##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631185.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	979903
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.47798608637794	31.0	31.0	34.0	28.0	34.0
2	31.463334636183376	31.0	31.0	34.0	28.0	34.0
3	30.94122887673576	31.0	31.0	34.0	27.0	34.0
4	35.0957094732846	37.0	35.0	37.0	32.0	37.0
5	35.2947016184255	37.0	35.0	37.0	33.0	37.0
6	35.488595299738854	37.0	35.0	37.0	33.0	37.0
7	35.48853100766096	37.0	35.0	37.0	33.0	37.0
8	35.506908336845584	37.0	35.0	37.0	33.0	37.0
9	37.28526292908584	39.0	37.0	39.0	34.0	39.0
10	37.11280912498482	39.0	37.0	39.0	33.0	39.0
11	37.17283751555001	39.0	37.0	39.0	33.0	39.0
12	37.10506448087209	39.0	37.0	39.0	33.0	39.0
13	37.175880673903436	39.0	37.0	39.0	33.0	39.0
14	38.308452979529605	40.0	38.0	41.0	34.0	41.0
15	38.31000109194481	40.0	38.0	41.0	34.0	41.0
16	38.31018988614179	40.0	38.0	41.0	33.0	41.0
17	38.280871678115076	40.0	38.0	41.0	33.0	41.0
18	38.319978610127734	40.0	38.0	41.0	33.0	41.0
19	38.35464122469265	40.0	38.0	41.0	34.0	41.0
20	38.344772900991224	40.0	38.0	41.0	34.0	41.0
21	38.31074096109513	40.0	38.0	41.0	34.0	41.0
22	38.23387110765045	40.0	38.0	41.0	34.0	41.0
23	38.23754085863601	40.0	38.0	41.0	34.0	41.0
24	38.196598030621395	40.0	38.0	41.0	33.0	41.0
25	38.168837119592446	40.0	38.0	41.0	33.0	41.0
26	38.07617080466128	40.0	37.0	41.0	33.0	41.0
27	37.97984596434545	40.0	37.0	41.0	33.0	41.0
28	37.98757325980225	40.0	37.0	41.0	33.0	41.0
29	37.909263467914684	40.0	37.0	41.0	33.0	41.0
30	37.820324052482746	40.0	37.0	41.0	33.0	41.0
31	37.80979239781897	40.0	37.0	41.0	33.0	41.0
32	37.70991720609081	40.0	37.0	41.0	33.0	41.0
33	37.69669344822906	40.0	37.0	41.0	33.0	41.0
34	37.64075117639195	40.0	37.0	41.0	32.0	41.0
35	37.617271301343095	40.0	37.0	41.0	32.0	41.0
36	37.58208516557251	40.0	37.0	41.0	32.0	41.0
37	37.50290079732382	40.0	37.0	41.0	32.0	41.0
38	37.47613386222922	40.0	37.0	41.0	32.0	41.0
39	37.398941527885924	39.0	37.0	41.0	32.0	41.0
40	37.32367081231509	39.0	36.0	41.0	31.0	41.0
41	37.26259333832022	39.0	36.0	41.0	31.0	41.0
42	37.18974020897987	39.0	36.0	41.0	31.0	41.0
43	36.54375177951287	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	7.0
10	11.0
11	11.0
12	12.0
13	6.0
14	2.0
15	9.0
16	10.0
17	8.0
18	16.0
19	36.0
20	75.0
21	163.0
22	322.0
23	674.0
24	1209.0
25	2109.0
26	3358.0
27	5300.0
28	8004.0
29	11401.0
30	15840.0
31	21664.0
32	28208.0
33	36694.0
34	48510.0
35	65282.0
36	91008.0
37	134773.0
38	232639.0
39	272540.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.3613480109766	22.05014169769865	14.683800335339313	24.904709955985442
2	13.922908696064814	21.85695931127877	41.04702200115726	23.173109991499157
3	14.383158333018676	28.57333838145204	33.819980140891495	23.223523144637788
4	11.677788515802074	19.46784528672736	38.281238040908136	30.573128156562433
5	12.61206466354323	37.99498521792463	35.98141856898081	13.411531549551334
6	27.61640693007369	40.18060971341041	17.827682944128142	14.375300412387757
7	24.814394894188506	32.57475484818395	23.244035379012004	19.36681487861554
8	24.501608832710993	35.53351709301839	22.112494808159582	17.852379266111036
9	24.16718797676913	15.054143114165381	19.80083742982724	40.977831479238255
10	14.725539160508744	28.739885478460625	33.64016642463591	22.894408936394726
11	31.950305285319057	22.868181850652565	25.529363620684904	19.652149243343473
12	20.673576874445736	26.023290060342706	29.552108729129312	23.751024336082242
13	28.830098489340273	20.84145063337902	26.783977597782638	23.544473279498074
14	21.431917240788117	21.908903228176666	26.890620806345115	29.7685587246901
15	23.778271930997253	28.625180247432652	22.896552005657703	24.69999581591239
16	24.443133657106877	27.099008779440414	23.90981556337719	24.54804200007552
17	21.151685421924416	29.940004265728344	25.45190697446584	23.4564033378814
18	23.900426878987002	25.82082104044992	26.410675342355315	23.868076738207762
19	23.604581269778745	27.164423417419886	26.85857681831773	22.372418494483636
20	22.83440299703134	26.893784384780943	25.888786951361514	24.383025666826207
21	24.840111725344244	25.661825711320407	26.04084281811567	23.45721974521968
22	21.643570843236525	29.62752435700268	26.21596219217616	22.51294260758463
23	23.97114816466528	25.485379675335214	26.45343467669759	24.09003748330192
24	23.049730432501992	27.320867473617287	26.675191319957182	22.954210773923542
25	22.509881080066087	27.989096879997305	26.898580777893322	22.602441262043286
26	24.353635002648222	25.89572641373687	26.981344071811193	22.76929451180372
27	21.566216247934744	29.577111203864053	26.532320035758644	22.32435251244256
28	23.22954414875758	25.692849190174943	27.279230699365144	23.79837596170233
29	21.813791773267354	26.026045435109395	27.979708195607117	24.180454596016137
30	22.925228313414696	27.598037764962452	27.533031330652115	21.94370259097074
31	24.436908551152513	25.193003797314635	25.681215385604496	24.688872265928364
32	21.90808682083839	25.97308100903865	28.28310557269444	23.835726597428522
33	23.161170034176852	25.626618144857193	27.899700276455935	23.312511544510016
34	21.737355636221135	26.964097466790083	26.571711689830522	24.72683520715826
35	21.584585413045986	25.859294236266244	31.05654335173992	21.499576998947855
36	22.904307875371337	25.855008097740285	25.68009282551436	25.56059120137401
37	22.49988009017219	26.900519745321734	27.777137124797047	22.82246303970903
38	22.19515605116017	25.543956901856614	28.23718265991634	24.023704387066882
39	23.539268682716553	25.755916657056872	26.93705397370964	23.76776068651693
40	21.698474236735677	25.079217024542228	30.965207780770136	22.257100957951963
41	22.61152379368162	26.02920901354522	26.510889343128863	24.8483778496443
42	22.888796135944066	25.312913625124118	29.020831653745322	22.777458585186494
43	23.128921944314897	24.82541639325525	29.084103222461817	22.961558439968037
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	9.0
1	10.5
2	12.0
3	81.0
4	150.0
5	150.0
6	185.5
7	221.0
8	249.5
9	278.0
10	402.5
11	527.0
12	527.0
13	841.0
14	1155.0
15	2743.0
16	4331.0
17	5008.5
18	5686.0
19	5686.0
20	5754.0
21	5822.0
22	7070.5
23	8319.0
24	10942.5
25	13566.0
26	13566.0
27	17201.0
28	20836.0
29	25013.5
30	29191.0
31	33444.0
32	37697.0
33	37697.0
34	43785.0
35	49873.0
36	53921.5
37	57970.0
38	61863.5
39	65757.0
40	65757.0
41	69131.5
42	72506.0
43	73762.5
44	75019.0
45	76047.0
46	77075.0
47	77075.0
48	76748.5
49	76422.0
50	74493.0
51	72564.0
52	83008.0
53	93452.0
54	93452.0
55	75057.0
56	56662.0
57	56738.5
58	56815.0
59	47525.0
60	38235.0
61	38235.0
62	30369.5
63	22504.0
64	18456.0
65	14408.0
66	11945.0
67	9482.0
68	9482.0
69	7645.5
70	5809.0
71	4698.5
72	3588.0
73	2698.5
74	1809.0
75	1809.0
76	1428.5
77	1048.0
78	801.0
79	554.0
80	421.5
81	289.0
82	289.0
83	216.5
84	144.0
85	115.5
86	87.0
87	52.5
88	18.0
89	18.0
90	14.5
91	11.0
92	6.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	979903.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.44462820570353
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.8594431754368	36.749825205257295
2	13.693962402106772	13.267978344658907
3	4.402599217164379	6.3984684664274925
4	1.8565151832497544	3.597527512431116
5	0.9644977715467743	2.336236797390653
6	0.583219795702895	1.6952319699019867
7	0.404346920463364	1.371190535957819
8	0.2906936523276353	1.1266036727016269
9	0.21606712069018202	0.9420562196381466
>10	1.4865487531002297	14.287911295814423
>50	0.16435689779252136	5.442112547925909
>100	0.07290295994092182	5.788406458073181
>500	0.0021070219468649672	0.6949190760511875
>1k	0.0018963197521784706	1.7852724207014206
>5k	4.2140438937299344E-4	1.5038546926354504
>10k+	4.2140438937299344E-4	3.0124047844334085
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGT	17004	1.735273797508529	No Hit
ATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATG	12508	1.2764528733966525	No Hit
TCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTT	7879	0.8040591772859149	TruSeq Adapter, Index 7 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCC	6854	0.6994569870691283	No Hit
CATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTC	3310	0.33778853621225774	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2811	0.2868651284872074	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2556	0.2608421445796166	No Hit
CTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCGTCTTC	2441	0.24910628909187948	RNA PCR Primer, Index 7 (95% over 22bp)
CACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTATGCCG	1701	0.1735886103012237	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1496	0.15266817225786633	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTA	1152	0.11756265671193984	No Hit
TATACACATCTCCGAGCCCACGAGACTAGCGCTCATCTCGTAT	1021	0.10419398654764808	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1002	0.10225501911923936	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	2.0410183456934003E-4	0.0
11	0.0	0.0	0.0	2.0410183456934003E-4	0.0
12	0.0	0.0	0.0	2.0410183456934003E-4	0.0
13	0.0	0.0	0.0	2.0410183456934003E-4	0.0
14	0.0	0.0	0.0	2.0410183456934003E-4	0.0
15	0.0	0.0	0.0	3.0615275185401006E-4	0.0
16	0.0	0.0	0.0	3.0615275185401006E-4	0.0
17	0.0	0.0	0.0	3.0615275185401006E-4	0.0
18	0.0	0.0	0.0	5.102545864233501E-4	0.0
19	0.0	0.0	0.0	5.102545864233501E-4	0.0
20	0.0	0.0	0.0	5.102545864233501E-4	0.0
21	0.0	0.0	0.0	7.143564209926901E-4	0.0
22	0.0	0.0	0.0	9.184582555620301E-4	0.0
23	0.0	0.0	0.0	0.0012246110074160402	0.0
24	0.0	0.0	0.0	0.0017348655938393901	0.0
25	0.0	0.0	0.0	0.0017348655938393901	0.0
26	0.0	0.0	0.0	0.0017348655938393901	0.0
27	0.0	0.0	0.0	0.0022451201802627403	0.0
28	0.0	0.0	0.0	0.008980480721050961	0.0
29	0.0	0.0	0.0	0.021940947216204053	0.0
30	0.0	0.0	0.0	0.042044977921284046	0.0
31	0.0	0.0	0.0	0.09745862600685987	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	2275	0.0	32.77143	1
TTATACA	2410	0.0	31.626556	2
TATACAC	2445	0.0	31.173824	3
CCGTCTT	1120	0.0	25.602678	37
GGTATCA	590	0.0	25.398306	1
GCCGTCT	1135	0.0	25.264317	36
CGTCTTC	385	0.0	24.987013	37
GATTACG	105	1.8189894E-12	24.666666	1
GTATCAA	1515	0.0	22.712872	1
TATCCCG	70	5.1006573E-6	21.142857	10
CCACGTT	115	1.3460522E-10	20.913044	37
TTACGGG	115	3.0486262E-9	19.304348	3
ATACACA	4115	0.0	18.927097	4
TACACAT	4130	0.0	18.544794	5
AGGACCG	60	9.2354877E-4	18.5	5
TATACAG	180	0.0	18.5	5
CGATCAT	50	0.007034159	18.5	29
TGCCGTC	1585	0.0	18.32492	35
CCCGTAT	75	2.0668747E-4	17.266666	13
TATCGTG	65	0.0015797097	17.076921	37
>>END_MODULE