FastQCFastQC Report
Fri 10 Feb 2017
ERR1631187.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631187.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1009349
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT167901.663448420714738No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG124991.2383229190299887No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT87080.8627342970568158TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC67020.663992335653971No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29150.2888000087184908No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC28890.28622409097348883No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC28740.28473798458214156Illumina Paired End PCR Primer 2 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT27190.2693815518715528No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG15150.15009674552607669No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA13260.13137180499510082No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA11440.11334038078008697No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC21300.032.309861
ACGGTAA352.3880226E-531.71428522
TTATACA22800.030.184212
TATACAC23350.030.0278363
CGGTAAC405.9394224E-527.75000223
CGTCTTC3800.027.26315937
GTATCAA18450.026.3712731
CCGTCTT13400.026.36940437
GATTACG856.91216E-1126.1176471
GCCGTCT13550.025.80442636
GGTATCA5150.025.1456321
TGCCGTC17150.020.38775435
GTCGAAC555.1424967E-420.18181817
TCGAACG555.1424967E-420.18181818
GAACGGT656.901066E-519.92307720
TTACGGG1052.2586391E-819.3809533
ATGCCGT18100.019.31767834
ATTACGG1153.0486262E-919.3043482
GCGGTAT500.007034303618.4999986
TAGCACG500.007034303618.4999985
Produced by FastQC (version 0.11.5)