FastQCFastQC Report
Fri 10 Feb 2017
ERR1631185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences979903
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT170041.735273797508529No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG125081.2764528733966525No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT78790.8040591772859149TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC68540.6994569870691283No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC33100.33778853621225774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT28110.2868651284872074No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT25560.2608421445796166No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC24410.24910628909187948RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG17010.1735886103012237No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA14960.15266817225786633No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA11520.11756265671193984No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT10210.10419398654764808No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT10020.10225501911923936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC22750.032.771431
TTATACA24100.031.6265562
TATACAC24450.031.1738243
CCGTCTT11200.025.60267837
GGTATCA5900.025.3983061
GCCGTCT11350.025.26431736
CGTCTTC3850.024.98701337
GATTACG1051.8189894E-1224.6666661
GTATCAA15150.022.7128721
TATCCCG705.1006573E-621.14285710
CCACGTT1151.3460522E-1020.91304437
TTACGGG1153.0486262E-919.3043483
ATACACA41150.018.9270974
TACACAT41300.018.5447945
AGGACCG609.2354877E-418.55
TATACAG1800.018.55
CGATCAT500.00703415918.529
TGCCGTC15850.018.3249235
CCCGTAT752.0668747E-417.26666613
TATCGTG650.001579709717.07692137
Produced by FastQC (version 0.11.5)