCTGGCGGCAAGATTATCAAGAATGGTGTTC8230.10927162429680458No Hit
ATCTTGAATAGTGATATAGTTATTTCCTGTCAGTGATTTTGGT7710.1023674633448801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC17800.033.1544951
CGTCTTC4950.031.76767737
TTAGTAC303.5983103E-430.8333343
TTATACA19600.030.4872472
GATTACG801.8189894E-1230.06251
TATACAC20750.029.6891573
GCAACGC250.00549515529.63
GGTATCA4050.025.5802461
CCGTCTT14950.024.62541837
GCCGTCT15100.024.2582836
GTATCAA13100.023.3015251
CGCATCA400.001930572723.1259
ATTACGG1107.4578566E-1121.8636362
GGCGGTT705.098069E-621.14285927
CTAGACC806.95356E-720.81253
GTCGCCA1450.020.41379412
TAAACCG656.898092E-519.92307714
TGCCGTC18400.019.9076135
TCGCCAT1500.019.73333213
ATACACA32100.019.3644874
Produced by FastQC (version 0.11.5)