FastQCFastQC Report
Fri 10 Feb 2017
ERR1631182.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1631182.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences884751
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT150801.7044343549767111No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG112161.267701308051644No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT98191.1098037752994911TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC55720.6297817125948431No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC35150.397286920274744RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC31650.35772776747356033No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT18180.20548154226443371No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT17470.19745668555333648No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG12950.14636886536437935No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA12600.142412950084261No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT9480.10714879101577732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC18600.031.8279571
CGTCTTC4800.031.60416637
CACGCCG250.005495667529.622
TTATACA20050.029.5261862
TATACAC20500.029.329273
GGTATCA4550.027.2417561
GATTACG754.620233E-1027.1333331
CCGTCTT15150.025.2772337
GCCGTCT15300.024.90849736
GTATCAA10600.020.0707531
TGCCGTC19150.019.90078435
CTACGTA759.261816E-619.7333349
ATGCCGT19850.019.10579334
GTCTAAT609.234527E-418.51
GCGTGCA500.007033632618.57
TATGCCG20700.018.4106333
TACACAT33550.018.3621465
ATACACA33000.018.1636374
AACCACG1502.5102054E-1017.26666635
GTGCTAG1301.3917088E-817.07692327
Produced by FastQC (version 0.11.5)