##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1631182.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	884751
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514048585421207	31.0	31.0	34.0	28.0	34.0
2	31.5129443199273	31.0	31.0	34.0	30.0	34.0
3	30.990054828985784	31.0	31.0	34.0	27.0	34.0
4	35.12976193301844	37.0	35.0	37.0	32.0	37.0
5	35.314366414957426	37.0	35.0	37.0	33.0	37.0
6	35.5045114388116	37.0	35.0	37.0	33.0	37.0
7	35.506491656974674	37.0	35.0	37.0	33.0	37.0
8	35.534448675390024	37.0	35.0	37.0	33.0	37.0
9	37.311271758946866	39.0	37.0	39.0	34.0	39.0
10	37.14154603950716	39.0	37.0	39.0	33.0	39.0
11	37.20488476418789	39.0	37.0	39.0	33.0	39.0
12	37.14543526935827	39.0	37.0	39.0	33.0	39.0
13	37.21623937130334	39.0	37.0	39.0	34.0	39.0
14	38.37345987741184	40.0	38.0	41.0	34.0	41.0
15	38.377316329679196	40.0	38.0	41.0	34.0	41.0
16	38.375868464686675	40.0	38.0	41.0	34.0	41.0
17	38.32276651848938	40.0	38.0	41.0	34.0	41.0
18	38.374722379516946	40.0	38.0	41.0	34.0	41.0
19	38.3960990154292	40.0	38.0	41.0	34.0	41.0
20	38.38027987535476	40.0	38.0	41.0	34.0	41.0
21	38.35922536397246	40.0	38.0	41.0	34.0	41.0
22	38.28825398332412	40.0	38.0	41.0	34.0	41.0
23	38.288708348450584	40.0	38.0	41.0	34.0	41.0
24	38.24360413268818	40.0	38.0	41.0	34.0	41.0
25	38.210931663258926	40.0	38.0	41.0	34.0	41.0
26	38.11285322084971	40.0	37.0	41.0	33.0	41.0
27	38.025417320805516	40.0	37.0	41.0	33.0	41.0
28	38.032488236803346	40.0	37.0	41.0	33.0	41.0
29	37.964742622500566	40.0	37.0	41.0	33.0	41.0
30	37.89089698683584	40.0	37.0	41.0	33.0	41.0
31	37.866621230154024	40.0	37.0	41.0	33.0	41.0
32	37.783454327827826	40.0	37.0	41.0	33.0	41.0
33	37.75936845507945	40.0	37.0	41.0	33.0	41.0
34	37.691652227575894	40.0	37.0	41.0	33.0	41.0
35	37.688354124493785	40.0	37.0	41.0	33.0	41.0
36	37.67823602346875	40.0	37.0	41.0	33.0	41.0
37	37.61410272494747	40.0	37.0	41.0	32.0	41.0
38	37.59147149876067	40.0	37.0	41.0	32.0	41.0
39	37.532800754110475	40.0	37.0	41.0	32.0	41.0
40	37.463755339072804	40.0	37.0	41.0	32.0	41.0
41	37.421278981317904	39.0	37.0	41.0	32.0	41.0
42	37.35945141627418	39.0	37.0	41.0	32.0	41.0
43	36.72924020430607	39.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	2.0
9	8.0
10	14.0
11	21.0
12	9.0
13	14.0
14	12.0
15	14.0
16	17.0
17	13.0
18	21.0
19	28.0
20	66.0
21	151.0
22	276.0
23	527.0
24	1005.0
25	1704.0
26	2859.0
27	4486.0
28	6762.0
29	9619.0
30	13823.0
31	18401.0
32	24538.0
33	32244.0
34	42779.0
35	57861.0
36	81355.0
37	121074.0
38	214709.0
39	250337.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.844207014176874	22.2485196399891	14.891873532779279	25.015399813054746
2	13.992185371929503	21.721252646224755	40.69969968951717	23.586862292328576
3	14.330246589153333	28.41974747697375	33.80465238242172	23.4453535514512
4	11.680122429926612	19.458582132147914	38.038498967506115	30.822796470419362
5	12.439432111407617	38.03725567984665	36.318240951408924	13.205071257336808
6	27.916442027191827	40.04403498837526	17.881641275341874	14.157881709091033
7	24.834218893225326	32.85681508130536	23.497797685450482	18.81116834001883
8	24.465527589118295	35.7685947797742	21.89802554617062	17.867852084936892
9	23.81393182940737	15.24530630652014	19.971155726300395	40.9696061377721
10	14.400322802686857	28.88123325093727	34.029913501086746	22.688530445289125
11	31.377867897295395	23.118594949313422	25.591607130141703	19.91193002324948
12	20.35205385470036	26.301637409847515	29.58787274611727	23.758435989334853
13	28.2881850373721	20.751488271841456	26.875471177766403	24.08485551302005
14	21.60924373072198	21.960981112199928	26.371826649531904	30.05794850754619
15	23.644844707720026	29.123787370683957	22.5534641950108	24.67790372658522
16	24.879655405871258	27.285304000786663	23.98403618645246	23.851004406889622
17	21.079264109336975	30.51446113087185	25.31424095592997	23.0920338038612
18	23.43416396251601	26.19539282803862	26.355550883807986	24.014892325637383
19	22.99290986955652	27.821613086619852	26.83975491409447	22.345722129729158
20	22.51921727130006	27.470779914348782	25.664056892843295	24.34594592150786
21	24.879203301267815	25.93158979193016	25.94605713924031	23.24314976756172
22	22.382116550306243	29.563233045229676	26.051736590294894	22.002913814169183
23	23.036198885336102	25.988781024265585	26.240603288382836	24.73441680201548
24	23.16730922033431	28.882815617049317	26.33475407204965	21.615121090566724
25	22.50011585180463	27.89813179075243	27.159053790275458	22.442698567167486
26	25.189573111530816	26.66422530180808	27.05179197310882	21.094409613552287
27	21.94628771258806	30.01918053780103	26.222066999641708	21.8124647499692
28	22.86451216217896	25.81562496114726	27.40680711296173	23.913055763712048
29	23.24733173514356	26.557980719999186	28.11333358199086	22.081353962866388
30	21.76533284506036	27.44704442266807	27.716385740168704	23.07123699210286
31	24.994263922843828	26.65778281120903	25.749391636742992	22.59856162920415
32	23.915203260578398	26.268746799947102	28.024947132017935	21.79110280745656
33	22.55956760715727	26.131420026651565	27.86490210239943	23.444110263791735
34	21.86163112559353	29.26360071929842	26.538992326654622	22.33577582845343
35	21.96143321680337	25.74238401538964	31.132714176078917	21.16346859172807
36	25.00986153166258	25.990024311925048	25.380022175730797	23.620091980681572
37	22.131537573848462	26.8539962091029	27.89428890162317	23.12017731542547
38	22.28005393607919	25.771657788462516	27.945263695661264	24.003024579797028
39	23.517916340303657	25.96866236941241	26.89954574789969	23.613875542384243
40	21.531255686628214	25.278411666107186	30.917625410991338	22.272707236273256
41	22.326281631781146	26.190758755853345	26.446310882948985	25.036648729416527
42	22.75193811592188	25.61941156325339	29.177361766191844	22.451288554632885
43	23.037555199146425	24.9757276341027	29.229918926341984	22.756798240408884
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	12.5
2	18.0
3	71.0
4	124.0
5	124.0
6	177.0
7	230.0
8	219.5
9	209.0
10	321.5
11	434.0
12	434.0
13	666.5
14	899.0
15	1989.0
16	3079.0
17	3664.0
18	4249.0
19	4249.0
20	4638.0
21	5027.0
22	6092.0
23	7157.0
24	9571.5
25	11986.0
26	11986.0
27	15589.0
28	19192.0
29	23127.0
30	27062.0
31	31312.5
32	35563.0
33	35563.0
34	40672.0
35	45781.0
36	49777.0
37	53773.0
38	58123.5
39	62474.0
40	62474.0
41	65997.5
42	69521.0
43	70677.5
44	71834.0
45	71312.5
46	70791.0
47	70791.0
48	69551.5
49	68312.0
50	78273.5
51	88235.0
52	70981.0
53	53727.0
54	53727.0
55	58209.0
56	62691.0
57	52237.0
58	41783.0
59	34264.5
60	26746.0
61	26746.0
62	23350.5
63	19955.0
64	16626.0
65	13297.0
66	10836.0
67	8375.0
68	8375.0
69	6708.5
70	5042.0
71	4124.0
72	3206.0
73	2524.5
74	1843.0
75	1843.0
76	1408.5
77	974.0
78	778.5
79	583.0
80	443.0
81	303.0
82	303.0
83	227.0
84	151.0
85	113.0
86	75.0
87	52.5
88	30.0
89	30.0
90	19.5
91	9.0
92	6.0
93	3.0
94	2.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	884751.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.477952325073176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.83686037461652	41.09838092148031
2	11.552963903496112	11.894458500749282
3	3.415381307537499	5.274505083640843
4	1.4369188090839395	2.958785517960958
5	0.8013776817605147	2.0626641048022716
6	0.49543659540202095	1.5302436862921078
7	0.35851821304821824	1.291904843527662
8	0.2636955035186026	1.085960364677343
9	0.20163029755910322	0.9341563360534048
>10	1.417708017647061	14.460473662897572
>50	0.14978637162013725	5.254914680038899
>100	0.06642373269282231	5.585328393516438
>500	0.0010997306724876968	0.39900055229137577
>1k	0.0013196768069852361	1.4492642080958031
>5k	4.3989226899507876E-4	1.742626986468946
>10k+	4.3989226899507876E-4	2.9773321575068157
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGT	15080	1.7044343549767111	No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATG	11216	1.267701308051644	No Hit
TCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT	9819	1.1098037752994911	TruSeq Adapter, Index 1 (95% over 21bp)
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCC	5572	0.6297817125948431	No Hit
CTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTC	3515	0.397286920274744	RNA PCR Primer, Index 1 (95% over 22bp)
CATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTC	3165	0.35772776747356033	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1818	0.20548154226443371	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1747	0.19745668555333648	No Hit
CACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCG	1295	0.14636886536437935	No Hit
TTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTA	1260	0.142412950084261	No Hit
TATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTAT	948	0.10714879101577732	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.130261508605246E-4	0.0
3	0.0	0.0	0.0	1.130261508605246E-4	0.0
4	0.0	0.0	0.0	1.130261508605246E-4	0.0
5	0.0	0.0	0.0	1.130261508605246E-4	0.0
6	0.0	0.0	0.0	1.130261508605246E-4	0.0
7	0.0	0.0	0.0	1.130261508605246E-4	0.0
8	0.0	0.0	0.0	1.130261508605246E-4	0.0
9	0.0	0.0	0.0	1.130261508605246E-4	0.0
10	0.0	0.0	0.0	2.260523017210492E-4	0.0
11	0.0	0.0	0.0	3.390784525815738E-4	0.0
12	0.0	0.0	0.0	3.390784525815738E-4	0.0
13	0.0	0.0	0.0	4.521046034420984E-4	0.0
14	0.0	0.0	0.0	4.521046034420984E-4	0.0
15	0.0	0.0	0.0	6.781569051631476E-4	0.0
16	0.0	0.0	0.0	7.911830560236722E-4	0.0
17	0.0	0.0	0.0	7.911830560236722E-4	0.0
18	0.0	0.0	0.0	7.911830560236722E-4	0.0
19	0.0	0.0	0.0	7.911830560236722E-4	0.0
20	0.0	0.0	0.0	9.042092068841968E-4	0.0
21	0.0	0.0	0.0	0.0012432876594657707	0.0
22	0.0	0.0	0.0	0.001695392262907869	0.0
23	0.0	0.0	0.0	0.002260523017210492	0.0
24	0.0	0.0	0.0	0.0024865753189315413	0.0
25	0.0	0.0	0.0	0.002599601469792066	0.0
26	0.0	0.0	0.0	0.0027126276206525905	0.0
27	0.0	0.0	0.0	0.003729862978397312	0.0
28	0.0	0.0	0.0	0.00836393516367882	0.0
29	0.0	0.0	0.0	0.01717997493079974	0.0
30	0.0	0.0	0.0	0.03232547914611004	0.0
31	0.0	0.0	0.0	0.07697080873601725	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	1860	0.0	31.827957	1
CGTCTTC	480	0.0	31.604166	37
CACGCCG	25	0.0054956675	29.6	22
TTATACA	2005	0.0	29.526186	2
TATACAC	2050	0.0	29.32927	3
GGTATCA	455	0.0	27.241756	1
GATTACG	75	4.620233E-10	27.133333	1
CCGTCTT	1515	0.0	25.27723	37
GCCGTCT	1530	0.0	24.908497	36
GTATCAA	1060	0.0	20.070753	1
TGCCGTC	1915	0.0	19.900784	35
CTACGTA	75	9.261816E-6	19.733334	9
ATGCCGT	1985	0.0	19.105793	34
GTCTAAT	60	9.234527E-4	18.5	1
GCGTGCA	50	0.0070336326	18.5	7
TATGCCG	2070	0.0	18.41063	33
TACACAT	3355	0.0	18.362146	5
ATACACA	3300	0.0	18.163637	4
AACCACG	150	2.5102054E-10	17.266666	35
GTGCTAG	130	1.3917088E-8	17.076923	27
>>END_MODULE