FastQCFastQC Report
Fri 10 Feb 2017
ERR1631184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347536
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGT239056.878424105704157No Hit
ATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATG172604.966391970903734No Hit
TCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTT155504.474356613415589RNA PCR Primer, Index 39 (95% over 23bp)
ACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCC87842.5275079416233135No Hit
CTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTC66641.9174991943280695RNA PCR Primer, Index 39 (95% over 24bp)
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT55681.602136181575434No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT51031.4683370931356752No Hit
CATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTC47511.3670526218866534RNA PCR Primer, Index 39 (95% over 21bp)
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA30260.8707011647714193No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20540.5910179089360527No Hit
CACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCG18920.5444040329634916No Hit
TTATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA18900.543828553013213No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA17660.5081487960959441No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15070.4336241425348741No Hit
TATACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTAT14710.42326550342986047No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT14480.41664748400165735No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA13420.3861470466368952No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT12650.3639910685511717No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC12240.35219372957046174No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11980.3447124902168408No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10730.3087449933244326No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10190.29320703466691217No Hit
ACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGT10090.29032963491551955No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8960.2578150177247825No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8900.2560885778739469No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT7990.22990424013627367No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG7640.21983334100639934No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG7520.21638046130472816No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7510.21609272132958887No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG7270.20918696192624647No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7270.20918696192624647No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC7250.20861148197596793No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7150.2057340822245753No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7140.205446342249436No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG7120.20487086229915746No Hit
TCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCTTCT6830.19652640302011878Illumina PCR Primer Index 11 (95% over 24bp)
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6790.19537544311956173No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6520.18760646379080154No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6040.17379494498411674No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5810.16717692555591362No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5800.16688918558077437No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA5720.16458726577966024No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5560.15998342617743197No Hit
ATCTCCGAGCCCACGAGACCTCTCTACATCTCGTATGCCGTCT5440.15653054647576078Illumina PCR Primer Index 11 (95% over 21bp)
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5140.1478983472215828No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5110.147035127296165No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4990.1435822475944938No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4780.1375397081165692No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC4770.13725196814142995No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.1366764881911514No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4730.1361010082408729No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4510.12977072878780901No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4440.12775654896183417No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG4400.1266055890612771No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG4390.12631784908613786No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT4330.12459140923530225No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4150.11941208968279544No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4150.11941208968279544No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4110.11826112978223838No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG4090.11768564983195985No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA4030.11595920998112424No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA3870.11135537037889601No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA3840.11049215045347821No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3820.10991667050319967No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG3810.1096289305280604No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3770.10847797062750333No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3730.10732701072694627No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3700.10646379080152847No Hit
GTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGG3680.10588831085124994No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3620.10416187100041434No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3580.10301091109985727No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3570.102723171124718No Hit
ATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCTC3560.10243543114957873No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG3540.10185995119930023No Hit
CTTTACACATCTCCGAGCCCACGAGACCTCTCTACATCTCGTA3510.10099673127388242No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA3490.1004212513236039No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTTGTG251.2318535E-437.01
GGTGTTA200.001840170137.012
CTGTACT200.001840170137.04
CCTGTAC200.001840170137.03
GTACTTG200.001840170137.06
TTGGTGT200.001840170137.010
TGGTGTT200.001840170137.011
CGCCGAG403.8202415E-837.02
TTACGGG850.036.9999963
GATTACG850.036.9999961
CTTATAC27100.035.566421
TTATACA29000.033.108622
TCGCCGA451.07627784E-732.8888851
CTTCTAC451.07627784E-732.8888851
CGTCTTC8900.032.01123437
ACCATAA352.38355E-531.7142872
TATACAC30550.031.489363
ATTACGG1000.031.452
TACTTGG250.00549113229.67
ACTTGGT250.00549113229.68
Produced by FastQC (version 0.11.5)