FastQCFastQC Report
Fri 10 Feb 2017
ERR1631183.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631183.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328765
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGT152364.6343132632731585No Hit
ATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATG124953.8005870454580015No Hit
TCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTT81412.476236825696166RNA PCR Primer, Index 47 (95% over 22bp)
ACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCC68162.073213389503141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59431.8076741745623774No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT57051.7352820403631772No Hit
CATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTC32450.987027207884051No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA29120.8857390537313887No Hit
CTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGTCTTC28490.866576429972777RNA PCR Primer, Index 47 (95% over 23bp)
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT20230.6153331406932003No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA19160.5827870971666692No Hit
CACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCG15590.4741988958678691No Hit
TATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCGAGCT15320.4659863428284641No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA15110.4595988015755935No Hit
CTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCC11980.3643940200447128No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT11910.3622648396270892No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG11830.35983149057837666No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT11580.3522272748011498No Hit
TTATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTA11550.3513147689078825No Hit
TCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCC10430.31724788222590605No Hit
GTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAG10330.31420619591501525No Hit
TATACACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTAT9690.29473940352531447No Hit
GAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTGACTG8570.260672516843338No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8220.25002661475522026No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT8200.24941827749304213No Hit
GTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATGG8170.2485057715997749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7640.2323848341520539No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7640.2323848341520539No Hit
ACATCTCCGAGCCCACGAGACATGCGCAGATCTCGTATGCCGT7340.22325977521938162No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG7240.22021808890849087No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG7110.21626389670433288No Hit
CGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTG6990.21261387313126398No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6970.21200553586908583No Hit
GGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGAGGATG6790.20653050050948246No Hit
GTCTGAATATGGGGGGACCATCCTCCAAGGCTAAATACTCCTG6660.20257630830532447No Hit
GAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCG6600.20075129651879No Hit
GTTAATACCTTTGCTCATTGACGTTACCCGCAGAAGAAGCACC6590.20044712788770094No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG6310.19193040621720683No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA6140.18675953948869253No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6080.18493452770215807No Hit
GGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAG6050.18402202180889085No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5880.17885115508037658No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA5780.1758094687694858No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.16090520584612109No Hit
AAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGGGACACGT5290.16090520584612109No Hit
CGTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCT5280.160601037215032No Hit
GTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTGAC5140.15634267637978497No Hit
CCGTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCT5030.1529968214378051No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4910.1493467978647362No Hit
ATATTGCACAATGGGCGCAAGCCTGATGCAGCCATGCCGCGTG4790.1456967742916673No Hit
GTATGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4710.1432634252429547No Hit
GTATTAGCTACCGTTTCCAGTAGTTATCCCCCTCCATCAGGCA4690.14265508798077656No Hit
GAGTAGGGCGGGACACGTGGTATCCTGTCTGAATATGGGGGGA4650.14143841345642022No Hit
GTCCAGACTCCTACGGGAGGCAGCAGTGGGGAATATTGCACAA4460.1356592094657278No Hit
GTAATACGGAGGGTGCAAGCGTTAATCGGAATTACTGGGCGTA4430.13474670357246057No Hit
GTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAGGGTG4370.1329216917859261No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4280.13018417410612443No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC4270.12988000547503536No Hit
ATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATAC4260.1295758368439463No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG4240.12896749958176815No Hit
GATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCGCCGCTACTGG4130.12562164463978828No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC4120.12531747600869922No Hit
TTTGTGTACGGGGCTGTCACCCTGTATCGCGCGCCTTTCCAGA4040.12288412695998661No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA4030.12257995832889754No Hit
CTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGG3960.12045077791127401No Hit
GGTATTAACTTTACTCCCTTCCTCCCCGCTGAAAGTACTTTAC3950.12014660928018493No Hit
GGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGAGCTCG3910.11892993475582864No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3890.11832159749365048No Hit
ATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGAGCGTCA3890.11832159749365048No Hit
GTACACAAAAATGCACATGCTGTGAGCTCGATGAGTAGGGCGG3870.11771326023147233No Hit
TAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGGAA3850.11710492296929417No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG3710.11284656213404712No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA3560.10828403266771097No Hit
GTATTACCGCGGCTGCTGGCACGGAGTTAGCCGGTGCTTCTTC3540.10767569540553283No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT3520.10706735814335468No Hit
CCCTCACGGTACTGGTTCACTATCGGTCAGTCAGGAGTATTTA3460.10524234635682023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTC3430.104329840463553No Hit
AGTCAGGAGTATTTAGCCTTGGAGGATGGTCCCCCCATATTCA3420.10402567183246392No Hit
GCCCTACTCATCGAGCTCACAGCATGTGCATTTTTGTGTACGG3350.10189649141484039No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3300.100375648259395No Hit
GTCCCCCCATATTCAGACAGGATACCACGTGTCCCGCCCTACT3300.100375648259395No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGTCC200.001840025437.027
GTCCTAC200.001840025437.030
ATTACTT200.001840025437.02
CTATACA355.615002E-737.01
ATCCAAT200.001840025437.016
TTACGGG403.81915E-837.03
GATTACG355.615002E-737.01
CAGACAC251.231709E-437.018
CTTATAC15900.034.7893071
ACTCAGA507.2977855E-933.3000031
CTCAGAG507.2977855E-933.3000032
CGTCTTC3400.033.19117737
ATTACGG401.5954702E-632.3752
TTATACA17300.032.187862
GATACAC352.3831612E-531.7142873
ATGTAGG352.3831612E-531.7142874
TAGCAAC352.3831612E-531.7142871
GACAGAC303.593939E-430.83333416
CGACGAG303.593939E-430.83333424
TATACAC18500.030.53
Produced by FastQC (version 0.11.5)