##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631187.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1009349
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.469778045056763	31.0	31.0	34.0	28.0	34.0
2	31.463100473671645	31.0	31.0	34.0	28.0	34.0
3	30.93187688302064	31.0	31.0	34.0	27.0	34.0
4	35.09292425117576	37.0	35.0	37.0	32.0	37.0
5	35.29425699138752	37.0	35.0	37.0	33.0	37.0
6	35.479322811039594	37.0	35.0	37.0	33.0	37.0
7	35.48684746306778	37.0	35.0	37.0	33.0	37.0
8	35.504959137027924	37.0	35.0	37.0	33.0	37.0
9	37.2832677299923	39.0	37.0	39.0	34.0	39.0
10	37.11290643771381	39.0	37.0	39.0	33.0	39.0
11	37.17229818427521	39.0	37.0	39.0	33.0	39.0
12	37.10985001223561	39.0	37.0	39.0	33.0	39.0
13	37.16981341438888	39.0	37.0	39.0	33.0	39.0
14	38.29687452011148	40.0	38.0	41.0	34.0	41.0
15	38.30834924292787	40.0	38.0	41.0	34.0	41.0
16	38.30684034957185	40.0	38.0	41.0	33.0	41.0
17	38.290809224559595	40.0	38.0	41.0	33.0	41.0
18	38.31987152114878	40.0	38.0	41.0	33.0	41.0
19	38.35127790288592	40.0	38.0	41.0	34.0	41.0
20	38.36504420175777	40.0	38.0	41.0	34.0	41.0
21	38.30054322142292	40.0	38.0	41.0	34.0	41.0
22	38.2459476355552	40.0	38.0	41.0	34.0	41.0
23	38.23528630830367	40.0	38.0	41.0	34.0	41.0
24	38.18424548892405	40.0	38.0	41.0	33.0	41.0
25	38.16638546231284	40.0	38.0	41.0	33.0	41.0
26	38.061686294829634	40.0	37.0	41.0	33.0	41.0
27	37.98584434125362	40.0	37.0	41.0	33.0	41.0
28	37.979048872094786	40.0	37.0	41.0	33.0	41.0
29	37.9069628047385	40.0	37.0	41.0	33.0	41.0
30	37.833687852269136	40.0	37.0	41.0	33.0	41.0
31	37.83719902630309	40.0	37.0	41.0	33.0	41.0
32	37.70863992533801	40.0	37.0	41.0	33.0	41.0
33	37.703608959834504	40.0	37.0	41.0	33.0	41.0
34	37.62655038049277	40.0	37.0	41.0	32.0	41.0
35	37.579595362951764	40.0	37.0	41.0	32.0	41.0
36	37.583311619667725	40.0	37.0	41.0	32.0	41.0
37	37.49041312766942	40.0	37.0	41.0	32.0	41.0
38	37.46882396475352	40.0	37.0	41.0	32.0	41.0
39	37.40564561910697	39.0	37.0	41.0	32.0	41.0
40	37.33939895913108	39.0	36.0	41.0	31.0	41.0
41	37.29121839918601	39.0	36.0	41.0	31.0	41.0
42	37.2399180065567	39.0	36.0	41.0	31.0	41.0
43	36.58605199985337	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	6.0
10	12.0
11	11.0
12	8.0
13	8.0
14	5.0
15	9.0
16	8.0
17	14.0
18	14.0
19	46.0
20	70.0
21	169.0
22	360.0
23	685.0
24	1263.0
25	2121.0
26	3534.0
27	5508.0
28	8053.0
29	11765.0
30	16423.0
31	21888.0
32	28828.0
33	37607.0
34	49922.0
35	67216.0
36	93905.0
37	138698.0
38	243974.0
39	277217.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.66759663902178	21.94959325268069	14.838772317602732	24.544037790694794
2	13.760354446281712	22.338854053454256	41.00652995148358	22.894261548780452
3	14.76357533420056	28.07254973255039	34.63430389290523	22.529571040343825
4	11.884095590326043	19.296794270366348	37.9385128434268	30.88059729588081
5	12.482104802204192	38.52562394176841	35.67150708030622	13.320764175721184
6	27.615027111534268	40.193431607897764	17.79622311014327	14.395318170424702
7	24.411576174346038	33.52348890225284	22.673426139026244	19.391508784374878
8	24.603382972589262	35.66318488451467	22.08482893429329	17.648603208602772
9	24.312799636201156	15.242398813492658	19.599860900441772	40.84494064986442
10	14.385509868241808	29.23963861855513	33.62335525175138	22.75149626145169
11	31.451163076398746	23.140459840946985	25.62176214569985	19.786614936954415
12	20.769228482913242	26.249988854202066	29.68745201114778	23.293330651736913
13	28.590408273055207	21.118364411120435	27.273222641524384	23.01800467429997
14	21.32097024914078	22.31824671149424	27.10965186471676	29.251131174648215
15	23.595604691736952	28.723464331960503	23.44095055327741	24.239980423025138
16	24.080372596594437	28.007458272609377	23.78503371975402	24.12713541104217
17	21.165523520605856	30.18549579976797	25.4743403916782	23.174640287947977
18	23.542005787889025	26.561476753828455	26.286249850150938	23.610267608131576
19	23.77998095802344	28.079286748191162	26.185590910577016	21.955141383208385
20	22.98154553083225	25.939491692169902	26.358870915808108	24.720091861189736
21	23.83784003352656	26.440804914851057	26.62894598399562	23.092409067626757
22	22.024592088564017	30.37928407319966	25.971393442704162	21.62473039553217
23	22.719891732195702	27.03940856928575	26.610716412261766	23.62998328625679
24	24.53234708708286	27.81842553963	26.006168332261687	21.643059041025452
25	22.291992165247105	27.767996996083617	26.141404013874293	23.79860682479499
26	24.703744690884914	27.456013727660107	26.494106597420714	21.34613498403426
27	22.190837856876065	28.695624605562596	26.105737460481954	23.007800077079384
28	21.331372993880212	26.926761704821622	28.383938558417356	23.357926742880807
29	23.63889992460487	28.104352409325216	26.02637937918401	22.230368286885906
30	21.43807543277895	27.56172542896461	27.21992095895473	23.78027817930171
31	24.529176726781323	25.620176965549078	27.69587129922356	22.154775008446038
32	23.65930911904604	26.605069207974648	26.93855148219298	22.79707019078634
33	22.63716514307737	27.612253046270418	28.104253335565794	21.646328475086417
34	23.289565848878834	27.778003445785355	26.389980076266976	22.542450629068835
35	21.63136833741352	26.22621115194051	29.145320399584286	22.997100111061684
36	24.42544650066528	26.366598669043118	25.706073914968957	23.501880915322648
37	22.66797708225797	27.02415121033458	27.570939288590964	22.736932418816487
38	22.380365958652558	25.484544988898783	28.37462562503158	23.76046342741708
39	23.616509254975238	26.04599598354979	26.910414534516804	23.427080226958168
40	21.519018694227665	25.418760012641812	30.814118803307878	22.24810248982265
41	22.460813851304156	26.268218425935924	26.69750502551645	24.57346269724347
42	23.049609203555953	25.425695175801433	28.963321903523955	22.561373717118656
43	22.883165287725056	25.08824995120617	29.038617960685553	22.98996680038322
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	16.0
1	23.5
2	31.0
3	92.0
4	153.0
5	153.0
6	200.5
7	248.0
8	271.5
9	295.0
10	485.0
11	675.0
12	675.0
13	1042.5
14	1410.0
15	3219.5
16	5029.0
17	5756.5
18	6484.0
19	6484.0
20	7027.5
21	7571.0
22	9419.5
23	11268.0
24	14533.0
25	17798.0
26	17798.0
27	22100.0
28	26402.0
29	31783.0
30	37164.0
31	40734.5
32	44305.0
33	44305.0
34	48508.0
35	52711.0
36	55920.0
37	59129.0
38	62540.0
39	65951.0
40	65951.0
41	68324.5
42	70698.0
43	68891.5
44	67085.0
45	68430.5
46	69776.0
47	69776.0
48	69589.5
49	69403.0
50	70387.5
51	71372.0
52	85253.5
53	99135.0
54	99135.0
55	78097.0
56	57059.0
57	59044.0
58	61029.0
59	50364.5
60	39700.0
61	39700.0
62	32001.5
63	24303.0
64	20304.0
65	16305.0
66	13755.5
67	11206.0
68	11206.0
69	8963.0
70	6720.0
71	5527.0
72	4334.0
73	3289.0
74	2244.0
75	2244.0
76	1729.0
77	1214.0
78	905.0
79	596.0
80	446.5
81	297.0
82	297.0
83	213.0
84	129.0
85	95.0
86	61.0
87	46.0
88	31.0
89	31.0
90	20.0
91	9.0
92	5.0
93	1.0
94	1.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1009349.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.35732061709899
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.05051770655326	41.3887330050967
2	12.652323983313918	13.248835666915554
3	3.6090524731370985	5.668809523799193
4	1.4662691327244592	3.0707969237204074
5	0.7622356661532358	1.9954308579286497
6	0.46940115681514083	1.4745952119244492
7	0.308662553872865	1.131252100692993
8	0.2182700941677152	0.9142429841170762
9	0.17193639744384198	0.8101916178044575
>10	1.0837015031653245	11.018785925104671
>50	0.12287568264998033	4.501438540721937
>100	0.0788758801736656	7.4350025851124215
>500	0.003792109567358791	1.37750310385474
>1k	0.001327238348575577	1.5270072602690699
>5k	3.7921095673587916E-4	1.529786886019137
>10k+	3.7921095673587916E-4	2.9075878069185275
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGT	16790	1.663448420714738	No Hit
ATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATG	12499	1.2383229190299887	No Hit
TCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTT	8708	0.8627342970568158	TruSeq Adapter, Index 5 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCC	6702	0.663992335653971	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2915	0.2888000087184908	No Hit
CATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTC	2889	0.28622409097348883	No Hit
CTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCGTCTTC	2874	0.28473798458214156	Illumina Paired End PCR Primer 2 (95% over 23bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2719	0.2693815518715528	No Hit
CACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTATGCCG	1515	0.15009674552607669	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1326	0.13137180499510082	No Hit
TTATACACATCTCCGAGCCCACGAGACACTGAGCGATCTCGTA	1144	0.11334038078008697	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	9.907375942315295E-5	0.0
3	0.0	0.0	0.0	9.907375942315295E-5	0.0
4	0.0	0.0	0.0	1.981475188463059E-4	0.0
5	0.0	0.0	0.0	1.981475188463059E-4	0.0
6	0.0	0.0	0.0	1.981475188463059E-4	0.0
7	0.0	0.0	0.0	2.972212782694588E-4	0.0
8	0.0	0.0	0.0	2.972212782694588E-4	0.0
9	0.0	0.0	0.0	2.972212782694588E-4	0.0
10	0.0	0.0	0.0	2.972212782694588E-4	0.0
11	0.0	0.0	0.0	2.972212782694588E-4	0.0
12	0.0	0.0	0.0	2.972212782694588E-4	0.0
13	0.0	0.0	0.0	2.972212782694588E-4	0.0
14	0.0	0.0	0.0	3.962950376926118E-4	0.0
15	0.0	0.0	0.0	3.962950376926118E-4	0.0
16	0.0	0.0	0.0	3.962950376926118E-4	0.0
17	0.0	0.0	0.0	4.953687971157647E-4	0.0
18	0.0	0.0	0.0	5.944425565389176E-4	0.0
19	0.0	0.0	0.0	6.935163159620706E-4	0.0
20	0.0	0.0	0.0	7.925900753852236E-4	0.0
21	0.0	0.0	0.0	8.916638348083765E-4	0.0
22	0.0	0.0	0.0	9.907375942315294E-4	0.0
23	0.0	0.0	0.0	0.0010898113536546824	0.0
24	0.0	0.0	0.0	0.001486106391347294	0.0
25	0.0	0.0	0.0	0.0016842539101936	0.0
26	0.0	0.0	0.0	0.0024768439855788237	0.0
27	0.0	0.0	0.0	0.0028731390232714354	0.0
28	0.0	0.0	0.0	0.0101055234611616	0.0
29	0.0	0.0	0.0	0.02625454624713553	0.0
30	9.907375942315295E-5	0.0	0.0	0.050428543546384846	0.0
31	9.907375942315295E-5	0.0	0.0	0.10680151265815888	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2130	0.0	32.30986	1
ACGGTAA	35	2.3880226E-5	31.714285	22
TTATACA	2280	0.0	30.18421	2
TATACAC	2335	0.0	30.027836	3
CGGTAAC	40	5.9394224E-5	27.750002	23
CGTCTTC	380	0.0	27.263159	37
GTATCAA	1845	0.0	26.371273	1
CCGTCTT	1340	0.0	26.369404	37
GATTACG	85	6.91216E-11	26.117647	1
GCCGTCT	1355	0.0	25.804426	36
GGTATCA	515	0.0	25.145632	1
TGCCGTC	1715	0.0	20.387754	35
GTCGAAC	55	5.1424967E-4	20.181818	17
TCGAACG	55	5.1424967E-4	20.181818	18
GAACGGT	65	6.901066E-5	19.923077	20
TTACGGG	105	2.2586391E-8	19.380953	3
ATGCCGT	1810	0.0	19.317678	34
ATTACGG	115	3.0486262E-9	19.304348	2
GCGGTAT	50	0.0070343036	18.499998	6
TAGCACG	50	0.0070343036	18.499998	5
>>END_MODULE