FastQCFastQC Report
Fri 10 Feb 2017
ERR1631186.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631186.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences209221
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATG102274.888132644428619No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGT94184.501460178471568No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTT83954.012503524980763TruSeq Adapter, Index 10 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCC49342.3582718751941725No Hit
CTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC43392.0738835967708784RNA PCR Primer, Index 10 (95% over 22bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT34621.6547096132797379No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT29221.3966093269796052No Hit
CATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC27221.3010166283499265No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA17210.8225751717083849No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT12360.5907628775314142No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA11840.5659087758876977No Hit
CACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCG10520.5028175947921097No Hit
TTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTA10520.5028175947921097No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA10320.4932583249291419No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT9730.4650584788333867No Hit
TATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTAT8710.4163062025322506No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT8710.4163062025322506No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT7790.3723335611625984No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG7450.356082802395553No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC7280.34795742301203036No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC7120.34031000712165604No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG6440.30780848958756535No Hit
ACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGT5620.26861548314939704No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG5440.260012140272726No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG5020.23993767356049345No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG4930.23563600212215793No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC4850.23181229417697075No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT4620.22081913383455773No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG4550.21747338938251895No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4520.21603949890307378No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG4250.20313448458806715No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4230.2021785576017704No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4220.20170059410862198No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG4190.20026670362917678No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4020.1921413242456541No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT4020.1921413242456541No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG4000.19118539725935732No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3980.19022947027306053No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3780.18067020041009269No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG3730.1782803829443507No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC3620.1730227845197184No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3550.1696770400676796No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3490.16680925910878927No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3380.16155166068415694No Hit
ATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCT3240.15486017178007944No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA3210.15342628130063427No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC3120.14912460986229872No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3070.14673479239655676No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC3020.1443449749308148No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG2990.1429110844513696No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA2980.14243312095822122No Hit
TCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCT2980.14243312095822122TruSeq Adapter, Index 10 (95% over 23bp)
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG2960.14147719397192443No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT2920.13956533999933085No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2880.13765348602673727No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG2790.13335181458840176No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG2780.13287385109525335No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG2770.13239588760210497No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA2690.1285721796569178No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC2660.12713828917747264No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG2640.12618236219117585No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG2620.12522643520487906No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC2620.12522643520487906No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC2570.12283661773913708No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2540.12140272725969191No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT2480.11853494630080154No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA2460.11757901931450476No Hit
TAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAGTA2380.11375531136931762No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2280.10897567643783368No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG2280.10897567643783368No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC2270.10849771294468527No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA2220.10610789547894331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC2170.10371807801320135No Hit
CAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACAC2170.10371807801320135No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA2170.10371807801320135No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA2150.10276215102690456No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2150.10276215102690456No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA2140.10228418753375618No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGAA200.001838496537.017
CTATACA308.2794995E-637.01
TTACTTT200.001838496537.03
ACAGACA308.2794995E-637.017
GACAGAC308.2794995E-637.016
TCGGAGA200.001838496537.01
TAGCAAC355.601996E-737.01
ACTCAGA403.8076905E-837.01
CAGACAC308.2794995E-637.018
GATTACG200.001838496537.01
AGCAACG355.601996E-737.02
TTACGGG251.230177E-436.9999963
CTTATAC11500.034.4260861
CGTCTTC5350.032.50467337
GACCGTT705.456968E-1231.7142877
TTATACA12650.031.2964442
CGTTATT551.8404535E-830.27272810
TATACAC13450.029.8475843
TAGGACC751.4551915E-1129.64
GGGCTTC751.4551915E-1129.637
Produced by FastQC (version 0.11.5)